查询词典 insert cells

Objective To compare serum-free medium AIMV with standard serum-containing medium in culturing IL-2-activated natural killer cells in vitro.Methods Proliferation,cytotoxicity in vitro and expression of CD11 c of the cells cultured in the two different media were compared.To observe the tumor target cells killed by adˉherent natural killer cellsthrough electroscope.

目的 研究方面比较无血清培养基AIMV与完全培养基对体外细胞因子激活的NK细胞的支持作用方法分别用AIMV及完全培养基培养粘附NK细胞和非粘附NK细胞，比较细胞的增殖能力、杀伤肿瘤细胞的能力、表达粘附分子CD11 c 的能力，并通过电镜对A-NK细胞所杀伤的肿瘤细胞进行形态学观察。

Methods and Results— With the use of a standard embryoid body–based differentiation protocol for ES cells, iPS cells as well as ES cells were differentiated for 24 days.

方法与结果：使用标准的拟胚体结构方案分化胚胎干细胞与ips细胞，分化24天。

The SGC-7901GA cells and control cells SGC-7901Neo were cultured in vitro,and then were respectively injected into the endermic tissue of 6-weeks-old BALB/C node mice to create the animal models of tumor. The neoplasia and growth of tumor were observed. Use MTT method to detect the difference of growth capability of two kinds of cells in vitro.

先把SGC-7901~细胞和空白对照组细胞SGC-7901~进行体外培养，再把两种胃癌细胞接种于6周龄BALB／C裸鼠皮下，建立胃癌的裸鼠动物模型，观察裸鼠成瘤情况。

The scientists noted that specialized cells generated from the AFS cells included all three classes of cells found in the deeloping embryo - termed ectoderm, mesoderm, and endoderm.

科学家还发现从羊水衍生的干细胞中生长出来的特定的细胞包含了发育的胚胎的三种细胞类型——外胚层、中胚层和内胚层。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

ESCs are traditionally regarded as ICM cells, though some scholars believe they are more closely resemble cells from the epiblast. But recent evidence about cell molecular markers indicate that ES cell's characteristics resemble early germ cells.

传统观念常常把ESCs等同于ICM细胞，也有学者认为ESCs更象上胚层细胞，而在已知的分子标记基因方面，ESCs所具有的特征更接近体内早期生殖细胞。

The reason is the tumor cells escape the immunosurveillance whose mechanism is very complex. In general, the explanation is based on two aspects, one is that the tumor cells can passively escape the host immunosurveillance when the host immnue state is low and another is tumor cells can actively secrete factors to down-regulate immune function.

肿瘤逃避免疫监视的机制非常复杂，但总体上可从宿主免疫状态及肿瘤细胞等方面来解释，在宿主免疫状态低下时，肿瘤细胞可以被动地逃避免疫监视，但是肿瘤细胞也能主动地释放一些活性物质，下调免疫杀瘤作用，该作用主要通过两个途径，即对免疫细胞功能的调节和免疫细胞数量的调节。

Neural cell adhesion molecule L1, discovered in the 80s, is one of the matters that can promote nerve regeneration. It can mediate the interaction of cell-to-cell and cell-to-matrix, take great efforts on neurite outgrowth and fasciculation, migration of neural cells, formation of the myelin, structure of neural trace, transmembrane signal transduction, inhibiting apoptosis of neural cells, even on immune system and formation of the tumor. Li is discovered on the mouse's Cerebellar membrane primarily. Thereafter, it also is found in post- mitotic neurons, pre- and non-myelinating Schwann cells and so on.

神经细胞粘附分子L1（L1）是80年代发现的具有促进神经再生作用的物质，它能介导神经细胞与神经细胞之间的粘附，在神经轴突的生长、聚集，神经细胞的转移，纤维髓鞘的形成，神经通路的构建及信号的转导，抑制神经细胞的凋亡甚至在免疫系统、肿瘤的发生等方面均具有非常重要的作用。L1最初是从小鼠的小脑膜中发现，后来发现在有丝分裂后的神经元，髓鞘形成前及非形成髓鞘的雪旺细胞等组织中同样存在。

Recent researches have shown that FOXP3(forkhead transcription facter3),which is a critical transcription factor in regulatory T cells development,plays a very important role in regulatory T cells development,This article gives an overview about molecule structure function,the relation of CD+4CD+25 Treg cells in development and function,and the meaning of clinical measure.

最近研究发现，叉头状转录因子3（FOXP3）对于调节性T细胞的发育和功能具有重要作用，是调节性T细胞发育的一个关键转录因子。文章就FOXP3的分子结构功能、与CD+4CD+25Treg细胞在发育和功能上的关系及临床检测的意义予以综述。

In young human umbilical vein endothelial cells, human aortic endothelial cells, and human coronary artery endothelial cells, miR-217 induces a premature senescence-like phenotype and leads to an impairment in angiogenesis via inhibition of SirT1 and modulation of FoxO1 (forkhead box O1) and endothelial nitric oxide synthase acetylation.

在婴儿脐静脉内皮细胞、人类主动脉内皮细胞以及冠状动脉内皮细胞中，miR-217可以诱导这些细胞出现一种早衰样表型，进而通过抑制SirT1的表达、调控Fox01以及内皮细胞一氧化氮合酶的乙酰化来损伤新生血管的形成。

He has to relate with three women in the movie, and yet remain implicitly faithful to the unseen Lucinda.

让克劳德布里亚里成功地饰演试验了一个难度很大的角色：和三个女人打交道，但还要底下对观众未曾谋面的卢馨达保持忠诚。

The experiment was conducted to study effects of inulin on the growth performance, indices of immune organs and serum immunoglobulin of laying chicklings as well as the performance of inulin which replaced the antibiotics in diets by adding 0.2%, 0.4% and 0.8% of inulin, respectively.

试验旨在通过在蛋用雏鸡日粮中添加0.2%、0.4%和0.8%的菊粉，研究菊粉对蛋雏鸡生长性能、免疫器官指数和血清免疫球蛋白的影响，以及菊粉替代抗生素对蛋雏鸡的应用效果。