病毒

Strains M110 and M113 isolated from Aedes albopictus and Armigeres durhami, respectively, which were fatal to suckling mice at 4 days after intracerebrally inoculation and had cytopathologic effects on BHK21 cells, showed a positive reaction to dengue antibody and could be amplified Ly using flavivirus primers and dengue type-4 NS1 gene primers and dengue type-4 NS2a gene primers.

结果 从白纹伊蚊和达勒姆阿蚊中各分离到一株病毒，分别命名为M110和M113，这两株病毒对BHK21细胞能产生明显的细胞病变，脑内接种乳鼠4d左右导致乳鼠死亡。该病毒经血凝、血凝抑制和间接免疫荧光试验提示为黄病毒。分别用黄病毒属特异引物、登革4型病毒NS1和NS2a基因片段特异引物进行RT-PCR扩增、序列测定，证实为登革4型病毒。

To establish a method to differentiate Muscovy duck parvovirus from goose parvovirus, primers GPVF/R1 and MDPVF1/R1 were designed according to the genomic sequences of GPV and MDPV to amplify the virus culture and nucleotide of GPV, MDPV, duck plague virus, duck hepatitis virus, duck reovirus, canine parvovirus and feline panleucopenia virus.

根据番鸭细小病毒和鹅细小病毒基因组的非同源序列各设计了1对引物MDPVF1/R1和GPVF1/R1，建立了一种PCR方法，用该PCR方法分别对GPV、MDPV、鸭瘟病毒、鸭肝炎病毒、鸭呼肠孤病毒、犬细小病毒和猫泛白细胞减少症病毒的病毒培养物及其核酸进行扩增。

The study was aided by the Ailuropoda Fund and designed on account of the Chengdu zoo' s topic that the Detection of the feline distemper' s serum antibody and the foundation of its immune programme about the grow wild feline animal such as Panthera pardus L, et al. The study regarded the attenuated feline panleukopenia virus as the diagnostic antigen which was isolated from the tri-combinant vaccine of Feline rhinotracheitis virus, calicivirus and panleukopenia virus . According to the tesults of Virus Physical and Chemical Character Detection, Agar Diffusion Reaction and Virion Shape Electron Microscope Observation, it was affirmed that the virion which was isolated from the tri-combinant vaccine was feline panleukopenia virus .

本研究从猫瘟热病毒、猫鼻气管炎病毒、猫鼻结膜炎病毒三联弱毒活疫苗中分离猫瘟热病毒弱毒株，通过对分离株进行病毒理化特性检测、双向琼脂扩散试验检测和病毒粒子形态电镜观察，最终确认从三联弱毒疫苗中分离到猫瘟热病毒，并以此分离株作为诊断抗原。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量，Dot blot表明病毒DNA的合成也没有受到影响，但电子显微镜超薄切片发现在0.5ug／mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别，认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子，即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响，但对病毒粒子的组装是必须的。

The "unsampled pig herd" theory was suggested by Smith and his colleagues, who concluded that "the progenitor of the S-OIV epidemic originated in pigs", and the "long unsampled history observed for every segment" of the S-OIV genome "suggests that the reassortment of Eurasian and North American swine lineages may not have occurred recently, and it is possible that this single reassortant lineage has been cryptically circulating rather than two distinct lineages of swine flu", and that "Movement of live pigs between Eurasia and North America seems to have facilitated the mixing of diverse swine influenzas, leading to the multiple reassortment events associated with the genesis of the S-OIV strain."

&无取样猪群&论，由Smith和他的同事提出，断定&流行猪源流感病毒的祖辈起源于猪&，猪源流感病毒基因上&观察到的每一个基因片断长期无样本历史&表明，&欧亚族和北美族猪流感病毒重配可能没有在最近发生，也许这种重配病毒已经神秘传播，而不是两个不同族猪流感病毒&，而且&在欧亚大陆与北美之间的生猪运输似乎促进了不同的猪流感病毒混合，导致了和猪源流感病毒毒株基因关联的多重配事件。&

The "unsampled pig herd" theory was suggested by Smith and his colleagues who concluded that "the progenitor of the S-OIV epidemic originated in pigs" and the "long unsampled history observed for every segment" of the S-OIV genome "suggests that the reassortment of Eurasian and North American swine lineages may not have occurred recently and it is possible that this single reassortant lineage has been cryptically circulating rather than two distinct lineages of swine flu" and that "Movement of live pigs between Eurasia and North America seems to have facilitated the mixing of diverse swine influenzas leading to the multiple reassortment events associated with the genesis of the S-OIV strain."

