重组

Combined with the practices of reformation and management of basic MCH organization in Guangzhou Liwan area. Study the way of reformation and management.

结合广州市荔湾区基层妇幼保健组织重组与管理的实践，对重组的做法，以及管理进行了探讨。

Shanghai light breeds course of study built 30 thousand milker raise base, signed the production that suckle a source with outskirt county farmer, collect a contract to make; 3 yuan of milk industry of Beijing are bought in succession breathe out alliance Hailaer is milkings factory, full continent Lizalai if 85 ％ of company of milkings of husband of card of Er milkings factory, Beijing are foreign equity; Shijiazhuang industry of 3 deer milk early or late with Tangshandi 2 milkings factory, Cheng Dehua breeds the 10 milkings enterprise such as company of milkings of factory of milkings of grass of source of course of study, buy, Jun Lebao undertook recombining rather; Heilongjiang is over industry of the milk that amount to hill and implementation of industry of crane king milk are amalgamative recombine, make a pine tender the 5 milkings factory of Campagna, 8 product line, treatment milkings ability increases 15 thousand T again, the development road of these successful companies makes clear: Produce advantage of ground predestined relationship, enterprise of bibcock of implantation agriculture industrialization, walk along dimensions to change management way, executive brand strategy, can make cultivate industry of course of study, aquaculture, treatment to obtain industrial chain type to develop, flourish already local economy, enhanced people health again, form the benign loop of business economic benefits and social benefit.

上海光明乳业建立了3万头乳牛的饲养基地，与郊县农户签订了奶源生产、采集合同制；北京三元乳业相继收购呼盟海拉尔乳品厂、满洲里扎莱若尔乳品厂、北京卡夫乳品公司85％外方股权；石家庄三鹿乳业先后与唐山第二乳品厂、承德华宁乳业、沽源牧场乳品厂、君乐宝乳品公司等10家乳品企业进行了重组；黑龙江完达山乳业与鹤王乳业实现合并重组，使松嫩平原的5个乳品厂，8条生产线，加工乳品能力又增加1.5万t，这些成功企业的发展道路表明：发挥地缘优势，培植农业产业化龙头企业，走规模化经营之路，实施品牌战略，可以使种植业、养殖业、加工业获得产业链条式发展，既繁荣地方经济，又增强了人民健康，形成企业经济效益和社会效益的良性循环。

Chain reconfiguration modular robots form systems with many degrees of freedom which are capable of being reconfigured to form arbitrary chain-based topologies.

链重组的模块化机器人具有非常多的自由度，能够重组成多种基于链的拓扑结构。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

Methods Using prime-boost immunization strategy,BALB/c mice were inoculated muscularly with HIV-2 gp105 recombinant DNA vaccine and fowlpox virus.

目的：探讨应用prime-boost免疫策略进行HIV-2外膜蛋白重组鸡痘病毒与重组DNA疫苗联合免疫引起小鼠的免疫应答。

Methods BALB/c mice were inoculated muscularly with HIV-2 gag recombinant DNA vaccine, or recombinant fowlpox virus, or both.

大量制备并纯化HIV-2 gag重组DNA疫苗和重组鸡痘病毒，以肌肉注射的方式免疫BALB／c小鼠，ELISA法检测小鼠血清HIV-2抗体，流式细胞仪测定CD4^＋、CD8^＋T淋巴细胞亚类数量，乳酸脱氢酶释放法检测脾CTL对HIV-2靶细胞的杀伤活性。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

The recombinant adenovirus containing noggin gene has been successfully constructed by homologous recombination in bacteria.

应用细菌内同源重组法成功构建了含小鼠noggin基因的重组腺病毒载体。

However, the problems in the reorganization of the listed companies could not be omitted.

我国上市公司的重组成绩是显著的，但是在重组中存在的问题也是不容忽视的。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。