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It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

According to the partial correlation coefficient between the growth of forest and leaf nutrient,the N、 K content in leaf of clone S2 affected the growth of breast-height diameter mostly,and influenced the growth of tree height were Mn 、 P 、 N、 Cu content.The K、 N、 P content in leaf of clone S8 affected the growth of breast-height diameter mostly,and influenced the growth of tree height were Ca、 N content.

4从林木生长与叶片营养的偏相关系数大小来看，影响无性系S2人工林胸径生长的主要是叶片中N、K元素含量，而影响树高生长的主要是叶片中Mn、P、N、Cu元素含量；在无性系S8人工林中，影响胸径生长的主要是叶片中K、N、P元素含量，影响树高生长的主要是叶片中Ca、N元素含量。

But on the other, the therapy clone is different with procreative clone in essence.

在严格监管下进行的治疗性克隆研究，不仅不会损害人类尊严，相反，对挽救人类生命和增进人类健康有着广阔前景和巨大潜力。

The results showed that two reduplicate clones (clone 24 and clone 34) were found and the efficiency of different is 94.4%.

用DNAssist序列分析软件对测序结果分析比较，cDNA序列在GenBank数据库中进行同源性比较。

The primary result reveals that the normal expression of Clone C103 is profoundly regulated by endogenous auxin in maize root tip, and the expression of Clone C103 maybe has some relationship with statolith in the periphery of central cap.

由此表明玉米根尖内源生长素影响根冠特定基因Clone C103的正常表达；Clone C103在根冠的表达位置推测该基因正常表达可能与中心根冠外周的平衡石有关。

There are predominant clone groups among Vibrio cholera strains of different periods and districts, as well as different clone groups.

不同年代和地区之间流行的霍乱弧菌存在一定的优势克隆群，但也存在不同的克隆系。

Sheldon : I'm sorry, but I'm not going to watch The Clone Wars TV series until I've seen The Clone Wars movie.

对不起，我现在不看克隆人战争的电视剧，我得先看电影版。

I'm sorry, but I'm not going to watch the Clone Wars TV series until I've seen the Clone Wars movie.

抱歉，可在看电影版《克隆战争》之前，我是不会去看电视剧版《克隆战争》的。

This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes. An Amorpha fruticosa cDNA clone encoding UDP-glucose pyrophosphorylase, a key enzyme producing UDP-glucose in the synthesis of sucrose and cellulose, was cloned by using this method.

本论文利用此方法首次从紫穗槐中克隆了与纤维素合成前体----尿苷二磷酸葡萄糖的代谢有关的尿苷二磷酸葡萄糖焦磷酸化酶基因和与木质素合成相关的4-香豆酸：CoA连接酶(4CL)基因的全长cDNA序列，并由此推测出相应的氨基酸序列。

Clone the embryonated egg and is used for measuring various hereditary diseases, the fetus of the development among embryo and uterus that clone

将受精卵克隆用于检测各种遗传疾病，克隆的胚胎与子宫中发育的胎儿遗传特征完全相同。

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

Let's see what you got.

让我看看你的东西