重组

The invention also relates to application of the recombinant virus and recombinant PPV VP2 nucleocapsids expressing the foreign genes of the recombinant virus in the aspects of bacterin immunity and so on.

本发明还涉及该重组病毒及其表达外源基因的重组PPV VP2病毒样粒子在疫苗免疫等方面的应用。

With the development of molecular biology, an efficient bacteriophage λ based Escherichia coli homologous recombination system has been developed for genetic engineering.

伴随着分子生物学的发展，一种基于λ噬菌体Red重组酶的同源重组系统已应用于大肠杆菌基因工程研究。

When the amount to be received in the future is equal to or greater than the book balance of the debt to be restructured upon modification of other terms, accounting treatment is not required at the time of debt restructuring, but the transaction should be recorded in the memorandum of that account.

如果修改其他债务条件后，未来应收金额等于或大于重组前应收债权账面金额的，则在债务重组时不作账务处理，但应当在备查簿中进行登记。

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

Cause weighty series of amount due genuinely bring into play such energetic action and effect by means of the acquirement to these problems, Can ease the business shoulders ,It is tired to assist the state-owned enterprise to come off ,Accelerate the entire national economy development .

通过对这些问题的探讨使债务重组真正地发挥其积极的作用和效果，能够减轻企业负担，帮助国有企业脱困，促进整个国民经济的发展。关键词：债务重组，会计处理方式，建议

Our laboratory also found a gene like Drosophila intersex in the testis of Cyprinus carpio. According to the significant function played by Drosophila ix in sex-determination, we are interested in the function of this gene in carp. To study the functions of cIX further, I cloned the full-length cDNA of cIX. The recombinant protein is produced and purified to serve as the antigen to induce antibody.

本实验室同样也在鲤鱼的睪丸中发现了类似Drosophila intersex的基因，鉴於intersex在果蝇中所扮演性别决定的重要功能，对於此基因在鲤鱼中所担任的功能而感到兴趣，为了进一步探讨cIX的功能，本论文完成cIX全长cDNA，设计并建构cIX的重组DNA，再利用纯化之重组蛋白诱导抗体。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Secondly, Pre-emphasis is used to improve the high boost in WWF algorithm. In the same way, using an effective frame regrouping precept improve frame overlapping and regrouping, designing an new SH window to window function's design, selecting coif wavelet as the analysis wavelet basis to wavelet basis's selection, discarding the unvoiced frames by threshold-wavelet transform to unvoiced/voiced frames' distinguish, and solving the wiener gain by the fake cepstral subtraction to Wiener gain's getting as well.

采用进行预加重处理、设计有效的帧重组方案、自定义SH窗、选用coif小波作为分析用小波基、采用加阈值小波变换法去除清音帧，和基于伪倒谱相减法求解维纳滤波增益系数等方法，分别对模型中高频提升、帧重叠与重组、窗函数设计、小波基选取、清浊音判别及维纳滤波增益系数获取等实现细节进行研究和改进。

A set of recombinant inbred lines population including 195 lines derived from a cross of Zhenshan 97B and IRAT109 was used for correlation analysis and QTL identification for coleoptile length and drought resistance index.

对由水稻品种珍汕97B和旱稻品种IRAT109构建的重组自交系195个株系的胚芽鞘长度及抗旱系数的研究表明，水分胁迫下水稻重组自交系群体的胚芽鞘长度与抗旱系数的相关系数为0。

Aximum intensity projection,curved planar reformation and volume rendering were used conjunctively to observe morphological changes of the coronary arteries,such as atherosclerotic plaques and compression.

B-MCA及邻近冠状动脉形态学的观察：采用多平面重组、曲面重组、容积再现多种后处理技术相结合观察MCA近端及远端粥样硬化的发生率。3。

Means the parent directory, so this command means to execute "toolchain.sh," which is in the current directory.

代表父目录，所以这个命令就是执行当前目录下的"toolchain.sh"。

Yes,In fact,I'm on our city ream.

是的。事实上我是我们市队的。