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Abstract］ Objective To explore the effect of radiotherapy on the auxesis of Hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.Methods After radiotherapy with different doses,the Hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.

目的 探讨放射对宫颈癌Hela细胞系再增殖的影响，宫颈癌是否有必要实行非常规分割放疗提供定的理论基础方法在不同剂量的放射作用下，以台盼蓝染色细胞计法分析Hela在照射后不同时间点的潜在倍增时间的变化；以克隆形成实验检测其同期克隆源性细胞存活比率，并将两者结合分析。

Abstract］ objective to explore the effect of radiotherapy on the auxesis of hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.methods after radiotherapy with different doses,the hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.results （1）tpot shortening was observed in hela cell after radiotherapy.

目的 探讨放射对宫颈癌hela细胞系再增殖的影响，为宫颈癌是否有必要实行非常规分割放疗提供一定的理论基础。方法在不同剂量的放射作用下，以台盼蓝染色细胞计数法分析hela在照射后不同时间点的潜在倍增时间的变化；以克隆形成实验检测其同期克隆源性细胞存活比率，并将两者结合分析。

The results indicated that CIRP in the organisms could be induced at low temperature and might protect the organisms from the cold damage. The amino acid sequences deduced via cDNA clone were 100%, 99.4%, 95.5%, 67.4%, 58.4%,76.9%, and 79.1% identical to those of the CIRP in mice, rats, human, bullfrog and axolotl cells, and Xenopus embryos and oocytes, respectively.

根据克隆的cDNA所推测的氨基酸序列与GenBank上公布的小鼠、大鼠、人类、牛蛙、美西螈、非洲爪蟾胚胎细胞和卵母细胞的CIRP氨基酸序列同源性分别为100%、99.4%、95.5%、67.4%、58.4%、76.9%和79.1%。

One cDNA clone was identified by immunoscreening from a cDNA expression library prepared from Babesia gibsoni merozoite mRNA and its complete nucleotide sequence was 2108bp in length,including one single open reading frame and encoding a 62ku protein which had high homology with that of apical membrane antigen-1(AMA-1) of other species.

通过免疫筛选法，从构建的犬吉布森巴贝虫cDNA基因表达文库中筛选到1个cDNA克隆，此cDNA核苷酸全长2108bp，包含1个完整的开放阅读框，编码蛋白的分子质量约为62ku。

The objective of this research is to clone the expression element of a major secretory protein gene from Bacillus sp.

本研究的主要目的是克隆芽孢杆菌胞外高丰度蛋白的表达元件，为今后构建表达载体奠定基础。

Objective To clone and identify a protease gene from a novel Bacillus subtilis.

目的 从新分离的枯草芽孢杆菌中克隆和鉴定蛋白酶基因。

You can also clone your DVDs so that you have a backup copy or to distribute copies to others.

您也可以刻录自己的DVD，这样你有备份，并且可以拷贝给其他人。

Bacara took it with zeal; Ki-Adi-Mundi recorded on several occasions that he found Bacara's aggressive tendencies to be remarkable for a clone.

而奇－亚地－蒙地在好几次的场合中，发现到巴卡拉积极想成为卓越复制人的意图。

This caused the insect kingdom to clone a new variety of spider known as the Black Widow.

这导致昆虫王国模仿复制了一个蜘蛛的新品种——黑寡妇。

Bly was one of the first generation of clone commanders to be trained by the ARC trooper Alpha.

布莱是ARC士兵阿尔法训练的第一代克隆人指挥官之一。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。