查询词典 sequencing problem

Most of characterized conotoxins are mainly from fish-hunting conus as well as several molluscivorous species, because they were believed to be toxic to vertebrateIn this study, we investigated the venom of Conus marmoreus and Conus vexllium from South China Sea by separating, purifying and protein sequencing technologies to character unknown conotoxins.

本文通过天然毒素的提取，分离纯化，蛋白质测序，化学合成与二硫键定位等手段对中国南海的食螺的大理石芋螺和食虫的菖蒲芋螺进行了新型小肽毒素的发现和鉴定研究。

The method of combining PCR amplification and sequencing to analyze the CHD gene is an accurate and reliable way to determine sex of monomorphic avian.

结论］结合PCR扩增和测序分析CHD基因，是单态性鸟类性别鉴定的有效和准确的方法。

Employing HP-8-8 polyacrylic resins as a porous multimolecular carrier for fixed cultivation of sludge,experimental investigation of wastewater treating was carried out by means of fluidization with biofilm sequencing batch reactor.

采用HP88型聚丙烯酸酯类多孔高分子载体对污泥实行固定化培养，以流态化方式进行生物膜SBR 废水处理试验研究。

Methods By using immunohistochemical fechnique, MDT-PCR-SSCP and direct sequencing the mP53 expression and point muta- tion in exon 6 of p53 gene in both Solt-Farber model and DEN-induced liver cancer model were detected.

通过DEN诱发的启动模型和肝癌模型，应用免疫组化、MDT－PCR－SSCP和直接测序技术分别研究mP53表达和 p53基因第6外显子点突变。

The myrosinase gene was amplified from total RNA by RT-PCR and inserted into Escherichia coli expression vector pGEX-4T-1.The result of sequencing was EF583560 in GenBank.

实验通过RT-PCR程序从提取的油菜总RNA中扩增出硫代葡萄糖苷水解酶（又称黑芥子酶，EC3.2.1.147）基因，酶解后插入大肠杆菌表达载体pGEX-4T-1，获得克隆菌株。

Method］ With filter paper as the sole carbon source, a Myxobacteria CMC0606 was obtained; through observing its morphology, 16S rDNA cloning and sequencing, the bacteria was identified; adding XAD-16 into fermentation broth, crude extracts of fermentation products were obtained.

方法］以滤纸为唯一碳源，得到1株粘细菌CMC0606；通过观察其形态及16S rDNA克隆与测序进行菌种鉴定；向其发酵液中加入大孔吸附树脂XAD-16，收获发酵产物粗提物。

Methods PCR- SSCP and DNA sequencing were used to analyze the PTCH gene mutations in 12 OKCs, including 10 sporadic and 2 nevoid basal cell carcinoma syndrome associated OKC.

方法采用PCR—SSCP筛查与DNA直接测序的方法对12例OKC进行PTCH基因突变的检测，其中2例为痣样基底细胞癌综合征相关OKC，10例为散发OKC。

A submerged membrane sequencing batch bioreactor was used to treat coke wastewater.Long running performance showed that due to the membrane interception,the nitrobacter is enriched in reactor in the interest of improving the nitrification rate;the maximum ammonia nitrogen loading can be 0.19 kg/(m3·d) with effluent ammonia nitrogen＜1 mg/L (removal rate 99%).Long sludge retention time may result in the accumulation of metabolic products and high molecular materials,and thus inhibiting activity of nitrate bacteria and causing a ccumulation of NO2-,which is beneficial to the running of short-cut denitrif ication.However,too long retention time will affect the activity of nitrite bacteria,detrimental to the treatment effect of ammonia nitrogen.

采用浸没式膜生物反应器处理焦化废水的试验结果表明：膜的截留作用可使硝化菌在反应器内富集而有利于提高系统的硝化能力，其去除氨氮的最高负荷为0.19kg/(m3·d)，出水氨氮＜1mg/L(去除率为99%)；泥龄长可能使微生物的代谢产物或其他大分子物质积累，从而抑制硝酸盐细菌的活性，导致NO2-积累而有利于短程脱氮的进行，但泥龄过长也会影响亚硝酸盐细菌的活性，从而影响对氨氮的处理效果。

A submerged membrane sequencing batch bioreactor was used to treat coke wastewater.Long running performance showed that due to the membrane interception,the nitrobacter is enriched in reactor in the interest of improving the nitrification rate;the maximum ammonia nitrogen loading can be 0.19 kg/(m3·d) with effluent ammonia nitrogen＜1 mg/L (removal rate 99%).Long sludge retention time may result in the accumulation of metabolic products and high molecular materials,and thus inhibiting activity of nitrate bacteria and causing a ccumulation of NO2-,which is beneficial to the running of short-cut denitrif ication.However,too long retention time will affect the activity of nitrite bacteria,detrimental to the treatment effect of ammonia nitrogen.

简介： 采用浸没式膜生物反应器处理焦化废水的试验结果表明：膜的截留作用可使硝化菌在反应器内富集而有利于提高系统的硝化能力，其去除氨氮的最高负荷为0.19kg/(m3·d)，出水氨氮＜1mg/L(去除率为99%)；泥龄长可能使微生物的代谢产物或其他大分子物质积累，从而抑制硝酸盐细菌的活性，导致NO2-积累而有利于短程脱氮的进行，但泥龄过长也会影响亚硝酸盐细菌的活性，从而影响对氨氮的处理效果。

In this paper, a research was made on the treatment of nitrophenol removal ammonia wastewater from a coke-plant by sequencing batch reactors.

试验表明SBR法处理焦化废水可以取得良好的脱氮效果。在低溶解氧下，氨氮及总氮的去除效率均较高。对氮平衡的分析证实了好氧条件下也可发生反硝化作用，初步探讨了好氧反硝化的机理。

We were supposed to participate in a siege of this unit.

当然我们也参加了围困这支部队的任务。

Sometimes we eat pomelos.

有时我们也会吃柚子。