基因

Firstly, we cloned a newβ_2-tubulin gene from the macronuclear andmicronuclear geneome of E. octocarinatus (DQ864704), respectively.

本研究首先克隆了八肋游仆虫的β_2-微管蛋白新基因(DQ864704)，与β_1-微管蛋白基因相比，基因编码区(1335bp)的同源性达到99.1％。

Accordingly, we studied the influence of BmIce in apoptosis of BmE-SWU1 through Hoechst dying.The main results are as follows:1 The analysis results of silkworm genome chip dataThe genome chip data in different silkworm tissues indicated that at the third day instar 5, the highest expressive quantity of BmIce appeared in intestine, the second highest one appeared in blood, and the lowest one in Malpighian tubes.

此外，利用家蚕全基因组芯片数据，分析了BmIce基因表达谱，并对BmIce基因在不同因子诱导下家蚕BmE-SWU1细胞中的表达量进行了荧光定量PCR分析，同时对不同表达量的BmIce基因对BmE-SWU1细胞凋亡的影响进行了Hoechst核染观察。

To acquire more information about gene expression at the whole-genome scale,our lab and National Engineering Center for Beijing Biochip Technology worked together and constructed the first genome-wide oligonucleotide microarray of the silkworm.This microarray was used to detect gene expression of 10 tissues/organs,including midgut,integument,head,hemocyte,testis,ovary,anterior/median silk gland,posterior silk gland,fat body and malpighian tubule.In this study,we performed analysis of these microarray data and confirmed the reliability of the results.

为了在全基因组水平上获得基因表达信息，本实验室与生物芯片北京国家工程中心合作，共同定制完成了世界上第一张家蚕全基因组寡核苷酸基因芯片，并利用该芯片对家蚕5龄第3天幼虫的中肠、体壁、头、血液、精巢、卵巢、前中部丝腺、后部丝腺、脂肪体、马氏管这10个组织中的基因表达谱进行了检测。

Objective: The genes were connected basing on obtain gene of MCP and DAF of liver and fetal marrow of Chinese adult and then design mammiferous vector of the hMCP-DAF fusion gene. Finally transfer hMCP-DAF fusion gene to the early embryo of jinhua''s pig.

目的 在获得中国成人肝脏、胎儿骨髓MCP、DAF基因克隆的基础上，进一步将两个基因串接，并构建hMCP-DAF融合基因的哺乳动物表达载体，筛选出具表达活性的hMCP-DAF并导入金华猪早期胚胎。

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

Objective: To study the effect of the mutant p27 gene （p27mt gene） on growth of the colorectal cancer cell line SW480 and to evaluate the therapy significance by DNA ploidy analysis and apoptosis measuration.

目的：观察人突变p27基因（p27mt）对人大肠癌细胞生长的影响，探讨p27mt基因在大肠癌基因治疗中的作用机制。

Among all, deleted in liver cell (DLC-1) gene was found to play an important role in hepatoma in 1998, and after that hypermethylation on the promoter of the DLC-1 gene was found in many other cancers, suggesting that epigenetic modification is another way to control the carcinogenesis. Another gene, Endothelin type B receptor, was found to cause megacolon syndrome by accident while searching the cause of white spot in mouse, and was also found hypermethylation on its promoter in some cancer cells.

在此中，DLC-1基因自从1998年后被发现在肝癌的演化上扮演重要的角色后，在许多其他的癌症上也发现此基因上位於promoter的CpG islands有过度甲基化的现象，推论DLC-1基因的过度甲基化是一种经由epigenetic modification调节细胞癌化的方式。

The morphologic and immunohistochemical discrepancies between this intriguing melanotic tumor and other documented renal cell carcinomas bearing identical PSF-TFE3 gene fusion may suggest melanotic Xp11 translocation renal cancer is a distinct entity among the MiT/TFE family neoplasms.

与同样发生PSF-TFE3基因融合的其他已知肾细胞癌相较，此例的形态、免疫表型差异可以说明，黑色素性Xp11基因易位肾癌是MiT/TFE基因家族肿瘤中一个独特的实体。

Both honey bees and bumble bees can be infected by microsporidia, and the researches about the rRNA of microsporidia are mainly concentrated on sequencing the rRNA, the secondary structures of rRNA and detection and diagnosis of microsporidia by rRNA gene.

蜜蜂和熊蜂都被微孢子虫感染，关于蜜蜂和熊蜂微孢子虫rRNA基因研究主要有rRNA基因序列的测定分析、二级结构的研究和用rRNA基因检测诊断微孢子虫等。

In 1980s, molecula biology technology had been applied to familial diabetic genetics, a suit of single genes about diabetes was found: insulin gene, insulin receptor gene and so on.

自上个世纪80年代以来，应用分子生物学技术进行家族性糖尿病遗传学研究发现了一系列与糖尿病相关联的单基因，即胰岛素基因和胰岛素受体基因等。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。