病毒

Biology technique is based on appearance symptom on sweet potato or Ipomoea Setosa after they are infected to identify virus. But it need long period and is apt to be limited by season. Based on coat protein of virus, serology detects virus during peculiar immunolog...

生物学方法是依据病毒侵染后在甘薯或指示植物上的外观症状进行识别，但检测速度慢，易受季节限制；血清学技术是利用病毒外壳蛋白的抗原性，据特异性的抗体抗原免疫学反应检测病毒存在，但制备抗血清需时间长，对含量少和无蛋白外壳的病毒无法检测；分子生物学方法是以核酸为基础，通过检测病毒核酸来证实病毒的存在，该技术具有灵敏度高、特异性强、适应范围广等特点。

Biology technique is based on appearance symptom on sweet potato or Ipomoea Setosa after they are infected to identify virus. But it need long period and is apt to be limited by season. Based on coat protein of virus, serology detects virus during peculiar immunology reaction of antibody and antigen. But it can't detect virus without CP and preparing antibody need more time. The molecular biology method is based on nucleic acid to detect virus. This technique has high sensitivity, strong veracity, wide suitability etc.

生物学方法是依据病毒侵染后在甘薯或指示植物上的外观症状进行识别，但检测速度慢，易受季节限制；血清学技术是利用病毒外壳蛋白的抗原性，据特异性的抗体抗原免疫学反应检测病毒存在，但制备抗血清需时间长，对含量少和无蛋白外壳的病毒无法检测；分子生物学方法是以核酸为基础，通过检测病毒核酸来证实病毒的存在，该技术具有灵敏度高、特异性强、适应范围广等特点。

Virus removal efficiency is determined by the real time PCR and inactivation efficiency is determined by cell-based infectivity assay.Enveloped DNA virus removal and inactivation: Real time PCR indicated that method based on gold magnetic particles coupled with antiserum has shown great superiority on virus removal. Cell-based infectivity assay indicate pasteurism has shown superiority on virus inactivation.

对两种指示病毒分别进行巴氏灭毒和偶联抗血清金磁颗粒处理，实时荧光定量PCR检测核酸变化确定病毒的去除效率，同时进行病毒的细胞感染力实验确定病毒的灭活效率，结果显示：在对DNA有包膜病毒的去除能力上，金磁颗粒法明显优于普通的巴氏灭活法；在对DNA有包膜病毒灭活能力上，巴氏法明显优于金磁颗粒法。

A virus strain was isolated from the arthrosis of broiler chickens.The virus was shown to have spherical particles with isometric symmetry,no envelope,double deck capsids,with outer capsid of 75nm,inner one of 50nm in diameter by electron microscopy.The virus was not sensitive to aether,acid and heat.The virus could multiply on the chick embryo fibroblastcell,and could cause syncytial pathologic change with the appearance of cytoplasm inclusion bodies.The challenged chickens of one-day old by nasal,oral,intramuscular and pulvillus injection displayed obvious clinical symptom of severe arthritis and accompanied with pathological changes of malabsorption and necrosis.Meanwhile,four avian reovirus isolates CL,S1133,YH and YB were examined in the cross-neutralization test.

从临床患病鸡关节病料中分离到一株病毒，在电镜下观察到病毒粒子无囊膜，有双层衣壳，外衣壳直径约75nm，内核约50nm；分离病毒株对酸、热有较强的抵抗力，对乙醚不敏感；病毒感染鸡胚成纤维细胞上能产生以融合细胞为特征的CPE；爪垫接种1日龄雏鸡表现出明显的病毒性关节炎临床症状，并伴有吸收障碍型和坏死型的病理变化；血清中和交叉试验表明，该病毒分离株与国内的2株番鸭源呼肠孤病毒分离株和国外S1133呈不同程度的交叉反应。

Some key techniques related to the close and continuous process were investigated by the application of H9N2 avian influenza virus with Vero cells, such as the susceptibility of cell to influenza virus, virus production with cell microcarrier culture method, cell bead-to-bead transfer, virus production through bead-to-bead transfer, cell culture and virus production with serum free medium, metabolism analysis, and repetitiously intermittent bead-to-bead transfer of cell for virus production to simulate the close and continuous process.

通过使用Vero细胞增殖禽流感病毒H9N2，本文针对封闭连续工艺过程的一些关键技术开展研究，包括细胞对流感病毒敏感性分析、细胞微载体培养生产病毒工艺、细胞珠到珠转移、转移后细胞对病毒增殖敏感性验证、细胞无血清培养生产流感病毒、代谢分析、可模拟连续操作的多次间歇式珠到珠转移培养细胞生产流感病毒等方面。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

To observe the antiviral activity of Algefacient and anti-inflammatory against influenza virus, cell culture technique was used in MDCK cells to get virus inhibitory rate and virus titer by MTT assay and hemagglitination test. Influenza virus infection model was established in mice, to observe the protection effect to mice death, the inhibitory effect to pulmonary index and pulmonary virus titer.

为评价清热消炎复方制剂的抗流感病毒活性，我们以病毒唑为对照，通过在体外观察病毒致细胞病变效应、MTT细胞染色检查病毒抑制率和检测病毒血凝滴度；在体内观察其对染毒小鼠的死亡保护作用，对小鼠流感病毒性肺炎的抑制作用，以及对小鼠肺内病毒增殖的影响，从而判定其抗流感病毒作用。

The nearly full-length CP sequence of 308 aa of SCMV-NCH was compared with the counterpart of SCMV, Sorghum mosaic virus, Maize dwarf mosaic virus; Johnsongrass mosaic virus, Zea mosaic virus and Pennisetum mosaic virus, the six virus species in SCMV subgroup in Potyvirus, respectively. A high level of sequence homology (86.2%) was observed between SCMV-NCH and SCMV-A, while only 56.4% to 72.2% sequence homology was found between SCMV-NCH and the other five virus species.

与SCMV亚组SCMV、高粱花叶病毒、玉米矮花叶病毒、约翰逊草花叶病毒、玉米花叶病毒、白草花叶病毒等6种病毒代表性株系外壳蛋白氨基酸序列同源性比较结果显示，SCMV-NCH与SCMV的同源性最高(86.2%)，与其他5种病毒同源性相对较低(56.4%~72.2%)。

Methods Using the well-conserved regions of the DNA polymerase gene in human herpesviruses,we synthesized two pairs of primer,including one pair of primer designed to amplify herpes simplex virus type 1 and 2,Epstein-Barr virus and cytomegalovirus,another pair of primer to varicella-zoster virus and human herpesvirus 6 by PCR.

2 0 0 2年 1～ 12月浙江大学儿童医院在疱疹类病毒高度同源序列DNA多聚酶基因中设计两对通用引物，该引物能同时扩增单纯疱疹病毒Ⅰ型与Ⅱ型、爱泼斯坦—马尔病毒、人巨细胞病毒、水痘带状疱疹病毒和人类疱疹病毒 6型(HHV6) 6种病毒。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.The mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低pH常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低pH/巴氏消毒法；人纤维蛋白原、人凝血因子Ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。