查询词典 sequencing problem

Strict and perfect system of quality control in DNA sequencing and sequence analysis is the guarantee for exact and reliable results.

在大规模序列测定和分析过程中严格健全的质量控制体系是获得准确、可靠结果的保证。

MAIN OUTCOME MEASURES: The results of recombinant plasmid PCR and gene sequencing, the correlation coefficient of the standard curve and the repeatability of the plasmid standard.

主要观察指标：重组质粒聚合酶链反应和基因测序结果，标准品标准曲线的相关系数及标准品的重复性。

Polymorphism was detected for exon 3 of BMP4 gene in four sheep breeds. For exon 3, three genotypes were detected in four sheep breeds. In Small Tail Han, Chinese Merino, Corriedale and South African Mutton Merino sheep, frequency of genotype AA was 0.017, 0.216, 0.115, 0.429, frequency of genotype AB was 0.102, 0.317, 0.269, 0.500, frequency of genotype BB was 0.881, 0.467, 0.616, 0.071, respectively. Sequencing revealed one singlenucleotide mutation C→A at 305 bp of exon 3 of BMP4 gene in genotype BB in comparison to genotype AA, and this mutation resulted in an amino acid change of alanine→aspartic acid.

对于外显子3的引物，在4个绵羊品种中都检测到3种基因型；统计结果表明：小尾寒羊、中国美利奴绵羊、考力代绵羊、南非肉用美利奴绵羊AA基因型频率分别为0.017、0.216、0.115、0.429，AB基因型频率分别为0.102、0.317、0.269、0.500，BB基因型频率分别为0.881、0.467、0.616、0.071；测序结果表明：BB型和AA型相比在BMP4基因外显子3第305位碱基处发生C→A的突变，该突变导致氨基酸由丙氨酸改变为天冬氨酸；BB型小尾寒羊平均产羔数分别比AB型和AA型多0.61只（P.05）和1.01只（P.05）。

In order to understand the prevalence of conditions of the disease in Shandong Province, 6 strains of bacteria were isolated from 20 suspected Infectious Coryza chickens in vivo from five farms in Dezhou region of Shandong Province, and identified as Haemophilus paragallinarum by the bacterial culturing, biochemical tests sequencing of 16S rDNA gene, chicken embryo inoculation test.

为了了解该病在山东省的流行状况，从山东德州地区5个鸡场的20只疑似鸡传染性鼻炎鸡体内共分离到6株细菌，经细菌分离培养、生化试验、鸡胚接种试验和16s rDNA基因序列分析等方法鉴定为副鸡嗜血杆菌。

By determining the individual effects of configurations and material properties through modeling, a 3-D sequencing map covering the span of compliance, Poisson's ratio, and piezomagnetic coefficient ratio of the magnetostrictive phases is developed to predict the homogenized M-E voltage coefficient,, curves.

在利用等效磁电参数 a 量化了各个配置的磁电耦合效应后，我们发展了一个涵盖了全范围磁致材料的 compliance 、 poisson's ratio 、以及 piezomagnetic coefficient ratio 等材料性质的万用排序空间图。

Pepper ; cucumber mosaic virus ; CP gene ; 2b gene ; sequencing

辣椒；黄瓜花叶病毒； CP基因； 2b基因；序列分析

By sequencing randomly-selected clones from the library and usingbioinformatics, 73 novel expressed sequence tags were obtained, in which therewere 24 full-length cDNAs, including cytolysin gene, neurotoxin genes, and greenfluorescence gene. Functional study on these novel genes is an ongoing task in ourlaboratory.

对文库克隆进行序列测定和生物信息学分析，已经获得了73个海葵新表达序列标签，有24个全长cDNA，其中包括海葵细胞毒素、海葵神经毒素、绿色荧光蛋白等具有开发应用价值的新基因。

Acceptance Criteria Activity Duration Estimation; Activity Definition; Activity Sequencing; Addenda Approved budget Arbitration Award of Contract ; Award Criteria Audits Balanced Matrix; Baseline Bill of Quantities Brainstorming Characteristic Checklists civil engineering civil building Claims Clarify Closing Communication Management Communications Planning Communications Skills Communication channels or paths Completion Computer-based Concrete structure Conditions of Contract Conflict Management Contract Management Contract administration Change Control Contract Closure Contract file Contract agreement; Contractual obligations Contractor Contractor's Representative Construction Management Planning Contract Price Control Chart Cost Estimating; Cost of Quality Cost Control Cost Budgeting Cost Management Co-operation Failure costs Critical Path Feedback Fixed-price Fossils Force Majeure Daywork Functional Organization Decision; Fundamental Tenets Decision Tree Defects Liability Definitions Delphi technique General Rules Deliverables General Provisions Deviation; Generation Direct Costs Discounted cash flow; Dissemination Highway engineering; Discharge Human Resource Management; Disputes Human Resource Planning Drawings Hydraulic engineering; Hydroelectric engineering; Employer's Taking Over Identity Certificate Evaluation of Tenders Initiating Process Group; Executing Process Group Instructions to Tenderers Industrial building Investment payback analysis; Ineffective Individual and team performance Information Distribution Negotiation Indirect Costs Nominated Subcontractors Incentive bonus Information Technology Interpretation; Insurance Observation Inspections Off-site Online On-the-job training Opening of Tender

验收标准活动时间的估计；活动的定义；活动序列分析；增编核定预算仲裁授予合同；授标标准审计平衡矩阵；基线工程量清单集思广益特性清单土木工程民用建筑索赔澄清闭幕通信管理通信规划沟通技巧沟通渠道或路径完成以计算机为基础的混凝土结构合同条件冲突管理合同管理合同管理变更控制合同结束合同文件合同协议；合同义务承包承包商的代表规划建设管理合同价格控制图成本估算；质量成本成本控制成本预算成本管理合作失败成本关键路径反馈固定价格化石不可抗力计日工职能组织决定；基本原理决策树缺陷责任定义德尔福技术的一般规则交付的一般规定偏差；代直接费用贴现现金流量；传播公路工程；放电的人力资源管理；争端的人力资源规划图纸水利工程；水电工程；雇主接管身份证书评标启动过程组；执行过程组指示的投标工业建设投资回报分析；无效个人和团队的业绩信息分配谈判指定分包商的间接费用激励奖金信息技术解释；保险观察视察场外在线在职培训开幕投标

RESULTS: We cloned cytosine deaminase gene and the results from gene sequencing were the same as Genbank.

将胞嘧啶脱氨酶基因亚克隆到pIRES2-AcGFP1质粒上，构建了pIRES2-AcGFP1-CD真核表达载体。

Sequencing refers to the delineation of the order of the repeating units in DNA and protein.

测序是指描述DNA或蛋白质中重复单位的序列。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。