查询词典 sequencing problem

Hepatica. Sequencing analysis showed that the gene, like that of other eggshell protein of schistosoma, has no introns.

通过从血吸虫基因组DNA中克隆该基因并进行测序分析发现该基因同其他血吸虫卵壳蛋白基因一样没有内含子，这可能有利于卵壳前体蛋白的迅速和大量形成。

Methods: According to the conserved regions of three genes reported, three pairs of specific primers were designed and used to amplify them from genomic DNA of soft hexaploid Chinese spring＇ s young leaves, Results: Through gene cloning and sequencing, they were 447bp, 447bp and 495bp in size, encoding 148, 148 and 164 amino acid residues respectively.

根据已报导的小麦3种基因的保守序列，设计合成了3对特异性引物，对六倍体软质小麦中国春基因组DNA进行基因扩增、克隆和序列测定，得到了3个基因的全长。

The products amplified by primer 2 had polymorphism. The sequencing results indicated that there were two nucleotide mutations( C→A and C→T )at exon 1183bp and 336bp of hircine GDF9 gene, but the two mutations did not cause amino acid change. The products amplified by primer 3 had polymorphism.

所设计的6对引物扩增产物中共有3对发现了多态，引物1经SSCP分析虽然没有发现多态但经克隆测序却在外显子1编码区前-4bp处检测到A→C的突变。

The further analysis was given to Rhesus boxes specific sequencing and RHD gene homozygosity determined by PCR-restriction fragment length polymorphism analysis to Rhesus boxes.

然后对Rh盒子基因进行特异性测序分析，同时对Rh盒子基因的扩增产物采用聚合酶链反应-限制性片段长度多态性（restrict fragment length polymorphism，RFLP）方法进行RHD基因的纯合性测定。

Six SNPs, which were C→T at the sites of 26, 54 and 99, A→G at the sites of 47and 68, and G→A at the site of 63 in the intron 8 of PRLR gene, were detected by cloning, sequencing and homologous comparison of the homozygote AA, BB and CC.

对AA、BB、CC三种纯合子进行克隆测序和同源序列比较，发现在扩增片段内有6处SNP，都发生在PRLR基因的第8内含子，分别是内含子8第26位、54位和99位的C→T突变，47位和68位的A→G突变，63位的G→A突变。

This is represented in diagrammatic form in Fig. 3.15a. If we now consider a belonging to different phones again becomes apparent. Figure 3.15b is merely a simplification of Fig. 3.12. Such interdigitation of fragments of a higher unit is characteristic of all sequencing in speech and language.

若我们现在考虑不同声音间紧密的关系，它们就会再次变得更为明显，图表3.15b仅是图表3.12的简化，这种在较高单位的片段交叉，是说话和语言顺序性的特点。

Previous studies showed that there were many sequencing changes in the genome of offspring introgression lines.

先前的研究表明渐渗系后代基因组中存在大量的序列变异。

Results DNA sequencing confirmed that the nucleotide sequence of synthesized IPSE gene was completely identical to the native one.

结果 人工合成的IPSE基因序列与天然基因序列完全一致。

Methods The IPSE gene of S. man soni was synthesized by overlapping PCR, and confirmed by DNA sequencing. The recombinant plasmid IPSE-pET32a was constructed by inserting the gene of IPSE into expression vector pET32a at the downstream of thioredoxin coding sequence.

应用重叠PCR方法合成曼氏血吸虫IPSE基因，将其定向克隆至表达载体pET32a的硫氧还蛋白序列下游，构建重组表达质粒IPSE/pET32a。

The experiments of treating dyeing wastewater have been finished using filtration with iron filings and Sequencing Batch Reactor method.

该工艺具有投资少、运行费用低、处理效果稳定等特点，尤其适合中小型印染厂的废水治

Many will continue to choose to live in duality and in conflict.

许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现：体育大学的学生乍一看，都是穿运动衣，大家都一样