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The most pathogenic isolate, VIB 645, contained two closely related hemolysin genes, which were cloned and sequenced. In this study, vhhA was cloned into an expression plasmid pET-24d.

本研究将哈维氏弧菌的溶血素基因vhhA克隆到大肠杆菌的表达载体pET-24d，VHH溶血素作为一种带六个组氨酸的融合蛋白在大肠杆菌表达菌株BL21(DE3)中得到了过量表达，重组大肠杆菌及其培养上清液在鱼血平板上有很强的溶血活性。

Results Five of the PCR amplifications were discovered and sequenced with 300 bp ...

结果对 5个阳性标本的 30 0bp部分M基因组片段序列分析表明均为SEO型汉坦病毒；毒株核苷酸序列比较表明：与其他SEO型毒株同源性均达 94%以上，而与其他HNT型毒株同源性均低于 72 %；毒株序列所构建的系统发生树显示，北京地区SEO型汉坦病毒可能至少存在 4个不同的进化分支和两个不同的汉坦病毒亚型。

Ten alleles sequenced had the same modular structure, starting with a repeat block of Type 3 followed by a block of Type 1 before the central block of Type 3 (313) at 5'end, and starting with a repeat block of Type 4 followed by the central repeat block of Type 3 (43) at 3'end.

另一种为新发现的变异核心序列，暂命名为7型（type 7）。10个等位基因具有相同的模块结构，在5'端表现为：3型一1型--3型（313）排列，在3'端则表现为：4型一3型（43）排列。

The full-length FKN gene of human, chimpanzee, gorilla, orangutan, gibbon, macaque and leaf monkey were sequenced successfully, and the FKN sequences analysis lays the foundation for further studying its evolution in immunological function in higher primates and the relation between its structure and function.

成功获得人、黑猩猩、大猩猩、红毛猩猩、长臂猿、猕猴和叶猴7种高等灵长类物种FKN全基因序列，为后续探讨FKN在高等灵长类物种进化过程中免疫学功能演变及其结构与功能的关系奠定基础。

Each of inserted DNA fragment in these recombinants was sequenced to verify the DNA sequence of mutants cloned.

每个克隆重组子中插入的目的基因进行DNA测序以验证基因突变型。

Results The sequenced segment (590 bp)obtained in the recombinants was identical to that reported by GenBank and the segment inserted in the correct direction.

结果 克隆获得的590 bp的DNA序列与GenBank报道的一致，且插入方向正确。

Results: The sequenced segment （1 175 bp） in the recombinants was identical to that GenBank had reported and the segment was inserted in right direction.

结果：序列测定表明，克隆获得的1175bp的DNA序列与GenBank报道的一致，且插入方向正确。

Four repeatably differential expression fragments were reclaimed from the polyacrylamide gels, re-amplified, cloned and sequenced.

获得5对能够扩增出5条对小麦抗叶锈病近等基因系TcLr41具有特异性条带的引物，分别是P-AC/M-CAA、P-AG/M-CAT、P-AG/M-CCC、P-CC/M-CCA、P-GA/M-CAA。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N末端带上含24 bp的flag标签，克隆到pGEMT easy载体并测序，再亚克隆至真核表达载体pcDNA31，酶切鉴定正确后采用脂质体法瞬时转染COS7细胞，Western blot检测flagcbl在细胞中的表达。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。