查询词典 sequenced

Chemnitzi and Pteria penguin using PCR technique. The PCR products were directly sequenced, and 439 bp nucleotide sequences were obtained after excluding the primers and truncating partial sequences at the end.

chemnitzi和企鹅珍珠贝的16S rRNA基因片段，PCR产物直接测序，删去引物及部分端部序列后，得到439 bp可供分析的核苷酸片段，用MEGA 3.1软件分析了核苷酸差异。

The provirus DNA was extracted and amplified by nested-PCR, after which the HIV-1 membrane protein gene C2-V3 region was sequenced and its subtype was analyzed.

艾滋病病毒是一种具有高度变异性的逆转录病毒，根据血清学反应的不同，可将其分为HIV-1和HIV-2两种型别。

Provirus DNA was extracted, HIV-1 gag genes were amplified by nested-PCR and sequenced directly with pairs of specifically designed primers, sequences were then analyzed by GCG software system to determine genotypes, and phylogenetic trees were established.

采集17名感染HIV的农村外来妇女及这些外来妇女的4例阳性配偶的外周血，提取前病毒DNA，应用套式PCR进行艾滋病病毒gag基因检测，使用GCG软件包对扩增序列进行分析，并对这些HIV感染者进行相关流行病学调查。

In a summary,in this study,the entire provirus genomes of EIAV L and DLA-EIAVprovirus were cloned and sequenced.Speculation about the function and structure of EIAVgenome was drawn upon the comparison of EIAV L,DA-EIAV,DLA-EIAV and other alienEIAV strains.

总之，本研究首次获得了我国DLA-EIAV和L株前病毒全基因克隆，确定了DLA-EIAV和L株前病毒基因组核苷酸全序列，并对EIAV L株、DA-EIAV、DLA-EIAV及国外相关的EIAV毒株进行了比较分析。

We collected the whole blood from 25 anti - HIV- 1 antibody positive cases and separated the peripheral bloodmononuclear cells. The provirus DNA was extracted and amplified by nested- PCR, after which the HIV - 1 mem-brane protein gene C2 - V3 region was sequenced and its subtype was analyzed.

采集了25份HIV-1抗体阳性感染者的全血分离单核细胞，提取前病毒DNA，经nested-PCR扩增HIV-1膜蛋白基因的C2-V3区并进行序列测定和亚型分析。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

HIV proviral DNA present in the peripheral blood mononuclear cells from the patient and his wife was amplified by a nested polymerase chain reaction and sequenced directly.

艾滋病毒基因存在于外周血单个核细胞，从病人和他的妻子被扩增一聚合酶链反应和直接测序。

HIV proviral DNA present in the peripheral blood mononuclear cells from the patient and his wife was amplified by a nested polymerase chain reaction and sequenced directly.

艾滋病毒基因存在于外周血单个核细胞，从病人和他的妻子被扩增一聚合酶链反响和直接测序。

We sequenced the whole genome of the proband, identified all potential functional variants in genes likely to be related to the disease, and genotyped these variants in the affected family members.

我们对该病的先证者进行全基因测序，鉴定所有可能与该病相关的功能性变异基因，并确定在受累家族成员中这些变异基因的基因型。

When this condition occurs, inbound replication with the source partner is stopped on the destination domain controller and event ID 2042 is logged in the Directory Services event log.

计算机密码学是研究计算机信息加密、解密及其变换的科学，是数学和计算机的交义学科，也是一门新兴的学科。

Instructions: click on the thumbnails to see a larger image, then use the left-right arrow keys to scroll through the slideshow.

使用说明：滑鼠点在小图上即可放大观赏。开启后键盘左右键可用来换照片。