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We amplified and sequenced TSER in different primates, including hominoids; two subfamilies of Old World monkeys : colobines and cercopithecines; and two species of New World monkeys . Besides in humans, TSER length difference polymorphisms were also found in some primate populations, although in other primate populations, this region has been fixed.

灵长类TSER的进化为了弄清TSER重复结构进化，我们研究了包括人猿超科、猴科的两个亚科疣猴亚科和猴亚科及卷尾猴科的共32个物种的TSER序列变异情况。

The results were as follows:①Introns of sheep myogeningene were firstly cloned and sequenced.

研究结果表明：①首次成功地克隆了羊的肌细胞生成素基因的全部内含子。

Separated cultures were isolated from 4 different koumiss examples, the complete 16SrRNA gene of these strains were sequenced, and phylogenetic trees were constructed. The isolateswere identified to the most related strain which had the highest affinity. The identified strains weresix L. lactis, five L. plantarum, five E. faecium, four E. faecalis, two L. casei, two L. helveticus, twoS. thermophilus, one L. delbrueckii subsp, bulgaricus, one Leu. mesenteroides, one Leu. garlicum.

本研究中从4份酸马奶中分离得到了29株乳酸菌，测定了这些乳酸菌的16S rRNA基因全长序列，并构建了乳酸菌属的系统发育树，根据亲缘关系对乳酸菌进行了鉴定，其中L.lactis(6株)，L.plantarum(5株)，E.faecium(5株)，E.faecalis(4株)，L.casei(2株)，L.helveticus(2株)，S.thermophilus(2株)，L.delbrueckii subsp.bulgaricus(1株)，Leu.mesenteroides(1株)，Leu.garlicum(1株)。

In this paper, we applied PCR-SSCP technology to detect the gene mutation of IGF-I gene in New Yangzhou chickens(130), Xueshan chickens(100) and White Leghorns chickens(47) and DNA sequenced.

建立统计分析模型，应用GLM过程对新扬州鸡部分生长和屠宰性状进行分析。本论文得到的结论如下： 1。

Total RNA was isolated from phloem of young shoots and retro-transcripted to cDNA by RT-PCR with specific primers, and the amplified cDNA fragments were sequenced for identification of subtypes. An effective RT-PCR technique was established for detecting citrus viroid types, and three types were recognized, i.e.

以田间柑橘枝条韧皮部为试材，提取总RNA，反转录成cDNA，再用不同类型类病毒的特异引物进行PCR扩增，鉴定出各个样品所携带的柑橘类病毒类型；通过测序分析各类型的序列，并与国内外报道的序列进行同源性分析，确定所测样品的亚型。

A uricase gene was cloned and sequenced from Bacillus subtilis strain CCRC 14199. The cloned uricase gene contained an open reading frame of 1491 nucleotides encoding a protein of about 55 kDa. A uricase-MBP fusion protein of about 98 kDa was expressed and purified.

尿酸氧化酵素基因是来自枯草杆菌CCRC14199的重组DNA，其基因序列包含1491 核甘酸约55 kDa蛋白质，及表现在麦芽糖结合蛋白大约98 kDa之融合蛋白质，并呈现高尿酸氧化酵素的活性(9.1 U/mg)。

The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^＋ and Leu2^＋LacZ^＋ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法扩增Id1的编码序列并定向克隆入诱饵质粒pHybLex/Zeo，构建重组诱饵质粒pHybLex/Zeo-Id1，酶切鉴定后转化入酵母株EGY48/pSH18-34，检测重组诱饵质粒有无非特异激活报告基因Leu2、LacZ作用；扩增并提取成人肺组织文库质粒，将文库质粒及重组诱饵质粒转化入酵母细胞，依次筛选Leu2^＋，Leu2^＋LacZ^＋克隆；设置阴性及阳性对照，重复筛选Leu2＋LacZ＋克隆并排除假阳性克隆，获取真阳性文库质粒，进行测序和同源性比对。

Results Fifty-one clones screened from 160 clones on the basis of Hae III digestion patterns were sequenced, and their sequences were deposited in the GenBank. Clone sequences (52.9%) belonged to Acidimicrobidae and 5 suborders of Actinobacteridae. The other clone sequences (47.1%), which formed one large distinct clade in phylogenetic tree among phylum Actinobacteria, may represent one new suborder or new class.

所获得的51个克隆序列属于39个OTUs，其中52.9%的克隆序列分布于放线菌门放线菌亚的5个亚目和酸微菌亚纲中，并且在这两个亚纲中有大量克隆序列属于放线菌的新类群，另外47.1%的克隆序列以极高的自展值在放线菌门内支持形成一个独立的大分支，极有可能代表一个新亚目或更高级分类单元的类群。

Time-sequenced images have recently shown that spicules last about five minutes, starting out as tall tubes of rapidly rising gas but eventually fading as the gas peaks and falls back down to the Sun .

一时间为顺序的图像最近显示出了这耀斑只持续了5分钟，以渐渐升起的气体云的形态出现但逐渐下落最后落回太阳表面。

Time-sequenced images have recently shown that spicules last about five minutes, starting out as tall tubes of rapidly rising gas but eventually fading as the gas peaks and falls back down to the Sun .

最近依时间序列得到的图像表明，针状物大约持续了五分钟，始于迅速上升的强大气体漩涡，最终随着气体抛射达到高峰并回落到太阳表面而衰落。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。