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Six days after inoculation, the cucumber root tip was slightly swollen and symmetrical root-knots were observed at the meristematic and elongation zones. Giant cells were differentiated by previous cambium cells in the center of the root; 10days after inoculation, symmetrical and secondary root-knots were observed at the root hair and upper zones. Vessels were separated by giant cells, around which protoxylem vessels were observed; l6days after inoculation, hypo-vascular tissue appeared earlier than normal; 20-30 days after inoculation, the vertical arrangement of vessels was disordered, shape was distorted and a new vascular tissue appeared in the cortex.

结果显示:接种后6 d的根尖分生区、伸长区出现膨大、对称的根结,根中心的原形成层细胞分化出巨型细胞;10 d在根毛区及以上部位出现既有对称又有偏于一侧的根结,周围有原生木质部导管出现,根结中巨型细胞阻断了导管;16 d次生维管组织提早出现;20~30 d导管纵向排列无序,形状扭曲,皮层中有新维管组织产生。

A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus.

皮层在发育早期以及纹状体和海马CA区在发育各期检测到极少数为新生神经元细胞,多数分化为胶质细胞;海马齿状回以神经元细胞再生为主;胶质细胞的再生随脑组织发育的成熟而逐渐减少。

AIM: To study the effects of basic fibrolast growth factor on the proliferation of lymphokine activated killer cells from patients with bladder cancer and LAK cells cytolysis against bladder tumor cells.

目的:探讨白细胞介素Ⅱ(IL2)和碱性成纤维细胞生长因子对膀胱癌患者淋巴因子激活的杀伤细胞的作用。

In winter,nuclear of GTH cells were elliptical and a small number of secretory granules scattered around nucleolus. Number of secretory granules in STH cells were relatively small, nucleolus distorted, activity of synthesis and secretion decreased. Secretory granules in TSH cells cytoplast were relatively small.

冬季,GTH细胞核为椭圆形,有少量的分泌颗粒散布在细胞核的周围;STH细胞分泌颗粒数量较少,胞核变形,合成和分泌活动比较微弱;TSH细胞胞质中的分泌颗粒相当多。

In winter,nuclear of GTH cells were elliptical and a small number o f secretory granules scattered around nucleolus. Number of secretory granules in STH cells were relatively small, nucleolus distorted, activity of synthesis and secretion decreased. Secretory granules in TSH cells cytoplast were relatively small.

冬季,GTH细胞核为椭圆形,有少量的分泌颗粒散布在细胞核的周围;STH细胞分泌颗粒数量较少,胞核变形,合成和分泌活动比较微弱;TSH细胞胞质中的分泌颗粒相当多。

Numbers of secretory granules, oplasmic reticulums, mitochondrias in ACTH cells all changed distinctly. GTH cytoplast were full of big and small, high density electron secretory granules.STH cells were in activity synthesis and secretion, secretory granules were from fusion to release. TSH cells cytoplast evacuated secretory granules, electron density decreased and vacuofes appeared.

ACTH细胞分泌物颗粒数量、内质网及线粒体均发生明显变化;GTH细胞胞质中充满电子密度较高的大、小两种分泌颗粒;STH细胞处于活跃的合成和分泌状态,分泌颗粒呈现出由融合到释放的状态;TSH细胞胞质中分泌颗粒排空,且电子密度降低,出现大空泡。

Methods: The Hela cells were cultured in vitro and treated with different concentrations(0.125~2.0 mg/ml) of matrine solution for 24~72 hours. MTT assay was performed to evaluate the cytotoxin effect of matrine on Hela cells. FCM was used to detect the apoptosis of Hela cells. Transmission Electron Microscope analyses were carried out to examine the cell morphology.

选用人宫颈癌Hela细胞进行体外培养,以0.125~2.0 mg/ml的苦参碱分别处理Hela细胞24~72 h后,MTT法检测细胞增殖,流式细胞仪检测细胞凋亡率,透射电镜进行形态学观察,RT-PCR半定量检测凋亡相关基因Bcl-2和Bax的mRNA表达水平。

With cells 7404 as the target cells, the killer role of rIL-2 and cytotoxin of LAK cells in the case of pH 6.8, 7.3 and 7.6 will be assayed one by one by the MTT method.

应用细胞计数方法观察pH值分别为6.8、7.3、7.6的培养液,对rIL-2激活单个核细胞的影响。

With cells 7404 as the target cells, the killer role of rIL-2 and cytotoxin of LAK cells in the case of pH 6.8, 7.3 and 7.6 will be a ayed one by one by the MTT method.

应用细胞计数方法观察pH值分别为6.8、7.3、7.6的培养液,对rIL-2激活单个核细胞的影响。

There are two disputing theories about the origin of cancer, that is, cancer arises from dedifferentiation of somatic cells or maturation of stem cells. Recent discoveries demonstrate that cancer cell proliferation is driven by cancer stem cells.

肿瘤的起源,即肿瘤的去分化起源和肿瘤的干细胞起源一直是有争议的,而随着干细胞研究的深入,越来越多的实验结果证实肿瘤起源于干细胞的观点。

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