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Results showed endothelialcells inoculated by shimen virulent strain of CSFV have no obvious change after 12h,24h;At36h,cells become be elongate and appearanced defluvium;At 48h,a few of cells started tobecome round and intercellular space become auxesis;At 72h, intercellular space becomemore auxesis, defluvium of cells was acute.

结果表明,血管内皮细胞于接种 CSFV 石门株后 12h,24h 无明显变化,至 36h 时,细胞开始拉长,个别细胞开始脱落;至 48h 时,部分细胞变圆,脱落,细胞间隙增大;至 72h,细胞拉长呈长梭状,细胞间隙很大,细胞脱落严重。3 个对照组的细胞均未出现明显变化。

Methods the rabbit arterial smooth muscle cells were cultured ex vivo, cells of 4-7 generation were divided into groups interfered with ang-(1-7), positive control groupsinterfered with ang-ii(10-6mol/l) and blank control group .cell counting with trypanblau dying and mtt were used to evaluate the growth of cells in different groups and results were compared .

体外培养兔肺动脉平滑肌细胞,鉴定后传代培养,取4~7代细胞,应用不同浓度ang-(1-7)干预,并与血管紧张素-ii(ang-ii,浓度10-6mol/l)、空白组对照。通过台盼蓝染色法细胞计数、四甲基偶氮唑蓝比色法,确定肺动脉平滑肌细胞的生长趋势变化。

This study was to compare the protein expression profiles between radiation-induced cancer cells and normal cells. METHODS: Immortalized human bronchia endothelial cell line BEAS-2B was irradiated by γ-ray to prepare malignant BR22P50 cells.

本研究通过建立辐射致癌的细胞模型,应用蛋白质组学技术观察辐射癌变细胞和正常细胞间的差异蛋白表达谱,以期获得辐射致癌相关蛋白。

In comparison,the rhizobia in mature infected cells were larger,greater in number,varied in shape,and distributed throughout the nodule cells.The rhizobia in the mature cells also had poly-hydroxy butanoic acid and curapos.

成熟侵染细胞中,根瘤菌个体较大,数量较多,多呈棒状,少数为球形或椭球形,有很多根瘤菌还呈现明显的"T"形、"Y"形或"V"形,菌体占满了整个细胞,这时的根瘤菌大多数含有聚羟基丁酸和聚磷酸盐颗粒。

Before xylem mother cells reactivated completely , the dividing fusiform cells in cambium and new phloem cells ha .

在木质部母细胞完全恢复活动之前,形成层纺锤状原始细胞的分裂和韧皮部细胞的分化已经开始。

Before xylem mother cells reactivated completely, the dividing fusiform cells in cambium and new phloem cells had appeared at the same time.

在木质部母细胞完全恢复活动之前,形成层纺锤状原始细胞的分裂和韧皮部细胞的分化已经开始。

Serum free medium containing 0,16,32,64,128/μg/mL was added 24 hours after culture and cultured for another 24 hours. Cells were collected centrifugally and resuspended in 75% alcohol. Finally, cells were fixed 4℃overnight. Then cells were stained with PI for 30 min under 4℃.. FCM was run with parameter FL2 - A.

流式细胞仪检测细胞凋亡率对数生长期的BIU-87细胞接种于培养瓶内,24 h后更换含有终浓度0、16、32、64、12μg/mL的无血清培养液,处理24 h,离心收集,75%冷乙醇悬浮后4℃固定过夜,PI染液4℃避光染色30min,流式细胞仪于FL2-A参数下检测。

The centriole lies outside the nucleus of animal cells and many fungal and protoctist cells, but is absent in cells of most higher plants.

中心粒存在于动物细胞和许多真菌及原生生物的细胞核外,但在大多数高等植物中缺失。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

Results indicated that the variation was significant from treatment to treatment. S-deficiency caused bulging chloroplasts, loosening and dishevelled grana lamella, reducing organelle, and damaged chondriosome in mesophyll cells of the leaves, and empty cells of the roots, while S-excess, however, led to densification, condensation and randomization of grana lamella of the chloroplasts of the leaves, and separation of cytoplasms from walls of the cells of the roots.

结果表明,受S素营养胁迫的水稻,其根系及叶片的超微结构均发生了显著的变化:低S胁迫会造成水稻叶片叶肉细胞内的叶绿体结构肿胀,基粒片层松驰、散乱,细胞器减少,线粒体结构被破坏,根系细胞内几乎没有内含物;S浓度高则会使叶片叶绿体基粒片层致密、浓缩,无规则化,根系细胞发生质壁分离。

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Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的:探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等,并与健康人进行比较,从而量化精神分裂症病人的认知功能损害,并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素,还对R152a水合物的放冷进行了实验研究。

Water is the construction and development of socio-economic basis,and strategic resources.

水是社会经济建设与发展的的基础性,战略性资源。