查询词典 sequencing problem

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

Now more and more vertebrate genome sequencing tasks have been completed, such as chicken, dog, cattle, chimpanzee and fugu fish.

目前已经有鸡、犬、牛、黑猩猩、河豚等越来越多的脊椎动物基因组测序任务完成。

Bioinformatics methods are used to analyze and manage expressed sequence tags which are generated by large-scale sequencing of a cDNA library from cephalothorax of a wild, female, adult Chinese shrimp.

本文运用生物信息学的方法，分析及管理由雌性成年中国对虾头胸部cDNA文库经部分测序产生的10446条ESTs。

The gene of ESAT-6 from genome of bovine mycobacterium tuberculosis strain that from cervine tuberculosis was amplified by polymerase chain reaction and inserted into pGEM-TE vector. After sequencing, we analyzed the gene encoding ESAT-6 protein with molecular biologic software.

从患结核病鹿的肺组织中粗分离牛分支杆菌制备DNA模板，用PCR对牛分支杆菌ESAT-6基因进行了扩增，将扩增的产物连接于测序载体pGEM-TE上克隆，经测序确定无误；并使用分子生物学软件对ESAT-6基因进行了分析。

Chaparral Hall includes 18 research labs, nine introductory teaching labs and 13 lecture rooms in addition to a DNA sequencing lab and a microscopy suite.

丛林科学馆包含18个研究实验室，9个教学实验室和13个演讲室，另外还有一个DNA实验室和显微镜套件实验室。

Disorganized process of thinking characterized by disruption of goal-directed sequencing, e.g., circumstantiality, tangentiality, loose associations, non-sequiturs, gross illogicality, or thought block.

概念紊乱指思维过程紊乱，表现为目标导向次序混乱，如病理性赘述、离题、联想散漫、不合前提的推断、整体缺乏逻辑或思维中断。

The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.

从7种重要鲽形目经济鱼类——高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽和条鳎、夏鲆的脑垂体中提取mRNA，并采用RT-PCR方法，根据已克隆得到的鲆鲽鱼类生长激素基因的保守性序列设计特异性引物，克隆了含开放阅读框的生长激素(Growth Hormone，GH)cDNA序列，测序结果显示，高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽、条鳎和夏鲆的GH cDNA长度依次为479 bp、564 bp、519 bp、440 bp、564 bp、440 bp和522 bp，编码140-170个氨基酸的成熟GH多肽片段；从漠斑牙鲆和大菱鲆脑垂体中提取mRNA，利用SMART-RACE技术建立脑垂体cDNA文库，并从该文库中克隆出大菱鲆和漠斑牙鲆生长激素基因cDNA全长序列。

According to the results of sequencing, lactic acid bacteria and Clostridium sp.

UPGMA聚类分析和PCA分析结果也表明，pH=7和pH=8时的微生物群落结构相似，pH=5与之显著不同。

This study adopts PCR method of coagulase - and sequencing RouGe detection a fragment of gene ESR polymorphism, and its single-nucleotide polymorphisms egg and correlation analysis, explore properties with different genotypes RouGe egg performance, from the relationship between genetic level RouGe reveal candidate function mechanism of molecular genetics of egg performance, RouGe new strains of selection for the use of genetic material.

本研究采用PCR-SSCP和测序的方法检测肉鸽ESR基因某一片段的多态性，并将其单核苷酸多态性与产蛋性能进行相关性分析，探索不同基因型与肉鸽产蛋性能之间的关系，旨在从候选功能基因层次揭示肉鸽产蛋性能分子遗传机理，为肉鸽新品系的选育提供可借鉴的遗传资料。

Wang is the ultimate CD-omnipotent form a concise, and very low occupancy resources function is not comparable to similar green multimedia player, it is simple, complete functions, supports a wide range of audio and video formats, and can be adjusted at will broadcast pace and sequencing.

终极影音全能王是一款外形简洁，占用资源极低而功能却不逊于同类的绿色多媒体播放程序，它操作简便、功能齐全，支持多种音频和视频格式，且能够随意调整播放进度和顺序。

Many will continue to choose to live in duality and in conflict.

许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现：体育大学的学生乍一看，都是穿运动衣，大家都一样