病毒

Partial DNA-A sequence comparison with other geminiviruses showed that G7 were infected with 5 begomoviruses, these viruses were Papaya leaf curl China virus, Ageratum yellow vein china virus, Tomato leaf curl China virus, Euphorbia leaf curl virus and Tobacco leaf curl Yunnan virus.

得到部分G7的DNA一A序列，经NCBI检索和序列比较分析发现了5种菜豆金色叶病毒属病毒的部分DNA序列，分别是中国番木瓜曲叶病毒、中国胜红蓟黄脉病毒、中国番茄曲叶病毒、一品红曲叶病毒、云南烟草曲叶病毒。

As showed above, we could draw the conclusion that H3 viruses could cause outbreaks or local epidemics in Shanghai for the immune of population to the new emerging antigenic drift was still unknown though there was strong immune barrier to the previous vaccine strain, H1 viruses also may caused epidemics as the population had a weak immune protection to the previous reference strains A/New Caledonia/20/99-like and part of the recent isolates were distingwishing from them and continued to evolute on the new reference strain, and H5N1 HPAI activities has expanded its geographical range thus increasing the size of the population at risk as the continuing emergence of human cases which give the virus an opportunity to evolve towards a fully transmissible pandemic strain. We should keep eyes on the human cases of avian influenza virus an its variation for the H5 isolates in Shanghai also has the possibility to infect human directly and the infection of H5, H9 viruses had previously occurred in the population especially in those contacting with animals.

综合上述分析结果，我们认为上海地区人群虽然对原有的H3亚型参考病毒株有较高的免疫保护，但H3亚型病毒HA已出现新的抗原漂移，人群现在对新变异株的保护水平尚未知，有可能在未来引起局限性暴发甚至地方性流行；人群对H1亚型流感病毒免疫保护水平低，同时H1亚型流感病毒株部分与原推荐疫苗株抗原性差别较大，并在现推荐疫苗株基础上继续变异，需警惕发生流行的可能性；H5N1亚型高致病性禽流感地域范围扩大，人间病例不断发生，存在演变成大流行株的威胁，上海市H5亚型禽流感病毒株存在跨宿主屏障感染人类的可能性，而且已发现人群尤其禽、畜类动物接触人群中存在H5、H9亚型流感病毒的既往感染，应警惕禽流感病毒的变异和人间病例的发生。

Virus hasn't cell construction. A lot of simple virus is only made of nucleus acid and protein tegument, so we can regard it as nucleoprotein. A type of virus pill usual has a kind of nucleus acid. After virus infect sensitive host cell, the nucleus acid of virus will enter cell and produce its descendant and new protein by reproduction and expression. Then, the new synthetic virus is made up of descendant that release form cell by regular way. This specific way of reproduction of virus called reproduction.

病毒不具有细胞结构，一些简单的病毒又由核酸和蛋白质构成，故可把它们视为核酸蛋白分子，一种病毒的毒粒内通常只含有一种核酸，病毒感染敏感宿主细胞后，病毒核酸进入细胞，通过其复制与表达产生子代病毒基因组和新的蛋白质，然后由这些新合成的病毒组分装配成子代毒粒，并以一定的方式释放到细胞外，病毒的这种特殊繁殖方式称为复制。

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇／卵磷脂／十八胺脂质体包裹，免疫2周龄SPF鸡，4周后用同型禽流感病毒进行人工感染，1周后采集泄殖腔分泌物分离病毒，结果未免疫组6／6分离到病毒，裸质粒DNA免疫组1／6分离到病毒胆固醇／卵磷脂／十八胺脂质体包裹DNA免疫组1／6分离到病毒，DC-chol脂质体DNA免疫组没有分离到病毒（0／6）：人工感染后1周各组的HI抗体（Log2）分别为6.38±0.98，7.00±1.26，7.83±1.17，8.00±0.89，2周后为8.50±0.55，8.67±0.82，8.68±0.45，9.33±0.52，脂质体包裹组在同期均高于未免疫组和裸DNA免疫组，表明脂质体包裹质粒DNA免疫动物后，能增加动物对病毒感染的抵抗力和反应能力。

In order to improve the viral detection method in shrimp, a more rapid and convenient multiplex reverse transcription-polymerase chain reaction was established in the present study. The new method was used for detection of shrimp viruses, including Yellow-head virus, white spot syndrome virus, Taura syndrome virus, hepatopancreatic parvovirus, infectious hypodermal and hematopoietic necrosis virus and monodon baculovirus.

