查询词典 sequencing problem

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

Our patient evidenced a wide range of misidentification problems which mainly consisted of misidentifying place, person, and event, and these problems seem to be associated with deficits of temporal sequencing of memory, visual-spatial and visual perception, executive function, and awareness.

这位病患呈现多种层面的错认症状，这些症状可能与病患的时间顺序记忆、视空间知觉、执行功能与缺乏病识感等，多重认知功能缺损有关。

In this study, a stress induction expression promoter of monocotyledon (P-WCS120) was amplified from wheat genome. The result of sequencing displayed a base substitution mutant of 1 bp and an insertion mutant of 21 bps. This sequence was registered at GENEBANK as a new found sequence, because 3% of the sequence was found to be non-homologous with the original sequence.

本研究从小麦基因组扩增单子叶植物逆境诱导表达启动子P-WCS120，测序证明包括1个碱基的替代突变和21个碱基的插入突变，与原序列有3%的非同源性，被GENBANK认定为新发现序列，现已登记注册。

Cloning and sequencing study of human bone morphogenetic protein 7 mature peptide ［J］.

人骨形成蛋白7成熟肽cDNA的克隆和序列测定［J］。

Cloning and sequencing study of human bone morphogenetic protein????

人骨形成蛋白7成熟肽cDNA的克隆和序列测定［J］。

Objective To analyse the cloning and sequencing of mature fragment of human bone morphogenetic protein－4 gene.

目的研究人骨形态发生蛋白4(2b)(BMP-4)成熟区cDNA的克隆方法，并进行测序及序列分析。

The Brownian network which approximates a two－station multiclass queueing network with dynamic scheduling capability is extended to the case of multistation multiclass manufacturing systems and the appropriate algorithms regarding the input control and sequencing decisions are also developed.

2对于一般结构的制造系统，首次建立了可靠分析的一种线性多变量模型，模型阶数为工作站个数，模型变量为各工作站的实际平均加工时间，由此求得了系统的包括可靠性的稳态运行性能，解决了一般结构的制造系统的性能评估问题。

At the end of July, scientists at the Max Planck Institute in Leipzig announced one of the most intriguing breakthroughs of modern science. Using sophisticated sequencing techniques, they had discovered it would be possible - at least in theory - to piece together fragments of DNA preserved in ancient bones and thereby construct a li ving, breathing r eplica of Neanderthal man.

今年7月末，在德国莱比锡马普研究所，那里的科学家宣布了现代科学中最吸引人的突破之一：他们发现，至少在理论上，他们可以利用复杂的基因序列技术，把保存在古代人骨中的DNA片段拼接起来，从而复制一个有生命、很逼真的尼安德特人。

By using RT-PCR method, partial sequences of ppo genes were amplified from cDNA of Nelumbo nucifera's young leaves. After cloning and sequencing, 7 sequences ranging from 978bp to 1057bp were obtained, and they encoded 326 to 352 amino acids.

用RT-PCR方法，从中国莲幼叶RNA中成功扩增出ppo的部分序列，经克隆测序，得到长度在978~1057bP的序列共7个，编码326~352个氨基酸。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。