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Loose carapace and swollen cephalothorax were likely characteristic symptoms of white spot syndrome. There was probable relation between these symptoms and the reproduction of WSSV in penaeid shrimps.

甲壳下水肿和甲壳易剥离可能是斑节对虾白斑症的特征性病症;对虾外观病症的出现可能与对虾体内病毒的增殖相关联。

Results: The infected penaeid shrimps showed a rapid reduction in food consumption, lethargy, a pink to reddish-brown coloration and a loose carapace with white spots in succession, followed by ultimate death. The characteristic features of moribund shrimps were empty stomach, a pink to reddish-brown coloration, white spots on a loose carapace and swollen cephalothorax. The relative content of WSSV DNA in blood increased rapidly after the infected shrimp showed its first symptom of detestation of food.

结果:斑节对虾感染白斑症病毒后,依次表现出厌食、不活动、体色变红、甲壳出现白斑、反应迟钝直至侧卧死亡;濒死对虾的典型症状为空胃、红体、甲壳白斑、甲壳下水肿及甲壳易剥离;感染对虾出现拒食后,其血液中白斑症病毒 DNA的相对含量急剧增加。

The taste of cultured prawn extracts was slight sweeter and less flavorful than that of wild prawn extracts.

以感官法评价两类对虾提取物,得到结果是养殖对虾尝味稍甜,鲜味比天然对虾的差。

The random amplified polymorphic DNA and inter-simple sequence repeat analysis were used to estimate population genetics of Fenneropenaeus chinensis. We analyzed the genetic diversity and genetic structure of the populations, and also studied the genetic divergency among populations and geographic populations.

本文以中国对虾野生群体为研究对象,采用RAPD和ISSR分子标记技术,进行了群体遗传学的研究,对中国对虾野生群体的遗传多样性及群体遗传结构进行了分析,研究了中国对虾各野生群体之间的遗传分化情况。

Result The heterobacteria, vibrios and pathogenic Vibrio parahaemolyticus were the roost in fertilized eggs of shrimp rot the least in nauplius, then their number would increase with growth. During whole rearing period, both heterobacteria in larvae, vibrios in water would increase by one order of magnitude, while both vibrios in larvae and heterobacteria in water would increase by two orders of magnitude.

结果]异养菌、弧菌和致病性副溶血菌数量都是对虾受精卵中高,无节幼体中最低,而后逐渐升高在整个育苗期,对虾幼体中异养菌和养殖水体中弧菌增加1个数量级,对虾幼体中弧菌和养殖水体中异养菌均增加2个数量级。

Through measuring their ability to degrading shrimp feed, 10 functional bacteria strains were obtained,which are capable of utilize shrimp feed as the sole source of carbon and nitrogen,degrading artificial shrimp feed and clam rapidly(CODMn removing rate are over 60% and 70% in 72hours,respectively),and growing well in shrimp culture environment.

通过测定其对对虾饵料降解能力,筛选到虾池有机污染物降解菌10株,这些菌能以对虾饵料为唯一碳源和氮源生长,对对虾配合饵料和蛤蜊肉具有较强降解能力(72 h内 CODMn去除率分别在60%和70%以上),并可在虾池环境条件下良好生长。

Result The climate in Jiangsu's littoral was suitable for the farming of Penaeus vannamei, Penaeus chinensis, Penaeus monodon and Penaeus japonicus.

结果]江苏沿海的气候适宜养殖南美白对虾、中国对虾、日本对虾、斑节对虾

Using the on-line sequence similarity searching tool Fasta3 on internet web,many neuropeptides from crustacean CHH family of high similarity with thesededuced amino acid sequences from cDNA of eyestalks from these four kinds animalswere found.

在所有的相似序列中,分别由中国对虾、中华绒螯蟹、鹰爪虾和蓝对虾的特异性cDNA片段推定的氨基酸序列与蜕皮抑制激素的相似度最高,这一结果提示分别由中国对虾、中华绒螯蟹、鹰爪虾和蓝对虾的眼柄特异性cDNA片段推定的氨基酸序列可能是这四种甲壳动物的MIH片段。

The results indicated that compared to the control group, the total bacterial counts in the shrimp intestine remained constant,while Vibrio sp.numbers decreased significantly,and the total haemocyte countand PO activity in the shrimps increased significantly,SOD activity increased significantly only when water was supplemented with B.licheniformis S-40 at 10~5 cfu·mL~(-1).However,the protein contents were not remarkably different between the experimental and control groups.

结果显示:与对照组对虾相比,所有实验组对虾消化道的弧菌数有显著下降,而总菌数没有呈现显著性差异;所有实验组中对虾血淋巴上清液的血细胞数,酚氧化酶活性均有显著性上升,超氧化物歧化酶仅在10~5cfu/ml时有显著上升,菌剂的加入对对虾血淋巴上清液的的蛋白浓度并无显著影响。

The results showed that an incubation time of 8 hours is most suitable for the analysis of iNOS enzyme activity. The four methods could identify iNOS in shrimp haemocytes and analyze the activity effectively, thus provide a good way for the further study of role of iNOS in innate immunology of shrimps.

本文针对这一问题,系统研究了对虾在白斑综合症病毒感染和环境因子胁迫下先天性免疫因子的动态变化过程,筛选出了能够准确反映对虾健康状况的免疫因子,进而将其运用到对虾抵抗WSSV的机制和环境胁迫对对虾健康状况的影响的研究中。

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