为了提高对虾病毒检测的快速性和简便性，建立了一种可同时检测6种对虾病毒的RT-PCR方法，并利用该方法对来自广西沿海地区的130份病虾样品中的黄头病毒、白斑综合征病毒、桃拉病毒、肝胰腺坏死病毒、传染性皮下组织坏死病毒以及杆状病毒进行了检测。

In general, overall nucleotide sequence identity between two distinct viruses are less than 89%, and strains of virus must have sequence identity greater than 89%, so VA2 is a distinct begomoviruses. In accordance with the revised proposal for naming geminiviruses, we propose to name VA2 as Malvastrum yellow vein virus.

根据&双生病毒科病毒全基因组核苷酸序列同源性小于89％，往往定名为不同病毒；大于89％，则认为是同一病毒的不同株系&这一原则，VA2为双生病毒的一种新种，根据病毒分类委员会命名原则，我们将该病毒命名为赛葵黄脉病毒，英文名为Malvastrum yellow vein virus，简称MYVV。

Methods The serum of 187 children with pneumonia from July 2002 to December 2004 in hospital were investigated for respiratory syncytial virus, adenovirus 3 influenza viruses A and B, parainfluenza virusestype 1,2,3,4 and coxsackievirus A 1.7, echovirus 7 by employing the indirect immunofluorescence assayfor the identification of nearly 8 different viruses, and 3 different enteroviruses.

采集2002年7月~2004年12月因肺炎住我院儿科的187例患儿血清，应用间接免疫荧光法检测8种常见呼吸道病毒病原体和3种肠道病毒的抗体：呼吸道合胞病毒、腺病毒3(ADV3)、流感病毒A和B型、副流感病毒l~4型、柯萨奇病毒A1、7型、埃可病毒7型的特异型IgM抗体。

Results The highest positive rates were found in respiratory syncytial virus (RSV,30.5%) and adenovirus (AdV,27.4%),and the other findings were as following: Coxsackie virus (CV,11.3%), EB virus (EBV,6.5%),echovirus (ECHOV,4.8%),parainfluenza virus (PIV,1.6%), influenza virus B (IVB,1.6%) and influenza virus A (IVA,0%).

结果 呼吸道合胞病毒、腺病毒阳性率最高，分别为30.5%、27.4%，其次为柯萨奇病毒、EB病毒，分别为11.3%、6.5%，埃可病毒为4.8%，副流感病毒及流感病毒B均为1.6%，流感病毒A为0%。

Based on the conserved region nucleotide sequences of four viruses (Alfalfa Mosaic Virus, ALMV; Cucumber mosaic virus, CMV; Cucumber mosaic virus-satellite, CMV-sat; Potato virus Y, PVY) and one viroid (Potato spindle tuber viroid, PSTVd) which could be pathogenic on potato world and one inner control(18S rRNA), specific oligonucleotide probes and primers were designed and spotting for hybridization detection.

利用芯片点样仪将5种侵染马铃薯的病毒/类病毒(苜蓿花叶病毒、黄瓜花叶病毒、黄瓜花叶病毒-卫星病毒、马铃薯病毒Y、马铃薯块茎纺锤状类病毒)的保守区寡核苷酸(Oligonucleotide，oligo)探针和PCR探针点样于玻片，并以植物18S rRNA作为内参照制成基因芯片。

Abstract］ Objective For the purpose of scientific evaluation and improvement on unreasonable parameter of viral inactivation,the dynamics curve of time corresponding to effect by given viral inactivation factor treated to blood plasma product were analyzed.Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.The mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.

目的 分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参方法系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低pH常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低pH/巴氏消毒法；人纤维蛋白原、人凝血因子Ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。