查询词典 sequencing problem

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

This is also explainable in virtue of the explanatory power that speech act theory has in describing conversational sequencing.

这也是能够通过言语行为理论所具有的解释力解释的。

Influence of different carbon substrates （acetate, glucose, peptone and fecula） on the formation and characteristics of aerobic granules in Sequencing Batch Reactor is studied.

研究了不同的碳源基质（乙酸钠、葡萄糖、蛋白胨、淀粉）对序批式活性污泥法反应器中形成的好氧颗粒污泥的外观形态和特性的影响。

Influence of different carbon substrates(acetate,glucose,peptone and fecula) on the formation and characteristics of aerobic granule s in Sequencing Batch Reactor is studied.

研究了不同的碳源基质(乙酸钠、葡萄糖、蛋白胨、淀粉)对序批式活性污泥法反应器中形成的好氧颗粒污泥的外观形态和特性的影响。

By sequencing hundreds or thousands of genes, researchers fingered dozens of mutations, both known and new.

通过数百或数千基因的测序，研究人员确认了包括已知的和新发现的几十个突变。

The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae， App)鞭毛蛋白编码基因的重组表达质粒，对其编码蛋白进行生物信息学分析，并将其在原核细胞中进行表达。

A 1.2 kb fragment of the gyrB gene of 17 Gordonia strains including type strains were determined by direct sequencing of PCR amplified fragments. Total 25 strains (8 were retrieved from public database) of genus Gordonia form a distinct phyletic line in the gyrB-based tree and are separated from other closely-related species of genera to suborder Corynebacterineae.

在Gordonia菌种间之差异研究中则发现12种Gordonia gyrB基因序列相似度介於79.3%-97.2%，16S rDNA的序列相似度为96.2%-99.7%，可知Gordonia放线菌的16S rDNA保留性极高，较难利用此基因序列进行菌种间之区分。gyrB基因之变异程度无论是在菌属，菌种甚至各分离株间均较16S rRNA基因大。

Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments. Ligation of these four fragments resulted in a cloned DNA MgE717 coding for the 29～268 amino acid region of gE. Sequencing result showed that there is a mutation at 255 amino acid, Asn instead of Asp.

分四段分别合成，基因拼接得到了改造后的基因片段MgE717，经测序，除了在gE基因+763位核苷酸处存在一碱基置换，导致第255位的氨基酸残基由Asp突变为Asn，其余的序列与PrV鄂A株gE基因的氨基酸序列相符。

Results A 357 bp DNA band was seen in the 2% agarose gel after the PCR product was electrophoresed. After the 357 bp DNA band was recovered and ligated into pMD-18T, human transforming growth factor-beta 3 encoding gene acquired through four-cycle PCR was confirmed by sequencing.

结果：4次PCR后，经2％的琼脂糖凝胶电泳可见一357 bp的条带，将电泳产物回收，连接入pMD-18T载体，经测序分析证实，所获得DNA片段为人转化生长因子β3的编码基因。

Finally we need an sequencing schema that both allow random insertion at arbitrary positions and rarely need to change saved data. Then this class come into idea inspired by fractional arithmetic of the positional number system.

因为采集信息不足，所以只要是人不同或者被看待的事情不同，总结出来的规律就不会完全一样，在地域和时间上再存在差距的话，这种不同就会更加剧烈，甚至能够导致完全格格不入的价值观念体系。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。