查询词典 clone

Objective To clone human intestinal trefoil factor (hITF/hTFF3) gene into an eukaryotic expression vector for its expression in eukaryotic cells.

目的 构建人肠三叶因子(hITF/hTFF3)的真核载体并在真核细胞中表达。

To clone new disease gene of CMT, many myelin and myelin-related gene had been analysised by homologue searching. Quaking mouse, one of the mutation mouse with the clinic feature similar to Trembler mouse (the gene mutation mouse of PMP22) with the characterization of demylination in both the central and the peripheral nervous system, its disease gene had just been cloned accession No.

为进一步克隆新的CMT致病基因，在分析出CMT已克隆的基因均为与髓鞘有关的基因后，通过筛选有关髓鞘基因，我们发现，quaking基因突变小鼠因髓鞘形成障碍而出现震颤，其中枢和周围神经均有脱髓鞘，类似于PMP22基因突变鼠"Trembler"的临床表现。

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

Two-fisted backhand, and steely nerves led many to dub her a Chris Evert clone.

双手握拍的反手击球，钢铁般沉着冷静的意志，这些东西让许多人习惯把她称作"Chris Evert二世"。

The third part is clone of tyrosinase gene.

第三部分酪氨酸酶基因克隆。

The above results suggested that clone 2# was an ubiquitously expressed novel EST.

上述结果表明，2#克隆是一个存在于多种组织中的新的EST。

Early in the Clone Wars, General Grievous was the most responsible for the spreading of Separatist territory through unallied sectors.

复制人战争早期，分离主义疆域在中立地区的扩展，大多都是葛里维斯将军的功劳。

In addition to his fee, he would be awarded an unaltered clone of himself.

除了费用，他将获得一个不作任何改造的克隆体。

The clone programming algorithm has the superior capability in local and global search, and local minimums are refrained efficiently; cross validation has the unbiased estimator property, and therefore, the problems such as over training or insufficient training are avoided.

克隆规划算法是具有局部、全局搜索能力的优化算法，能有效避免陷入局部极值；交叉验证算法的无偏估计性抑制了训练过程中"过拟合"和"欠拟合"。

Full genome of HCV ; Clone ; Transfected cell culture system ; Recombinant vaccinia virus

HCV全基因组；克隆；转染细胞体系；重组痘病毒

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

Let's see what you got.

让我看看你的东西