查询词典 sequenced

Then we can pre-empt problems by building the mold with special inserts to rotate the flows into double-stacked hot-runner manifolds through sequenced valve gates into pressure-monitored cavities fabricatedfrom a myriad of steels coated with exotic super-hard, lubricious, rust-proof platings applied with little heat and thinner than air.

然后，我们捷足先登：用特殊的嵌件构建模具，使料流旋转进入双层结构的热流道，再通过顺序浇口的开启注入到压力监控的模腔，这些模腔是用涂覆着超硬的、润滑的、防锈的电镀层制造的，这些电镀层绝热效果好，比空气分子还薄。

The clone plasmids of the 3 genes were extracted, then analysed with restriction enzyme and sequenced respectively.

提取克隆菌落的质粒，并进行酶切鉴定和序列测定。

All the recombined suicide plasmids were successfully obtained and sequenced, and 24 unique sequences were identified. Bioinformatics analysis showed that there were 51 ORFs in the 24 operons.

对插入的随机片段进行测序后进行生物信息学分析，共证实有24个不同的基因片段，在TIGR4全基因组里面，这24个基因片段所处的操纵元结构中，总共包括51个开放阅读框，其中多数为含有多个ORFs的操纵元结构。

In our former study, we cataloged genes expressed in human brain (about 10,000 gnes), and sequenced the orthologous sequence of these genes in chimpazee. In this study, we selected 16 of these positive-selection genes and then using PCR and Sequencing technique we acquired orthologous sequence in another two representative primates: white-brown ape and macaca, and then took further evolutionary analysis.

本文从目前已知在人的大脑中表达的基因中，选出16 个在人和黑猩猩的比较中明显受到正向选择的基因，利用PCR扩增技术和基因序列测定技术，获得了另外两个灵长类代表动物小猿、猕猴的直系同源序列，并进行了进化分析。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

The samples of H5N1 virus, taken from the first two victims who died of bird flu in Turkey, were sequenced at a World Health Organisation collaborating centre at the National Institute for Medical Research in London, UK.

H5N1 病毒的样本是从土耳其死于禽流感的最初两位受害者体内提取的，由世界卫生组织设在英国国家医学研究院的协作中心检测其序列的。

Methods Firstly, total DNA in the root tuber of P. multiflorum from various habitats was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and sequenced after purification.

从来自不同原产地的何首乌中提取总DNA，以核基因组ITS通用引物为引物进行扩增，扩增产物经纯化后，用PCR产物直接测序法进行测序。

Mothods: Cock embryo was used to isolate influenza viruses, and the virus titer was determined through hemagglutination test. The gene was amplified by RT-PCR technique from the isolated strain and sequenced, and the gene phylogenetic tree was analyzed.

采用鸡胚进行病毒分离，采用血凝试验初步确定病毒效价，用逆转录聚合酶链反应法扩增分离株的部分片段后进行克隆和测序，然后进行进化树分析。

Around 120 serum samples were collected from health care workers and other contacts of the infected boy and test results should be available early in the week beginning 12 January. The H9N2 virus isolated from the boy in December 2003 has been sequenced by the National Influenza Centre at the Governmental Virus Unit, Hong Kong SAR and antigenically characterized by WHO Collaborating Centres.

从患者的医护人员及其他接触人群中采集了大约120份的血清样本进行检验，检验结果将于下周初（1月12日）得出。2003年12月从患儿身上分离出的H9N2病毒由设在香港特区政府病毒组的国家流感中心进行了基因排序，世界卫生组织协作中心进行了抗原特性检验。

To study the genetic diversity and evolution of four breeds of Fujian domestic ducks, the 667 bp control region of mitochondrial DNA from 32 individual domestic ducks and eight spot-billed ducks were sequenced. The results showed that in the D-loop region of four domestic duck breeds, the content of A, G, C and T nucleotides was 25.5%, 15.2%, 33.5% and 25.8%, respectively.

为了探讨福建省家鸭的遗传多样性和品种进化，本研究测定了福建省4个地方家鸭品种的32个个体和野生斑嘴鸭8个个体的线粒体DNA D-loop区667bp序列，分析了这4个家鸭品种的遗传多态性及品种进化。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。