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After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including ESTss analogous to 11.5KD antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. Primers designed according to the sequences were used to amplify the corresponding ESTss from both blood and cephalothorax cDNA library.

在仔细分析了它们在免疫系统中的重要性和在对虾中出现的可能性之后,从中选出了17条可能编码抗菌肽,溶菌酶,凝集素、丝氨酸蛋白酶及其抑制剂,抗冻蛋白等蛋白质的序列,以此为依据设计引物,在中国对虾的血液和头胸部cDNA文库中扩增相应的序列。

However, this is stili a challenge in the Chinese shrimp :one reason is the large chromosome number of F.

本研究首次将功能基因定位在了对虾染色体上,为中国对虾的遗传物理图谱的构建提供了有效可行的方法。

Chinese shrimp is one of the most important economically valuable crustacean in China. Due to the limited knowledge on this system, eyestalk ablation is still a main method to promoting gonad development and maturation.

中国对虾是我国重要经济甲壳动物,由于缺乏对中国对虾这一系统的了解,至今仍采用切除眼柄的促进性腺发育、成熟。

Based on the findings in the comparative study of immune parameters between Chinese shrimp Fenneropenaeus chinensis and Kuruma prawn Marsupenaeus japonicus, this thesis firstly carry on the researches of iNOS activity in shrimps, and find it a reliable parameter for indicating the disease resistance ability in shrimps. The results here could offer a sound scientific basis for the better understanding of the mechanism of shrimp disease resistance ability to WSSV infection, as well as the development of countermeasures for the disease control in shrimp culture industry.

本文在系统比较两种对虾感染WSSV病毒过程中抗病力指标变化的基础上,优选出与WSSV感染直接相关的抗病力指标,并首次对对虾的一氧化氮合成酶进行了系统的研究,所得到的结果为深入了解对虾对白班综合症病毒的抗病力机制,并建立和发展相应的病害防御措施提供了科学依据。

Furthermore, the iNOS was identified in the haemocytes of Chinese shrimp F. chinensis and Kuruma prawn M. japonicus, and the methods developed were employed to evaluate their immunocompetence to WSSV infection in shrimps.

在对现有文献进行全面综述的基础上,我们通过实验系统比较了中国对虾和日本对虾在摄食感染WSSV后的死亡率、免疫学因子和感染病毒的变化情况,以及在氨氮胁迫下对虾感染WSSV过程中免疫学因子的变化情况。

Methods After the sample of Fenneropenaeus chinensis, Litopenaeus vannamei, Liza haematocheila, Acanthogobius hasta, Mactra veneriformis, Aloidis laevis, Ostrea denselamellosa and Fenneropenaeus chinensis was digested with concentrated hydrochloric acid, the lead content in marine products was measured by fluorimetric method and the results were compared with dithizone spectrophotometry.

采用湿式消解法对中国对虾、南美白对虾、梭鱼、矛尾刺虾虎鱼、四角蛤蜊、光滑兰蛤、密鳞牡蛎、中国对虾样品进行处理,采用浓盐酸作溶剂,荧光分光光度法直接检测铅含量,并与双硫腙光度法的测定结果进行比较。

The results showed that most of the microbes could be inactivated for the ultra high pressure treatment, the accumulation of total volatile basic nitrogen was restrained, and the change of pH value was delayed. The shelf life of white shrimp was prolonged with the enhancement of high pressure. High pressure treatment could make fresh shrimp with boiled flavor in some degree. Trements of 400 Mpa and 600 MPa aggravated blackening. However, melanotic reaction was not observed during the whole storage of shrimp treated by 700 MPa. High pressure could change the metabolizing of ATP and its metabolites, but did not impact the metabolic pathway of AMP decomposition.

结果表明:超高压处理可以有效地杀灭南美白对虾中绝大多数微生物,抑制贮藏过程中挥发性盐基氮的积累,延缓pH值的变化,从而延长南美白对虾的货架期,且处理压力越高延长效果越显著;超高压处理会给鲜虾带来不同程度上的煮熟虾的风味;400 MPa和600 MPa处理使虾在冷藏过程的黑变提前,而700 MPa处理可以完全抑制南美白对虾黑变现象的发生;超高压能够改变腺苷三磷酸及其代谢产物的代谢情况,但不影响腺苷酸的代谢途径。

The comparative experiments of rearing Penaeus vanname larvae fed the microencapsulated diets and micro-partical diets of "OK" brand and a searies of microgranular diets of "Bonasse" brand was performed. Two brands of diets are respectively formulated with rotifera at percentage of 70%, 80%, 90% and 100%. During the nursery period, the water quality factors such as the concentrations of NH3-Nt, NH3-Nm, NO2-- N, COD and main planktons were tested to find out their change regulation and the influence on survival rates and metamorphic rates of shrimp larvae.

本文利用欧开牌微胶囊饲料、微粒饲料和博尚牌系列微型饲料作为对虾育苗饲料,分别以70%、80%、90%和100%的比例与卤虫活饵料配合来培育凡纳对虾幼体,通过对凡纳对虾幼体有关生长发育指标的对比以及对育苗期间水中总氨氮(NH_3-N_t)、非离子氨氮(NH_3-Nm)、亚硝酸盐氮(NP_2~--N)、化学耗氧量和主要浮游生物等水质因子进行检测,找出它们的变化规律并比较上述饲料对幼体生长发育、成活率、变态率和育苗效果的影响。

Sequence analysis showed FCproPO contained two conserved copperbinding sites, Phylogenetic analysis revealed that FCproPO and prophenoloxidase from L. vannamei, M. japonicus and P. monodon were in the same phylogenetic branches.

序列分析发现FCproPO含有两个保守的铜离子结合位点;进化分析发现FCproPO与斑节对虾、日本囊对虾、凡纳滨对虾等海水虾的酚氧化酶原亲缘关系最近。

In the stage of prawn culture, twenty-three predator fishes were found. They are chiefly composed of such species as Portunus trituberculatus, Nibea albiflora, Eleutheronema tetradactylum, Lateolabrax japonicus, Triclentiger trigonocephalus.

对虾池内基础饵料种类和对虾养成期各阶段虾池及附近海域危害对虾敌害生物调查,目前未见详细报导。1983~1984年,我们在普陀朱家尖对虾养殖场和定海北蝉大成虾场进行了初步调查。

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