查询词典 clone

Though commanded by Commander Cody, the 212th Attack Battalion was also led by High Jedi General Obi-Wan Kenobi, who developed a friendship with the battalion commander during the Clone Wars.

历史 虽然是由指挥官柯迪领导，第212攻击营也由特级绝地上将欧比王肯诺比指挥，复制人战争期间与指挥官柯迪发展了友谊。

Keywords : pomp18 gene;clone;expression;Chlamydophila abortus

中文关键词： pomp18基因；克隆；表达；流产嗜性衣原体

Objective To clone the BP26 gene of B. abortus, highly express in K. coli and purify the expressed product. Methods Extract the genomic DNA of B.

目的 克隆布鲁氏菌BP26基因，并在大肠杆菌中高效表达及纯化BP26蛋白。

Swine;Chlamydophila abortus; omp1 gene;clone; prokaryotic expression

猪；流产嗜性衣原体；omp1基因；克隆；原核表达

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

The acidophile s composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis.

采集了酸性矿山废水区域酸性沉积物样品，运用16S rDNA克隆文库技术和变性梯度凝胶电泳技术，研究了沉积物中的嗜酸菌类群以及它们的群落结构。

These are not aftermarket or clone bridges - they're the real thing.

这些都不是售后或克隆桥梁-他们是真实的东西。

MSCs were cultured in soft agar for 2 weeks to observe the clone growth.

结果 MSC在相应的诱导条件下可以向骨、脂肪和神经细胞分化。

Years later, the insane Jedi clone Joruus C'baoth boasted proudly that he killed the Guardian and took his place, however the ravings of a mad clone are not to be taken at face value.

多年以后，疯狂的黑暗绝地克隆人乔洛斯·克暴斯吹嘘说他干掉了守卫并取而代之，但根本没人会把一个疯子克隆人的胡言乱语当回事。

The first stormtroopers were clone troopers that survived the Clone Wars.

第一批冲锋队员是克隆人战争中幸存下来的克隆人士兵。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。