查询词典 sequencing problem

This technique can be used for observing time sequencing dynamical process of great depth of object field.

这种技术可用于研究大景深物场的时间序列动态过程。

Methods We collected 43 microtia cases' peripheral blood and extract genome DNA. Design primers of three exons of the GSC, then sequence after PCR amplification and depuration sequencing.

采集43例小耳畸形患者的外周血提取基因组DNA，对GSC基因的三个外显子分别设计引物，经PCR扩增纯化后直接测序。

Methods: Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis-ethylene dibromide (PCR-PAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计 12个微卫星多态性标记，采用PCR-中性聚丙烯酰胺凝胶电泳-EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

Methods: Twelve microsatellite markers located at chromosomes 3p, 9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis ethylene dibromide (PCR-FAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计12个微卫星多态性标记，采用PCR-中性聚丙烯酰胺凝胶电泳－EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

Sequence difference between these 2 cell sublines were found in mtDNA D-loop region by sequencing.

序列测定发现，这两个肝癌细胞系在线粒体DNA的D-loop区存在序列差异。

Typical accident-causing" theories includes Heinrich" s Domino" s theory, its development by Bird, Adams and other people, progress of which is that it is deficiencies of management system that are the root cause in accident causation sequence. This is author" s idea resource and main method to produce the water traffic accident-causing theory . in addition, uncontrolled transferring of energy theory, applies widely in safety management scheme for water traffic; human error accident model can be used to analyze causes of collision accident and to take preventive measures; perturbation theory and multilinear event sequencing method is a very useful investigation method to water traffic accidents. All of these theories have contributed to the development of water traffic accident-causing theory. As to orbit intersecting theory, the paper thinks it extension of Domino s theory, although it exists independently.

有代表性的事故致因理论有海因里希因果连锁理论以及博德、亚当斯等人对该理论的发展，这些发展的主要进步是突出了管理缺陷是事故因果连锁中的基本原因，是本文分析和发展水上交通事故致因理论的主要思想来源和方法论；能量意外转移理论，广泛的应用在水上交通安全的各项具体管理制度中；人失误事故模型能用于指导对碰撞事故的原因分析和预防；动态变化理论及多线性事件连锁论对指导事故调查是较好的分析方法；轨迹交叉理论虽然以独立的理论形式存在，但本文认为其实质仍然是因果连锁理论的发展。

Compare the result with that of direct sequencing to draw the conclusion.

基因芯片检测结果与直接测序结果具有很高的符合率。

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

Through transferring, screening, enzymolysis and sequencing, it was demonstrated that this fragment was Laccase gene.

经筛选、酶切、PCR鉴定，序列分析，证明该片断为变色栓菌漆酶基因的克隆。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。