查询词典 sequencing problem

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

In the present study,using PCR amplification and automatic sequencing of EDA gene coding region,we report five mutations identified in 6 Chinese families with XLHED, including three novel ones:p.M1T,p.L62P and

其中3个点突变(p.M1T、p.L62P和p.G195E)为国际首次报道的新突变。

But because of the balky sequencing tools of the day and their high cost, much of the subsequent research progressed slowly or stalled.

但是由于当时测序工具的停滞不前和高成本，很多后续研究进程都十分缓慢甚至停滞不前。

Drosophila Genome Project has been performed by Human Genome Sequencing Center at Baylor College of Medicine.

大鼠基因组计划是由贝勒医学院人类基因组测序中心正在进行的项目。

The topics covered include: cooperative stochastic games; noncooperative stochastic games; sequencing games; games arising form linear infinite programming problems; network formation, costs and potential games; potentials and consistency in transferable utility games; the nucleolus and equilibrium prices; population uncertainty and equilibrium selection; cost sharing; centrality in social networks; extreme points of the core; equilibrium sets of bimatrix games; game theory and the market; and transfer procedures for nontransferable utility games.

包括的题目包括︰合作的随机的比赛； noncooperative随机的比赛；排序比赛；出现的比赛形成线的无限的编程问题；网路阵式，费用和潜在的比赛；用可转让的效用游戏的潜能和一致；核仁和均衡价格；人口不确定和均衡选择；费用分享；在社群网里的centrality；核心的极端点；均衡bimatrix 比赛；运筹学和市场；并且转移不可过户的效用比赛的程式。

Objective To establish DNA sequencing method for the identification of ABO blood group.

目的 通过序列测定方法对ABO血型进行深入研究。

DNA is the carrier of genetic information and sequencing and comparison of DNA nucleotides are the thorough and ideal method for bryophyte molecular phylogenesis.

DNA是遗传信息的载体，直接对DNA核苷酸排列顺序的分析和比较是研究苔藓分子系统学的最彻底和最理想的方法。

Barrels Sorting Algorithm : This is a bubble sort than better performance, but require more memory sequencing methods.

桶排序算法：这是一种比冒泡排序有更好性能，但是要求更多内存的排序方法。

This design develops the Firsthuida material management system ,the system will be able to achieve the company's demand, and urge the material supervisory work achieve the company standardized, the sequencing, and avoided the material management capriciousness, enhanced the speed and the accuracy of the information processing, then employee could prompt, accurate, effective inquiry and revises the material situation.

本设计所开发的第一会达公司资料管理系统正迎合了公司的需求，资料管理系统地实现将会使公司的资料管理工作更加规范化、程序化，避免资料管理的随意性，提高信息处理的速度和准确性，能够及时、准确、有效的查询和修改资料的情况。

Capsid proteins are involved in recognition and infection of the host and can be used to identify the virus by serological means or amino acid sequencing.

衣壳蛋白与寄主的识别和感染有关，还可以通过血清学的方法或氨基酸序列来识别病毒。

Many will continue to choose to live in duality and in conflict.

许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现：体育大学的学生乍一看，都是穿运动衣，大家都一样