病毒

To quickly obtain more information on a novel virus, it is then possible to syphon off'' those viral sequences that stuck to their respective counterparts on the chip and to use the material to determine part of the genomic sequence.

在快速获得病毒信息后才有可能去知道病毒的基因序列，这些序列信息可以让我知道未知病毒与已知病毒的关系，这可以提供线索让我们知道病毒的来源和可能的治疗方法。

Objective:To investigate the correlation among hepatitis B virus markers in gastric juice,serum and gastric mucosa in chronic patients with hepatitis B virus infection,the influence of HBV infection on gastric mucosa in chronic hepatitis B patients,and the possibility of infection by alimentary canal.

目的：通过研究慢性乙型肝炎病毒感染者胃液、胃粘膜及血清中乙肝病毒标志物的存在情况，分析它们之间的相互关系，以探讨乙型肝炎病毒感染对慢性乙型肝炎病毒感染者胃黏膜病变的影响及乙型肝炎病毒消化道传播的可能性。

Methods The promoter of hTERT and Cox-2 was subcloned from human genomic DNA, then ligated to shuttle vector pd306 which contains the Ela and Elb gene of adenovirus to control the expression of Ela and Elb,respectively.

将hTERT和Cox-2启动子从人类白细胞基因组中亚克隆出来，并将启动子分别插入到腺病毒穿梭载体pd306上的E1A和E1B基因前，使hTERT和Cox-2启动子分别调控腺病毒必须基因E1A和E1B的表达，再将构建后的pd306和腺病毒的骨架质粒BHGE3在Ad293细胞内进行同源重组，并用重组后的病毒感染Hela细胞检测病毒对肿瘤细胞的杀伤力。

Porcine hemoglobin polymer is made in imitation as the substtitute for blood product. Pseudorabies virus is chosen as the enveloped DNA model virus and the Porcine Parvovirus is chosen as the nonenveloped DNA model virus. Complete DNA sequences of these two kinds of virus are analyzed and specific fragments are selected as examinable targets. Establish a new method based on real time PCR combined with cell infection assay to evaluate virus clearance efficiency in blood product.A new method is established to remove the virus from blood product.

本研究以戊二醛聚合猪血红蛋白模拟血液制品，选取PRV、PPV分别作为血液制品中有包膜DNA指示病毒和无包膜DNA指示病毒，针对特定两种指示病毒PRV、PPV，进行了生物信息学分析，选取特定片段作为扩增靶点进行检测，建立了一种基于实时荧光定量PCR技术的检测方法，联合病毒的细胞感染力实验，用以评价血液制品中指示病毒的灭活去除效率。

Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料，通过9～11 日龄非免疫鸡胚进行了病毒的实验室分离和传代，并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定，结果表明，4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞，标准阳性血清可特异性的抑制其凝集性，回归试验可复制出与自然发病相同的病例，病毒传代物有明显的致鸡胚矮小化作用，透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子，将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

Like other alpha herpesvirus such as herpes simplex virus type I, varicella-zoster virus and bovine herpesvirus type I, PRV also has some typical characteristics of alpha herpesvirus, including latency and neurotropism.

与同属α—疱疹病毒亚科的人单纯疱疹病毒、水痘-带状疱疹病毒和牛疱疹病毒Ⅰ型等一样，PRV具有潜伏感染和神经嗜性等α—疱疹病毒的典型特征。

The virus families I suspect in FMS, CFIDS and all sorts of these things are the viruses that used to cause "nonparalytic polio", the coxsackie and echo viruses, entero viruses that that can attack and follow nerves, just like the parlytic polio viruses.

该病毒的家庭我在柔性制造系统，CFIDS而这些各种各样的事情怀疑是所使用造成&nonparalytic脊髓灰质炎&病毒，柯萨奇和回声病毒，肠道病毒可以攻击和后续神经，就像parlytic小儿麻痹症病毒。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白，后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾，编码RNA聚合酶，含有微小RNA病毒RNA聚合酶的八个保守基序，同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

Four viral strains were found in proventricular tissues; three of them were identified as reticuloendotheliosis virus, coronavirus and Newcastle disease virus respectively. Another unidentified virus was of spherical shape, ranging from 60nm to 80nm in diameter, and could be found only among cells.

从腺胃组织的超薄切片中可观察到四种病毒颗粒，分别为IBV、网状内皮组织增生症病毒和新城疫病毒，此外还有一种呈球形，大小在60~80nm的病毒颗粒，该病毒主要分布于细胞间隙中，尚未鉴定。

An unusual isolate from watercress mottle,in association withturnip mosaic and cucumber mosaic viruses,produced small white local necrotic lesions on sapinoculated leaves of Nicotiana tabacum,N.rustica,N.glutinosa,Datura stramonium,Solanumnigra,Brassica pekinensis,B.chinensis,B.campestris,Raphanus sativa,Nasturtium officinale,Spinacia oleracea and Amaranthus mangostanus while small brown local necrotic lesions onGomphrena globosa,Phaseolus vulgaris,Dolichos lablab...

在北京香山樱桃沟野生的十字花科豆瓣菜上发现有芜菁花叶病毒、黄瓜花叶病毒以及一种未报导过的&小白斑&病毒的复合病。小白斑病毒在20多种寄主上，包括十字花科，单独汁液接种时只能产生局部坏死病斑，不能系统侵染；单独接种而能系统感染的寄主有百日菊，只产生极轻微的深浅绿花叶，甚至有时几乎无病状。小白斑病毒能在复合病情况下系统存在于豆瓣菜中是值得注意的一种现象。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。