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In order to clone AVR-Pia, the flanking sequence of MS220K4 was used as a probe to screening the TAC library and 17 positive clones were obtained. Further restriction enzyme mapping and Southern blot analysis on 10 of the 17 positives clones narrowed the avirulence gene to a region of 10 kb.

为克隆AVR-Pia基因，以MS220K4的侧端序列为探针筛选稻瘟菌基因组TAC文库，得到17个阳性克隆，通过对其中10个进行的限制性内切酶分析并结合Southern杂交确定了目的基因所在区域。

The composition diversity of microbial community shows that 13 genuses of microorganisms were identified by the technologies of clone labrary and isalation of pure strain and the dominant genuses were Hydrogenophaga,Pseudomonas,Bacteroides and Clostridium taking up 78% in one hundred of positive clones.

复合系组成多样性的研究表明，通过克隆文库构建和单菌株分离共确定出13个菌属的微生物，优势菌群有Hydrogenophaga、Pseudomonas、Bacteroides和Clostridium，占100个阳性克隆子的78%。

Newly built at the time of the Clone Wars, the BesGas Three tibanna refinery was thought to have been destroyed when the Confederacy attacked Bespin.

在克隆战争期间新近建设的贝斯平第三提班纳气体精炼厂被认为在独立星系联盟袭击中被摧毁了。

It's been a year , and people would ask me if I was going to appear in The Clone Wars -- if Boba was coming back.

从最初的录音阶段开始到现在有一年的时间，人们会问我会不会出现在《克隆人战争》里——波巴会不会回来。

It is the first time to clone the BiVP gene from the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell. The purified recombinant protein fulfilled the needs of further studying the bioactivity of this protein.

首次在红光熊蜂中克隆到蜂毒蛋白酶基因，在昆虫杆状病毒表达系统中进行了表达，并对表达产物进行了纯化，为深入研究BiVP的生物活性奠定了基础。

Objective To clone the Bombus ignitus venom protease gene screened from the expression sequence tags of the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell.

目的 克隆表达序列标签中筛选出的红光熊蜂蜂毒蛋白酶基因，在草地夜蛾细胞Sf-9中进行表达。

For example,the height,the diameter at breastheight and the volume of B301 of 8 years were 39%,72% and 255% largerrespectively than those of the contrast of YiXian Female clone of P.tomentosa.

如此之高的生长量，甚至已接近速生黑杨的水平，具有十分可观的增产效益。

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

Osteoblast clone cultivated by adult rat calvaria as culture model in vitro, which can be used to screen new anti-osteoporosis drugs with promoting osteogenic action.

本研究从成年SD大鼠颅骨分离培养成骨细胞，方法简便、快速，是筛选促进成骨细胞骨形成的抗骨质疏松新药的良好体外模型。

One such cell, led By Soontir Fel's clone Carib Devist, defected to the Republic.

其中一个由苏提尔克隆体加里布·德维斯特领导的克隆人投奔了共和国。

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

Let's see what you got.

让我看看你的东西