对虾

Shrimp white spot bacilliform virus, also called white spot syndrome virus, is the heaviest pathogen to shrimp culture.

对虾白斑杆状病毒(White spot bacilliform virus WSBV)，或称白斑综合症病毒，是对虾养殖业最主要的病原。

After feeding of 20 d, 40 d and 60 d, the activities of nitric oxide synthase, bacteriolytic, superoxide dismutase, alkaline phosphatase and acid phosphatase in the serum were determined and the relativities between them were also analyzed.

在饲料中添加不同剂量的嗜酸小球菌投喂凡纳滨对虾，并在投喂后的20、40和60d，分别测定凡纳滨对虾血清中的一氧化氮合酶、溶菌活力、总超氧化物歧化酶、酸性磷酸酶、碱性磷酸酶，并分析了这5种酶之间的相关性。

There was a plus relationship between NOS and bacteriolytic activity and a minus relationship between NOS and SOD as well as a minus relationship between bacteriolytic activity and AKP, which indicated that feeding Pediococcus acidilactici could significantly increase the activities of the immune factors in the shrimp body.

表明在饲料中添加适量的嗜酸小球菌（剂量10mg/kg）可使凡纳滨对虾体液免疫因子活力到达较高的水平；凡纳滨对虾血清中一氧化氮合酶对于嗜酸小球菌的添加量较为敏感，可作为评价嗜酸小球菌使用效果的指标之一。

In this study, copepoda and its resting eggs in shrimp culture ponds were detected using PCR dot-blot hybridization and nest-PCR for the presence of WSSV. The results showed that WSSV positive cases were detected in copepoda from egg to adult, and the positive copepoda appeared earlier than cultural shrimp in ponds.

1。本研究利用PCR-斑点杂交和巢式PCR法检测野外发病对虾养殖池塘中桡足类及其休眠卵，以及由这些休眠卵孵化出的幼体携带病毒情况，发现桡足类由卵到成体各阶段中均有阳性个体，而且池塘中在春天放养对虾苗前桡足类就出现阳性个体。

We have developed a method for detection of Hypodermal and hematopoietic necrosis baculovirus by polymerase chain reaction.

皮下和造血器官坏死杆状病毒［1］、对虾杆状DNA病毒［2］和白斑杆状病毒［3］是近年来引起全球对虾暴发性死亡的病原。

In order to improve the viral detection method in shrimp, a more rapid and convenient multiplex reverse transcription-polymerase chain reaction was established in the present study. The new method was used for detection of shrimp viruses, including Yellow-head virus, white spot syndrome virus, Taura syndrome virus, hepatopancreatic parvovirus, infectious hypodermal and hematopoietic necrosis virus and monodon baculovirus.

为了提高对虾病毒检测的快速性和简便性，建立了一种可同时检测6种对虾病毒的RT-PCR方法，并利用该方法对来自广西沿海地区的130份病虾样品中的黄头病毒、白斑综合征病毒、桃拉病毒、肝胰腺坏死病毒、传染性皮下组织坏死病毒以及杆状病毒进行了检测。

Chinensis and Kuruma prawn M. japonicus increased at 12 and 48 hours post-inoculum, but decreased sharply after 36 and 72 hours post-inoculum respectively. LPS inducible NOS activity also decreased significantly with the time elapsed in WSSV infected shrimps. Results of WSSV detection with the Dig-DNA Probe dot blot hybridization test showed that WSSV of the two penaeid species couldn't be detected until iNOS started decreasing.

在此基础上，我们建立了对虾血细胞中诱导型一氧化氮合成酶的鉴定方法—硝基四氮唑蓝还原法和血细胞形态法以及酶活力的测定方法—L-瓜氨酸分析法和亚硝酸盐分析法并优化了鉴定和测量条件，结果显示：对虾血细胞在4℃下与L-精氨酸和LPS孵育8h以及当L-精氨酸浓度为2.5mmol l〓LPS浓度为100μg ml〓，Ca〓浓度为2.5mmol l〓时，iNOS活力的测定结果最佳。

The experiments were conducted to find out the effects of calcium, copper, zinc, manganese ions, SDS and EDTA (ethylene diamine tetraacetic acid) on the superoxide dismutase in muscle of kuruma shrimp Penaeus japonicus, prawn Procambarus clarkii and Bacific white shrimp Litopenaeus vannamei and the differences in SOD in muscle, gill and hepatopancreas.

测定了Ca(上标 2+)、Cu(上标 2+)、Zn(上标 2+)、Mn(上标 2+)及SDS、EDTA不同浓度对日本对虾、克氏原鳌虾、凡纳滨对虾肌肉组织中超氧化物歧化酶活性的影响及其肌肉、鳃、肝胰腺组织中SOD活性的差异，并利用电泳技术研究了组织同工酶。

In order to study the effects of seawater physical and chemical factors on the mean survival time of WSSV-infected P. monodon and L.

本文通过人为改变养殖海水的单项或多项理化因子，研究这些改变对感染WSSV的斑节对虾和凡纳滨对虾的平均存活时间的影响。

By applying the method of mRNA differential display to normal and disease-resistant shrimp Penaeus monodon, 9 differential bands were observed.

通过荧光mRNA差异显示技术，对正常斑节对虾和抗病斑节对虾进行分析，获得了9个差异cDNA片段。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。