查询词典 sequencing problem

The identification was made by means of restriction enzym eanalysis, PCR and DNA sequencing. Results: The murine entire coding cDNA of 2508bp was enamplified from spleen cells. There was 100% homogenous nucleotide sequence as compared with standard sequence (NM021297) of TLR4 from Genbank.

结果：扩增得到的小鼠TLR4基因cDNA全长2508bp，编码536个氨基酸，Blast结果分析表明，该cDNA与基因库中发表的序列(NM021297)具有100%的同源性。

In this research, we attempted to construct an SSH (suppression subtractive hybridization) library using the leaves of wild H. villosa (Gh21) inoculated by Erysiphe graminis and its susceptible EMS-induced mutant M14S with no inoculation.We screened 218 positive clones in the SSH library, and gained 60 ESTs after sequencing.

本研究应用抑制性消减杂交技术，以野生型簇毛麦(Gh21)及其感病突变体(M14S)为材料，建立白粉菌诱导的叶片的SSH文库，获得218个阳性克隆，对其中部分克隆测序，获得60个EST序列。

Subtilis secrects extracellularly. The sequencing data of the three cloned fragments showed that all fragments contain promoters, ribosome binding sites and signal peptide function fragments.

对外源插入片段进行序列测定发现，所有片段中均含有启动子区、核糖体结合位元点和信号肽编码区域。

With the development of genome sequencing and transformation, TGR has been applied widely to filamentous fungi, and many new systems and approaches have been established.

随着基因组测序和转化技术的进展，该技术在丝状真菌功能基因组学中的应用越来越广泛，新的体系与方法不断建立和完善。

Denaturing high performance liquid chromatography and direct sequencing in conjunction with DNA pooling were used as possible strategies for augmenting the efficiency, economy, and throughput of SNPs detection in the finless porpoise.

选择2个已知的江豚SNPs，将扩增产物按基因频率制备成0-50%的11 个DNA池，分别用于直接测序和DHPLC分析，以探讨两种方法检测SNPs时的效率及制备DNA池时对基因频率的要求。

Sequencing showed that the Nephila clavata flagelliform silk protein is composed of 10 of GPGG and 26 of GG amino acid motifs.

分析测序cDNA 序列发现，整个基因可分为A、B、C 三个部分，其中A 和C 部分为非重复序列，B 部分为重复序列区域。

On the basis of above-mentioned principle, taken Visual Basic 6.0 as the foreground explointment software and the Microsoft Access 2003 as data pool, this system has made the following three functions to come true: the function of formational management, inquest and matching, and it also has completed the normalization, systematization and sequencing of recruiting management.

基于上述原则，本系统采用visual basic6.0作为系统的前端开发软件，以Microsoft Access 2003作为数据源，实现了招聘中介管理系统的三大功能：信息管理功能、信息查询功能和信息匹配功能，实现了招聘管理工作的规范化、系统化和程序化，提高了信息处理的速度和准确性。

Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,and 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos.

测序和基因数据库比对结果表明，72个原肠期斑点杂交阳性克隆中，包括19个已知基因的cDNA片段和31个没有同源性的cDNA片段；98个尾芽期斑点杂交阳性克隆中，包括52个已知基因的cDNA片段和37个没有同源性的cDNA片段。

In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料，采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库，初始文库滴度为3×10^6，插入片段平均长度大于1kb；随机挑取2004个阳性克隆进行5'EST测序，获得高质量序列1758条，经拼接获得1365条单基因簇，其中跨叠群175个和单条序列1195个；经ORF寻找共获得有效ORF1283条；经COG分析EST序列编码的蛋白质被分为22类；经Blast分析后，获得一批花色、花器官发育、花期调控以及其它育种价值基因（包括cDNA全长或片段）。

It was Eric Lander,while working for Colins in the tortoise-paced Human Genome Project, who saw that his team was losing and made it his business to beat Venter's harelike private venture at its own game .With $34 milltortoise-paced Human Genome Project, who saw that his team was losing and made it his business to beat Venter's harelike private venture at its own game .With $34 million from the Gemome Project and a $38 million loan from the Massachusetts Institute of Technologh's Whitehead Institute,Lander ordered dozens of special-purpose computers and state-of-the art capillary machines and built automated gene-sequencing pipeline so insatiable that he was soon grabbibg long stretches of DNA from other labs to fees its monstrous appetite .It was his lab's work that brought the race to a photo finish, and it was his name that appeared first on the Nature article that published the results.

这是埃里克Lander，当工作为Colins 在草龟踱步的人的染色体项目，看见他的队是丢失的并且做它他的事务摔打Venter's harelike 私有事业在它自己的游戏With $34 milltortoise 踱步了人的染色体项目，看见他的队是丢失和做它他的事务摔打Venter's harelike 私有事业在它自己的游戏With $34 百万从Gemome 项目和一笔$38 百万贷款从Technologh's Whitehead 学院马萨诸塞学院， Lander 定购了许多专用计算机和状态这艺术血丝机器和修造了自动化基因程序化管道很贪心他是grabbibg 长期很快舒展DNA 从其它实验室对费它是带来种族给相片结束的他的lab's 工作，并且这是出现首先在自然文章出版结果的他的名字。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。