重组

Methods Expression plasmid was constructed with DNA recombi nant technique.

方法采用DNA重组技术构建结核分支杆菌Ag85B基因的表达载体，双酶切和聚合酶链反应鉴定重组子，阳性重组子转化大肠杆菌，并诱导表达外源蛋白。

A comparison is made the laws on debts transfer among the Civil Law Systems and the Common Law Systems. The author presents problems of debts transfer during the process of the SOEs' systematic reformation and reorganization through nomological and doctrinal analysis. Finally, the author provides legislative and judiciary suggestions and concrete measures to standardize the reorganization, protect the rights and interests of creditors, and prevent the state-owned assets from losing.

笔者对债务转移的概念、分类和生效要件进行了详细的阐述，对大陆法系和英美法系的有关债务转移的法律规制进行了比较研究，对我国国有企业重组改制中的债务转移问题，从法理上、学理上进行了分析，指出了国有企业重组改制存在的问题，为规范国有企业重组改制的行为，保护债权人的利益，防止国有资产流失，提出了立法上和司法上以及具体操作上的具体对策。

Methods We studied 975 nulliparous women who delivered between Jan 2008 and May 2008. Women were divided into four groups according to their prepregancy BMI: underweight group (n=93); normal weight group (n=604); overweight group (n=176) and obese group (n=102), we observed the relationship between the prepregnancy BMI with pregnant outcomes.

2008年1月至5月在我院住院并进行阴道试产的初产妇975例，按照孕前体重指数分为低体重组(BMI8.5)93例、正常体重组(18.5≤BMI3)604例、超重组(23≤BMI5)176例、肥胖组(BMI≥25)102例，观察孕前BMI与妊娠结局的关系。

The new obligation reorganization standard the behavior of enterprise 's obligation reorganization , keeping the enterprise from controlling profit and prettify the accounting statement make use of this reorganization .

新的债务重组准则极大规范了企业的债务重组行为，防止了企业借重组之机操纵利润，粉饰会计报表。

AIM: To detect the stability of the recombinant adenovirus plasmid containing major outer membrane protein gene from Chlamydia psittaci in HEK293 cells, to evaluate whether cross reactivity exists or not between recombinant adenovirus and positive serum of Egg Drop Symdrome, and to evaluate whether SPF chicks vaccinated with the recombinant adenovirus were protected or not when challenged with virulent CpL strain.

目的： 检测重组腺病毒质粒在HEK293细胞中的稳定性；重组腺病毒是否与减蛋综合征阳性血清发生中和反应，以及用重组腺病毒免疫SPF小鸡是否产生保护。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Starting from the particularity of Bank,this article makes an analysis of the current situation of the national urban commercial bank,and puts forward a scheme—Rearrangement of assets,by which the national urban bank can extricate themsel...

从徽商银行的特殊性出发，对我国城市商业银行进行现状分析，提出我国城市银行走出困境的方案——资产重组。以徽商银行成功资产重组为案例，分析其过程，从而为其他城市商业银行资产重组模式的正确选择提供借鉴作用。

So in order to get recombinant NT-3, the rat NT-3cDNA was reconstructed in a mammalian expression vector and transfected into incasing CHO cells by liposome-mediated method. The clones of the cells transferred with rNT-3cDNA were selected by G418. The Chinese hamster ovary cell line stably producing recombinant rat NT-3 was established.

因此，为了获得基因重组NT-3，研究其生物效应，本文成功构建了重组大鼠NT-3基因真核生物细胞稳定表达载体，并利用脂质体介导，转染进CHO细胞；经G418筛选，建立了中国仓鼠卵巢细胞系，稳定表达重组大鼠NT-3。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。