基因

Methods: Clone the genes of ScFv (OC183B2) and PE38 into PET30a and then express the immunotoxin after induced with IPTG.

采用基因工程技术，经过3次亚克隆，将编码单链抗体的基因及编码毒素的基因亚克隆至原核表达载体pET30a上，并在IPTG的诱导下进行表达。

Overexpression of MDR1, GST-π, p53 mRNA and their corresponding proteins in epithelial ovarian cancer participated in the formation of multidrug resistance in ovarian cancer and P53 may play a indirected role through regulating the expression of MDR1、GST-π.

卵巢癌化疗耐药与耐药基因GST-π、MDR1过表达及细胞凋亡相关基因P53突变密切相关，突变的P53可能通过调控耐药基因GST-π、MDR1而间接发挥其耐药作用。

S inger argues to prenatal geneti c screening and gene selection to presume germ-line cell gene therapy.

基因治的种类及消极、积极基因治疗的界线问题，基因增强、优生学也是我们的重点。

Japonica sinensis to the putative ancestral pancrustacean (crustacean/insecta) gene order.

中华绒螯蟹线粒体基因组与推测的泛甲壳类原始的线粒体基因顺序相比，至少有12个基因发生了重排，造成线粒体基因排列顺序显著的变化。

Rearrangements have been occurred in at least twelve mitochondrial genes, which have drastically altered the gene order of the mitten crab E. japonica sinensis to the putative ancestral pancrustacean (crustacean/insecta) gene order.

中华绒螯蟹线粒体基因组与推测的泛甲壳类原始的线粒体基因顺序相比，至少有12个基因发生了重排，造成线粒体基因排列顺序显著的变化。

Objective: Fibrinolytic enzyme gene of Gloydius intermedius was cloned,then analysis the gene sequence and function.

目的：克隆中介蝮蛇纤维溶解酶（Fibrinolytic enzyme，FLE）基因，分析其基因序列并对该基因的功能进行分析。

Fibrinolytic enzyme gene of Gloydius intermedius was cloned, then analysis the gene sequence and function.

克隆中介蝮蛇纤维溶解酶(Fibrinolytic enzyme， FLE)基因，分析其基因序列并对该基因的功能进行分析。

According to the results using near isogenic lines, durable disease resistance variety can be bred by introducing a strong resistance gene together with 2 to 3 other resistance genes.

依据对堆叠抗病基因近同源系的抗性分析，可得知导入一个抗性较高的基因，并搭配2至3个抗病基因，即可达成耐久抗性的目标。

To study the gene expression profile on nasopharyngeal carcinoma,lung cancer and normal adult nasopharynx tissue and to obtain the NPC related gene, the total RNA extracted from these tissues were retro transcripted and labeled with α 32 P isotype.

研究鼻咽癌、肺癌与正常鼻咽组织基因表达谱差异及筛选鼻咽癌相关基因，采用α32P逆转录标记组织总RNA，将cDNA 探针与有5 184 个基因或表达序列标签EST 的高密度cDNA 微阵列GF200 杂交，软件分析表达谱差异。

Finally, taking these dates and using it as the functions of genetic program, and using the standard arithmetic operation as the terminals of GP. The object in this algorithm is the chiller consumption energy. Through the option of GP which contains 'crossover'、 'reproduce' and 'mutation' to iterant compute the population is composed of functions and the terminals of GP. Finally, we would find the consumption energy model of chiller.

利用此三参数作为基因规划法之终点端，再利用数学符号作为基因规划法之函数端，以实际之冰水主机耗电量作为目标，利用基因规划法之运算子作迭代运算，最终可得到足以说明此三参数与冰水主机耗电量之关系函数，即为冰水主机耗电模型。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。