重组

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

Recombinant proteins are created indirectly, by first recombining their DNA, and then expressing the protein from the recombinant DNA.

重组蛋白是用间接的方法得到的，首先将它们的DNA重组，然后从重组DNA表达出蛋白质。

Chapter 4: On Barriers and Countermeasures for Enterprise Assets Recomposing.

因此，克服目前存在的相关体制政策障碍，建立完善的资产重组的法律环境，有助于规范企业资产重组行为，实现预期的重组效果。

The construction of viruses were identified by enzyme digestions, PCR amplification, sequencing, light and electronic microscope analysis.

采用同源重组的方法分别构建了带BMP—2基因和报告基因LacZ的复制缺陷型重组腺病毒表达载体，通过酶切、PCR扩增、序列测定和光镜、电镜观察等方法证实重组病毒构建正确。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

"On the bankruptcy reorganization of listed companies to issue shares in a major reorganization of assets to add pricing" is a "significant asset restructuring of listed companies management"(Order No. 53 of the SFC) of the forty-second Added.

对上市公司破产重组&的问题，在重大资产重组股份定价补充&是&管理上市公司重大资产重组&（令第53号证监会）的40秒的时间。

SFC yesterday officially released the revised "major asset reorganization of listed companies management approach," the forty-second, the reorganization of listed companies on major asset restructuring the pricing mechanism and approval procedures has made special provisions.

证监会昨日正式发布了上市公司管理办法修订后的&重大资产重组&的第四十二，对上市公司重大重组的定价机制和批准程序，资产重组已作出特别的规定。

Firstly, it discusses the results by many kinds of capital reform modes. For example, desquamation schism stands out the main businesses of enterprises, merger purchase realizes polygonization manage, stock transfer changes the unreasonable structure of stock ownership in enterprises, capital displace immits fine capital to quoted company.

本文首先从不同的资产重组方式入手，讨论了各种重组方式对企业价值产生影响的结果——剥离分立突出了企业的主营业务、兼并收购使企业快速实现多角化经营、股权转让改变了公司不合理的股权结构、资产置换向上市公司注入了优良资产，总之不同的资产重组方式的合理使用，对提高企业价值都会产生积极的作用。

The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^＋ and Leu2^＋LacZ^＋ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法扩增Id1的编码序列并定向克隆入诱饵质粒pHybLex/Zeo，构建重组诱饵质粒pHybLex/Zeo-Id1，酶切鉴定后转化入酵母株EGY48/pSH18-34，检测重组诱饵质粒有无非特异激活报告基因Leu2、LacZ作用；扩增并提取成人肺组织文库质粒，将文库质粒及重组诱饵质粒转化入酵母细胞，依次筛选Leu2^＋，Leu2^＋LacZ^＋克隆；设置阴性及阳性对照，重复筛选Leu2＋LacZ＋克隆并排除假阳性克隆，获取真阳性文库质粒，进行测序和同源性比对。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。