重组

Article 12When other terms of a debt are modified, the creditor shall recognize the fair value of the credit after the modification of other terms of the debt as the book value of the restructured debt and shall handle the book balance of the debt to be restructured and the book value of the restructured debt in accordance with Article 9 of these Standards.

第十二条修改其他债务条件的，债权人应当将修改其他债务条件后的债权的公允价值作为重组后债权的账面价值，重组债权的账面余额与重组后债权的账面价值之间的差额，比照本准则第九条的规定处理。

Article 7Where other terms of a debt are modified, the debtor shall regard the post-modification fair value of the debt as the entry value of the restructured debt, and shall include the difference between the book value of the debt to be restructured and the entry value of the restructured debt in the current profits and losses.

第七条修改其他债务条件的，债务人应当将修改其他债务条件后债务的公允价值作为重组后债务的入账价值。重组债务的账面价值与重组后债务的入账价值之间的差额，计入当期损益。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

The first part of the article analysis deferent kinds of reorganization and separate debt reorganization from others.

本文第一部分首先分析了重组的概念，把债务重组与其他形式的重组区分开来。

Mathematical remixing, physical remixing, chemical remixing and biological remixing are the forms of expression in economic genomics.

经济和资产的数学重组、物理重组、化学重组和生物重组是经济基因学的表现形式。

Factors involved in the expression of IFN -αwere studied. These included multiplicity of infection, cell inoculation concertration and cell growth phase. The results show that lower mulitiplicity of infection (M01.4PFU/cell) is beneficial to IFN-α production.The optimal cell inoculation concentration was 〓 cells/flask. The high efficient replication of BmNPV-IFN-α and expression were also due to the proper inoculation time of BmNPV-IFN-α, which was at 48-60 house after cell passage.

研究结果表明，重组病毒感染复数的改变对α-干扰素的产量的影响是时限性的，在一定低感染复数时（Mo1.4PFU），α-干扰素的产量较高；对于重组病毒的复制，最适的细胞接种密度为3.6～7.0×〓细胞/瓶；在细胞指数生长前期（48～60小时）接种重组病毒，对于获得较高的重组病毒效价和α-干扰素的产量都是有利的。

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代，取第5、10、15和20代重组病毒进行以下检测：用蓝斑试验鉴定各代重组病毒纯度；用PCR方法扩增猪瘟病毒E0、E2基因，进行基因序列分析；用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。