查询词典 clone

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

A mutation type and a wideness type were established by clone and gene sequence detection.

基因测序技术对无缺失片段进行克隆、测序，筛选出突变型及野生型。

You'd have to greatly multiply the workweek and clone the teachers.

要想真的这么做，你就得延长工作时间或增加教师的数量。

Snake:蛇: Snake 蛇 is a clone of popular Snake or Worm game.

克隆是一个流行的蛇或蠕虫的比赛。

So what scientists are going to do is that they try to clone a person, attempting to get stem cells from their "zygote". It is this action that shocks the world.

那么，科学家是做的是，他们试图克隆人，企图从"受精卵"中获得干细胞，正是这一行动引起了世界的轩然大波。

While the good qualities of ABNET are reserved,such as structure generated automatically, unsupervised and competitive learning based on clone selection and mutation,the way of initiating ABNET, expressing a antibody, updating structure is improved properly.

在保留抗体网络的结构自动生成，基于克隆选择、变异机制的无监督竞争学习等优点的同时，对抗体网络的初始化、抗体的表示方式、网络结构的更新等方面作了适当的改进。

In the centuries before the Clone Wars , the Corellian Sector was guarded by warships bigger than Acclamator -class assault ships .

克隆人战争前100年，科瑞利安星区里就巡逻着一些比拥护者级攻击巡洋舰还要巨大的战舰。

Another Amorpha fruticosa cDNA clone encoding 4-coumarate:CoA ligase (4CL), a key enzyme of phenylpropanoid metabolism related to lignin forming, was cloned ,too.A full-length UGPase cDNA was obtained.

所获得的紫穗槐尿苷二磷酸葡萄糖焦磷酸化酶基因UGPA长1832bp，其中开放阅读框1416bp，编码471个氨基酸，与其它植物的UGPase基因的序列相比有较高的同源性。

In the present study, differential display polymerase chain reaction was used to isolate three antennal -specific mRNA from the moth of cotton bollworm. Sequence analysis indicated that A4-1 clone was homologous to the glutathione S-transferase family of proteins.

利用差异显示PCR的方法分离得到了3条棉铃虫触角专一性的mRNA片段，在GenBank中进行同源搜索表明，克隆A4-1与昆虫谷胱甘肽-S-转移酶基因家族具有很高的同源性。

Besides, the genetic differentiation also existed among subpopulations. We deduced that the limited gene flow and breeding type were important factors leading to genetic differentiation. The genetic structure of Aristida pennata populations reflected its characteristics of clone reproduction and the good adaption to the severe environments.

本研究认为羽毛三芒草种群遗传多样性很丰富，遗传结构较为复杂，亚种群间遗传多样性大，亚种群间存在一定遗传分化，基因流和繁育方式是导致种群遗传分化的主要因素。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。