查询词典 sequencing problem

Total RNA was extracted from venom cells from Gloydius intermedius, FLE gene was amplified by RT-PCR. Then it was connected to pMD18-T vector by TA cloning. At last, sequencing and analysis the genesequence.

从中介蝮蛇毒腺细胞中提取总RNA，通过RT-PCR法扩增纤溶酶基因，与pMD18-T载体连接进行TA克隆，再进行序列的测定并且对该序列进行分析。

Methods:Total RNA was extracted from venom cells from Gloydius intermedius,FLE gene was amplified by RT-PCR.Then it was connected to pMD18-T vector by TA cloning.At last,sequencing and analysis the gene sequence.

材料及方法：从中介蝮蛇毒腺细胞中提取总RNA，通过RT-PCR法扩增纤溶酶基因，与pMD18-T载体连接进行TA克隆，再进行序列的测定并且对该序列进行分析。

The invention also relates to a process for manufacturing the interpolymer product wherein the process employs two or more single site or metallocene catalyst systems in at least one reaction environment and wherein at least two of the catalyst systems have different monomer incorporation capabilities or reactivities and the same or, optionally, different monomer sequencing and/or tacticity characteristics.

本发明也涉及一种制备该共聚体产物的方法，其中该方法在至少一种反应环境中使用二种或多种的单一位置或二茂金属催化剂体系，且其中至少二种该催化剂体系具有不同单体结合的能力或反应性，和相同或可选不同的单体序列和/或立体规整度特性。

The results of cDNA sequencing examined that the sBD-1 cDNA contain a open reading frame of 192 bases which encoded a 64 amino acid prepro-peptide and the prepro-peptide contained the β–defensin consensus sequence of six invariantly spaced cysteine residues.

我们采用 cDNA 末端快速扩增即 RACE 技术，主要包括锚式 PCR RACE 技术和反向嵌套 PCR RACE 技术分别扩增出蒙古绵羊 sBD-1 的 3ˊ末端和 5ˊ末端，经测序得到 sBD-1 的 cDNA 全长序列。

Through PCR and sequencing, a fragement sequence with lamin gene feature was obtained. It was of of transcriptional activity. In addition, we also cloned and identified giardial DNA topoisomerase gene.Our phylogenetic analysis showed that although Giardia diverged early but not first, thus, it is not as primitive as thought before.

然后进行了核纤层蛋白基因的扩增和鉴定，获得了一个具有明显核纤层蛋白基因特征的基因序列片段，并证明它是表达的且不具内含子；此外还克隆鉴定了作为核骨架重要成分的DNA拓扑异构酶II的基因；在此基础上结合已报导的不同进化地位生物上的数据进行了分子系统学分析，结果显示贾第虫虽然分支较早，但不象过去所认为的那么原始。

The HPV positivity amongst the group of women with no cervical abnormality was 43%, while that in women with cervical dysplasia and cervical cancer was 81.2% PCR products from 89 HPV positive cases were genotyped by LiPA, of which 49 cases were single HPV infected concerning 11 different HPV types and the commonest type was HPV-16, occurring in 25(51%) of all single HPV infected cases. The other 40 specimens contained multiple HPV types comprising 16 different HPV types. Although double infections(n=25) prevailed, triple(n=9),quadruple(n=3) and quintuple(n=3) infections were also found. PCR products of four HPV positive cases failed to be classified by LiPA. Sequencing analysis revealed that these 4 cases were HPV-66 which was not included in LiPA probes. Follow-up data of 12 cases confirmed the HPV genotyping results. Conclusion The LiPA for HPV genotyping is easy to perform.

结果 155例妇女子宫颈刮片中HPV DNA检出率为60%，其中在宫颈细胞学检查正常或仅有炎症病变的妇女中HPV DNA阳性率为43%，而在宫颈细胞增生不良或宫颈细胞癌的妇女其阳性率为81.2%。93例HPV阳性的标本中，89例利用LiPA成功地作出了基因分型，其中单独一型HPV感染为49例，涉及11种不同基因型HPV，最常见的型别为HPV 16，占单独感染的51%(25/49)；另40例为不同型别HPV重叠感染，涉及所有16种HPV基因型别，二重感染占主导地位(n=25)，同时也检测出其它多重感染。4例HPV阳性的标本应用LiPA未能作出基因分型，DNA序列分析显示这4例为HPV 66感染，此型别不包括在LiPA所标记的探针内。12例随诊资料显示了LiPA的物异性及高度的重复性。

Objective A prospective study was organized to determine the accuracy and costeffectiveness of the TB AccuProbe DNA hybridization test,the PCR bases LiPA assay,and a verifying automated DNA sequencing of the rpoβ gene,for the routine rapid and direct identification of the Mycobacterium tuberculosis complex and Non Tuberculosis Mycobacterium and rifampin susceptibility.

目的用 3种先进的技术-TBAccuProbeDNA杂交法、INNO LIPATMRIF TB探针试验和rpoβDNA基因序列测定的前瞻性研究，以鉴定结核分枝杆菌复合物和非结核分枝杆菌及其成本效益。

We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.

从杂草赛葵上采集了2组病毒样本Fz1和Fs1，用双生病毒通用引物PA、PB对杂草样本进行特异性的PCR检测，结果发现：赛葵样本Fz1和Fs1都出现约500bp的条带，说明这些杂草赛葵上存在双生病毒。

Both honey bees and bumble bees can be infected by microsporidia, and the researches about the rRNA of microsporidia are mainly concentrated on sequencing the rRNA, the secondary structures of rRNA and detection and diagnosis of microsporidia by rRNA gene.

蜜蜂和熊蜂都被微孢子虫感染，关于蜜蜂和熊蜂微孢子虫rRNA基因研究主要有rRNA基因序列的测定分析、二级结构的研究和用rRNA基因检测诊断微孢子虫等。

The optimized microvolume DNA sequencing can detect known and unknown mutation correctly and reliably at the same time.

4优化的微量DNA自动测序技术具有准确可靠的优点，可同时检测已知和未知基因突变。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。