查询词典 fermentation

32 U/mL enzyme activity can be detected in medium after fermentation. By optimizing the zymotic medium and fermentation conditions, recombination B. subtilis can express as high as 110.02 U/mL of Alkalineβ-mannanase. Cycle of fermentation needs 5 days(4 days is needed in induced expression ), then enzyme activity maintained at the highest level, less than 5 % enzyme activity was lost in next 2 days.

为了继续对碱性β-甘露聚糖酶进行高表达研究，试验采用了芽孢杆菌发酵培养基来进行产酶研究，初步诱导酶活达到29.32 U/mL，通过对发酵条件的优化，该重组枯草芽孢杆菌的产酶水平有了大幅度的提高，最高可达110.02 U/mL。

The fermentation condition of Acetobacter xylinuml.1812 has been optimized by single factor and orthogonal experiments including the components of medium and the initial fermentation condition and the fermentation parameters.

通过单因素和正交试验，优化了木醋杆菌产细菌纤维素的发酵条件，包括培养基成份的优化、初始发酵条件的优化和发酵动力学参数的测定。

The recoveries for 2,3-BD and acetoin were 91%and 92%,respectively.Aqueous two-phase extraction of 2,3-BD from real fermentation broth,the removal ratio of cells was above 99%,and the soluble proteins were about 85%in the top phase,which resulted in easy recovery of 2,3-BD from the top phase by vaporization because a viscous slurry was not formed by glucose,cells and soluble proteins at high temperature as in traditional separation methods.In addition,the cells recovered from the fermentation broth could be used as inoculum for further fermentation.

结果表明，目标产物2，3-丁二醇和乙偶姻主要富集在上相中，而葡萄糖主要富集在下相中；当异丙醇和硫酸铵的质量浓度分别是34%和20%时，2，3-丁二醇和乙偶姻的分配系数分别为8.29和8.98，回收率分别达到91%和92%；采用该条件对发酵液直接进行萃取，细胞的去除率在99%以上，可溶性蛋白去除率可达85%。

The optimum initial substrate concentration for cultivation of inoculum was 8%.The optimum conditions for batch fermentation were 0.04vvm of air flow,5.8 of pH value.The fed-batch fermentation showed that the productivity of Klebsiella pneumoniae CICC 10011 was better than K.pneumoniae CGMCC 1206.The maximum concentration of 2,3-butanediol and acetoin was 103.38 g/L (90.98 g/L of 2,3-butanediol),and the yield reached 82.2%of its theoretical value.The fermentation conditions for K.pneumoniae.

确定种子培养的最适初始底物浓度为8%；间歇发酵的条件为：微氧发酵，通气量为0.04vvm，pH值为5.8；通过批式流加发酵实验表明：Klebsiella pneumoniae CICC 10011的代谢产2，3-丁二醇能力要远高于K.pneumoniaeCGMCC 1206，2，3-丁二醇最高浓度达90.98g/L，2，3-丁二醇与乙偶姻浓度之和最高达103.38g/L，转化率为理论最大值的82.2%，其发酵工艺还有待于进一步优化。

Results showed that all the three sweet sorghum varieties contained plentiful sugar, which can provide enough carbon source for alcohol fermentation, however, total nitrogen and some mineral elements（Fe3＋）cannot meet the needs of alcohol fermentation. Activity of most microbes in stalk juice can be inhibited and the sugar can be maintained when the stalk juice was condensed to 4～5 times. Due to the reasons that the activity of acetous microbe or hydrolysis enzyme inside stalk juice can be maintained, ratio of reducing sugar to total sugar was increased with storage time at 55～60℃,0.15 MPa vacuum condensed condition, which can be beneficial for the purpose of alcohol fermentation.

