查询词典 insert cells

Because these viruses abortively infect human and most mamralian cells yet still effecfivelypresent encoded forgein genes to the host inmune system Compared with the replication-compentent vaccinin virus,the MVA modified vaccinia Ankara ,all a highly attenuated vaccinia virus,lost hte ability to replicate in human and most mamralian cells except BHK-21 and CEF cells.

痘苗病毒做为一种载体在外源抗原蛋白的递呈方面有着很大的潜力。因为这类病毒不能感染人和大多数哺乳动物细胞，却可以有效地将外源基因编码的蛋白递呈给宿主免疫系统。

Results:The results show that the survival rate of mammary epithelial cells were decreasing gradually with the increasing of the time of TGF-β1 treatment, and the survival rates of 24 h and 48 h groups were significantly lower than that of control group (p.05); the LDH activity of 6 h、12 h、24 h and 48 h groups were significantly higher than that of control group (p.05); the DNA degradation arisesed in 12 h, 24 h, and 48 h groups' mammary epithelial cells; the activity of Caspase-3 reach to a peak at 24 h, which was significantly higher than that of control group (p.05); the acinus of epithelial cells appears to break off and collapse with the increasing of treatment time, but the leydig structure keeps integrate;...

结果表明：1、5、10 ng/mL的TGF-β1均能抑制乳腺上皮细胞的增殖，且随浓度增加抑制作用加大，呈现剂量依赖性，其中10 ng/mL TGF-β1组与对照组差异显著（P.05）；10 ng/mL TGF-β1作用于乳腺上皮细胞24 h，随作用时间延长细胞凋亡率逐渐增加，6 h、12 h和24 h组与对照组相比差异极显著（P.01）；LDH活力也不断升高，6 h、12 h和24 h组与对照组差异显著（P.05）；DNA随 TGF-β1作用时间的延长发生不同程度降解；TGF-β1作用2 h~6 h细胞大量表达HSP70，6 h达到高峰，而后下降，2~12 h的表达量均显著高于对照组（P.05）。结论：TGF-β1可以抑制奶牛乳腺上皮细胞的增殖，具有浓度依赖性。10 ng/mLTGF-β1。。。

The results showed that the glandular epithelial cells of serous acinus,the epithelial cells of all gland ducts and parasympathetic ganglion cells appeared GnRH positive in immunoreaction.The positive substance was distributed in the cytoplasma with negative nuclei.

结果显示，大鼠颌下腺的浆液性腺泡的上皮细胞，各级导管的上皮细胞及副交感神经节细胞均呈促性腺激素释放激素免疫反应阳性，阳性反应物质分布在胞质，胞核呈阴性反应。

The results showed that K562 cells adhesively cultivated with MSC were inhibited and cells in G0/G1 increased (P.05), cells in S phase decreased (P.05) and those in G0/G1 increased (P.01), compared with that cultivated in suspension.

结果表明：与悬浮培养比较，黏附培养K562细胞增殖受抑，G0/G1期细胞比例增加(P.05)，S期细胞比例下降(P.05)，G2/M期细胞比例增加(P.01)。

We hypothesized that endothelial cells may play an important role in cholesterol efflux from cells by transformating it to oxysterols, which can prevent the lipid aggradation into the tissue under endothelial cells.

如果血管内皮细胞可将过多的胆固醇等脂类物质由细胞内排出，可阻止过多的脂质进一步向内皮下沉积，进而阻止或减缓动脉粥样硬化的发生和发展。

The key to using aberrant energy metabolism as a way to enhance site affinity that specifically targets the cancerous tumor cells' microenvironment that produces measurable tumor selective alkalinization involves administering an alkaline composition that has little toxicity to normal healthy viable cells which preferentially targets and elevates the pH level of the tumor micro-environment, thus causing the non-viability and apoptosis of tumor cells.

可使用异常能量新城代谢来提高癌瘤位置的亲和力，使癌瘤进行具有可测性、可选性的碱化，从而专门改变癌瘤细胞的微环境。其关键在于使用一种对正常健康活性细胞无毒性的碱性制剂，能优先提高癌瘤微环境的pH水平，从而使癌瘤细胞不具有生存能力，促使其凋亡。

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

The mechanism of PPS accelerating IEC-6 cells migration was also researched with gene-chip technique. The Altas Clontech Rat cDNA Chip which consisted of 1176 spots representing different functional categories such as heat shock/stress, cell cycle, transcription factors, hormones, metabolism, etc was used. Result showed in cell migration model of IEC-6 cell line, compared with control group, expression of 38 genes in total 1176 genes varied in cells treated by PPS, including 21 genes up-regulated and 17 genes down-regulated. Date analyze showed the mechanism of PPS accelerating IEC-6 cells migration mainly related with promoting the numbers and activity of cellular ion channel, especially 〓 channel.

从以上实验结果中我们可以得出以下结论：四君子汤总多糖具有促进IEC-6细胞增殖、迁移、粘附的作用；四君子汤总多糖无论是对IEC-6细胞增殖、或者迁移的作用均优于各单味药多糖，可能是各种单味药多糖综合作用的结果；多糖可能是四君子汤"益气健脾"药效的主要成分；小肠上皮细胞可能是四君子汤总多糖的作用靶点之一，提示临床采用四君子汤及"益气健脾"法治疗"脾虚证"的疗效机制可能与增强机体的整体免疫和肠道粘膜免疫功能有关。

Expression of Sox2 in the chick retina was detected in progenitor cells, in cells at a discrete location in the layers of amacrine and ganglion cells, and in Muller glia.

在多能干细胞中检测到了鸡视网膜中Sox2的表达，其位于无长突细胞，节细胞和Muller胶质细胞层的离散位置的细胞中。

Here we show that VacA permeabilizes the apical membrane of gastric parietal cells and induces hypochlorhydria.The functional consequences of VacA infection on parietal cell physiology were studied using freshly isolated rabbit gastric glands and cultured parietal cells.Secretory activity of parietal cells was judged by aminopyrine uptake assay and confocal microscopic examination.

为此，我们建立了原代培养的胃腺、胃壁细胞等研究模型和良好的评估壁细胞分泌能力的技术平台，包括氨基比林吸收实验、激光共聚焦扫描技术等，对VacA感染所导致的壁细胞生理特征的改变进行了深入细致的研究。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。