&无取样猪群&论，由Smith和他的同事提出，断定&流行猪源流感病毒的祖辈起源于猪&，猪源流感病毒基因上&观察到的每一个基因片断长期无样本历史&表明，&欧亚族和北美族猪流感病毒重配可能没有在最近发生，也许这种重配病毒已经神秘传播，而不是两个不同族猪流感病毒&，而且&在欧亚大陆与北美之间的生猪运输好像促进了不同的猪流感病毒混合，导致了和猪源流感病毒毒株基因关联的多重配事件。&

Efficiency: Bactericidal, Virucidal (incl. HBV,HCV,HIV,SARS,H5N1,Vaccinia,virua), and fungicidal100g 中含 Isopropanol 62.80g;Aqua bidest37.20g, Perfume and colorant free

生效范围：细菌，病毒（包括乙肝病毒，丙型肝炎病毒，爱滋病毒，SARS病毒，禽流感病毒，痘苗病毒）和真菌

Compared with other potyviruses, there is the highest identity between WMV-CHN with Soybean mosaic virus, the nucleotide and amino acid identity is 78.4% and 86.1%, respectively; the second are Peanut stripw virus, Bean common mosaic necrosis virus, Bean common mosaic viurs, Blackeye cowpea mosaic viurs and Zucchini yellow mosaic virus, the homologies of nucleotide and amino acid are 68.3% and 70.9%, 67.2% and 71.2%, 67.3% and 70.3%, 67.7% and 70.9%, 62.2% and 62.9% in turn, respectively. The 5′-termini of WMV is intimately correlated with evolution of viruses.

与其他Potyvirus属成员相比，同源性最高的是大豆花叶病毒，核苷酸和氨基酸同源性分别为78.4%和86.1%，其他依次是花生条纹病毒病毒、菜豆普通花叶坏死病毒、菜豆普通花叶病毒、黑眼豇豆花叶病毒和小西葫芦黄花叶病毒，核苷酸和氨基酸同源性分别为68.3%和70.9%，67.2%和71.2%，67.3%和70.3%，67.7%和70.9%，62.2%和62.9%。

Detail Contents: Genetic disorders -- Immune deficiencies -- Breast cancer -- Colon cancer -- Melanoma -- Cystic fibrosis -- Hemophilia -- Liver disease -- Cardiovascular disease -- Muscular dystrophy -- Alzheimer's disease -- Parkinson's disease -- Huntington's disease -- Viruses: the cornerstone of gene therapy -- Viruses are living crystals -- Viral genomes may be RNA or DNA -- Viruses evolved from plasmids -- Viruses know how to infect cells -- The virus as a gene vehicle -- Viruses used in gene therapy -- Ashi DeSilva: a promising start -- Clinical trials defined -- Cells of the immune system -- Adenosine deaminase -- Preliminary research -- Clinical procedure for ADA gene therapy -- The DeSilva clinical trial -- Jesse Gelsinger: down to earth -- Ornithine transcarbamylase -- Preliminary research -- Clinical procedure for OTC gene therapy -- The Gelsinger clinical trial -- The investigation -- Concluding remarks -- Future prospects -- Safer vehicles -- Reducing immune rejection of the vector -- Improved risk assessment -- Redesigning human anatomy and physiology -- Ethics of gene therapy -- The Belmont report -- Clinical trials -- Physiological enhancement -- Cosmetic applications -- Legal issues -- Regulatory agencies -- The Gelsinger legal trial -- International regulation -- Resource center -- Eucaryote cell primer -- Recombinant DNA primer -- The human genome project -- X-linked severe combined immunodeficiency (SCID-X1)-- Alzheimer's disease -- Huntington's disease.

细节内容︰遗传疾病-免疫的缺乏-乳腺癌-结肠癌-黑瘤-囊性纤维变性-血友症-肝疾病-心血管疾病-肌营养不良-早老性痴呆病-帕金森疾病-亨廷顿疾病-病毒︰基础的基因治疗-病毒在活著水晶--病毒的基因可能是RNA或者DNA --病毒从plasmids被逐步形成--病毒知道怎样感染细胞--作为一辆基因车辆的病毒--基因治疗使用的病毒-Ashi DeSilva︰有希望开始-临床试验确定--细胞的这免疫系统-Adenosine deaminase-初步研究-临床程式给埃达基因治疗--这DeSilva临床试验-婕西Gelsinger︰到地球-Ornithine transcarbamylase-初步研究-临床程式给OTC基因治疗-- Gelsinger临床试验-调查-达成评论-前景-更安全的车辆--矢量的降低免疫的拒绝-改进风险估计-重新设计人解剖学和生理学--伦理学的基因治疗-那些贝拉蒙特报告-临床试验-生理提升-美容应用-法律问题-协调机构-- Gelsinger 合法审讯-国际管理-资源中心人物-Eucaryote信元第一-Recombinant DNA 入门--人类基因工程-- X 连结的严重的结合的免疫缺陷(SCID-X1)-早老性痴呆病--亨廷顿的疾病。

The results shown the workflow can successfully find the IRES of Enterovirus genus.

首先测试在四个已知具有IRES的病毒全基因中寻找长度为205个碱基的肠病毒71型IRES Domain IV，被搜寻的目标包含肠病毒71型、牛肠病毒、鼻病毒以及C型肝炎病毒，结果显示这样的设计能够找到肠病毒属IRES序列的位置。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。