结果表明，三个品种都含有丰富的糖分，可以为酵母菌发酵制取酒精提供良好的碳源，但总氮和某些矿物质元素（如：Fe3＋）不能满足酒精酵母的营养需要；甜高粱茎秆汁浓缩至4～5倍时，可以抑制汁液中大多数微生物的活动，使其中糖分不受损失；在试验设定的真空浓缩工艺条件下（55～60℃，0.15 MPa）甜高粱茎秆汁中产酸微生物或水解酶类仍保持活性，使得还原糖占总糖的比率随贮藏时间延长而升高，这有利于酒精发酵。

Finally, the effect of the complete recycling use of distilled effluent on ethanol fermentation was examined in order to save the cost for the treatment of waste distillage and promote cleansing production. Based on the data of 13 circulations, although the average fermentation time needed to be extended to 30~40 hours, over 12.5% ethanol was achieved, at the same time, residual reducing sugar and total sugar were maintained below 0.70% and 0.90%, which indicates that the negative effect of the completely recycling use of waste distillage was limited, and the waste distillage can be completely recycled within the fermentation system.

在此基础上，从减轻废糟液处理负荷，实现清洁生产的需要出发，进行了无种子罐条件下酒精蒸馏废液直接全循环使用的自絮凝颗粒酵母酒精连续发酵实验研究，十三次全循环运行得到的实验数据，与无废液循环的实验结果相比，虽然平均发酵时间略增至30h～40h，但发酵终点酒精浓度仍达到12.5％以上，残还原糖和残总糖分别在0.70％和0.90％以下，说明循环积累物对发酵的影响有限，废糟液直接全循环使用的工艺技术路线可行。

By comparing with the curves of classical antibiotics in Doskochilova system, the antibiotic type of fermentation broths was identified. The antimicrobial tests showed, the inhibition rate of alt the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times. It showed strongest inhibition effect against Erwinia carotorora (17.75 mm). Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%, respectively.

发酵液的抑菌活性测定结果表明，放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用，其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上；供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强，抑菌圈直径为17.75mn；放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100和71.39%。

The fungicidal activity of methanol extract was higher than that of ethylacetate extract. The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Botrytis cinerea, Alternaria longipes, Glomerella cingulata, Curvulavia lunata, Gibberella zeae, Valsa malt under the concentration of 4000μg/mL, the inhibiting ratio against pathogen growth was ranging from 62.9%~85.1%; The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, the EC50 values was 62.5328μg/mL; The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Bacillus cereus , Bacillus subtilis.

其中甲醇提取物的抑菌活性较乙酸乙酯好，在4000μg/mL浓度下对番茄灰霉病菌、烟草赤星病菌、苹果炭疽病菌、玉米弯孢叫斑病菌、小麦赤霉病菌、苹果腐烂病菌6种植物病原真菌均有抑制作用，抑制率在62.9%~85.1%；抑制孢子萌发试验表明：菌丝体甲醇提取物对烟草赤星病菌的毒力较好，其EC50仅为62.5328μg/mL；抑制细菌活性表明菌丝体甲醇提取物对蜡状芽孢杆菌和枯草芽孢杆菌有一定的抑制作用，而对大肠杆菌无明显的抑制作用。

And Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp.

该菌株的发酵液对大豆胞囊线虫二龄幼虫具有较高的毒杀活性并显著抑制胞囊孵化，不同稀释浓度发酵液的处理和无菌水处理有显著差异，发酵原液的杀线虫效果可达到90％以上，温室防效试验效果明显。同时该菌株对根结线虫Meloidogyne spp。

The process and condition in the preparation of dextran through the fermentation of Leuconostoc mesenteriodes 0326 were discussed in the paper.The adhesive viscosity,pH value, the production of fructose and dextran,variation of molecular weight and the fermentation inducer were investigated,and the related curves of the dextran fermentation presented.

文章对由肠膜状明串珠菌L.M0326发酵生产右旋糖酐的工艺过程及条件进行了探讨，通过对其发酵过程中的粘度、pH值、果糖、右旋糖酐生成和分子量变化的研究及对发酵诱导物的考察，得到了右旋糖酐发酵工艺的相关工程曲线。

Tom: You are right, he worked hard all the time.

你是对的。他一直都是努力工作。

Take shortcomings,failures,and mockings of others as commonplace and a drive to complete oneself,hold your own opinion over the evalution and disscution divested from others,afterwards sink into the state of eliminate mistakes if any or encourge oneself being not being influenced by the sayinngs of others or having no ideas to do.

把缺点、失败及别人的耻笑等看成是一种常事，当成完善自己的动力，对别人的评价和议论自己心中有主见，做到&有则改之，无则加勉&，不为人言所左右或无所适从。