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Gl_2~e is a gene controlling glandless traits in cotton plant and seed, which is an important gene resource for gossypol-free cottonseed breeding.

Gl_2~e基因是控制棉花植株和种子无腺体的一个基因,是低酚棉育种中一个重要的基因资源。

The Lyb genes were novel members of Y-box gene family containing a conserved cold-shock domain with two RNA binding motifs (RNP-1 and RNP-2). Moreover, phylogenetic analysis revealed that there was only one type of Y-box gene before the emergence of gnathostomata. By contrast, the Y-box gene were divided into three groups, YB1, YB2, and CSDA during evolution of jawed vertebrates.

此外, 从Swiss-Prot下载了经实验验证过的Y-box蛋白序列数据,结合实验克隆得到的Lyb基因所编码的氨基酸序列构建系统发育树,发现Y-box基因在有颌类出现以前只有一种类型,有颌类出现以后, Y-box基因分化成YB1、YB2和CSDA三种类型。

Hoxall genes from 16 vertebrates were analyzed, containing 10 cloned Hoxall genes mentioned above and 6 Hoxall genes from GenBank, Homo sapiens, Mus musculus, Bos taurus, Polyodon spathula, Heterodontus francisci, and Oryzias latipes, in representatives of the three major gnathostome lineages: cartilaginous fish, ray-finned fish and lobe-finned fish.

克隆的基因与从GenBank中获取的人、小家鼠、黄牛、匙吻白鲟、角虎鲨Hoxa11基因及青鳉Hoxa11a基因进行比较分析,这些动物代表了有颌类的三个谱系:软骨鱼类谱系、辐鳍鱼类谱系(斑马鱼、大黄鱼、花鲈、罗非鱼、红鳍东方鲀、乌鳢、青鳉和匙吻白鲟)和肉鳍鱼类谱系(矛尾鱼、热带爪蟾、绿背树蜥、家鸡、人、小家鼠和黄牛)。

Results BLASTn analysis revealed insertion or substitution of 18-38 nucleotides in the penA gene of gonococcal isolates with reduced ceftriaxone susceptibility.

将11株头孢曲松低敏和2株头孢曲松敏感的淋球菌penA基因基因测序,通过BLASTn与BLASTx分析,研究penA基因的碱基插入和置换情况及PBP2中氨基酸插入和置换模式。

The genus specificity was validated with 47 strains including wild type isolates. Preliminary screening of soil samples with Gordonia-specific primers was successful in the rapid detection of the presence of 36 Gordonia wild-type isolates.

经多序列分析比对软体计算并比较gyrB基因序列,可知Gordonia菌属与7种邻近放线菌的gyrB基因序列相似度介於72.8-82.1%,而在16S rDNA序列相似度则介於90.0-95.4%,gyrB基因可明显区分Gordonia菌属与其他属之放线菌。

In this study chitinase and β-1,3-glucanase were transferred into Gossypium hirszrtum CCRI 24 by Agrobacterium tumefaciens .

本试验利用根癌农杆菌介导的转基因方法,将双价抗真菌病基因几丁质酶基因和β-1,3-葡聚糖酶基因导入棉花中棉所24中。

Graminaceae ; Tissue culture: Regulation of gene expression ; Micropropagation ; Gremplasm conservation ; Crop breeding ; Somatic hybrization ; Gene transfer techniques ; Plant genie engineering

禾本科植物;组织培养;基因表达的调控;无性系繁殖;种质保存;作物育种;体细胞杂交;基因转移技术;植物基因工程

Before and after treatment, 5 lines displayed GUS expression only before treatment, and 16 lines displayed

表达的水稻基因启动子及其基因,并进而对水稻基因的表达特征与功能进行研究。

The predicted amino acid sequence of the gene show a rather high homology(84.47% amino acid identities)with Haemaphysalis longicornis(H.longicornis) TnI (GenBank,GI:14041807).Furthermore, the actin-binding domains of troponin I in predicted amino acid sequence, just the same as H. longicornis ,at the amino acid residues 147-167, suggesting the gene is K haemaphysaloides TnI gene.

经同源性比较,该基因预测的氨基酸序列与长角血蜱肌钙蛋白Ⅰ(GenBank,GI:14041807)有很高的同源性(同源性为84.47%),肌动蛋白结合位点位于147-167氨基酸处,且与长角血蜱肌钙蛋白Ⅰ肌动蛋白结合位点完全相同,表明该基因是镰形扇头蜱肌钙蛋白Ⅰ基因

Objective To construct spinocerebellar ataxia 3 (SCA3) eukarotic expression vector with en hanced green fluorescent protein as report gene and to observe its expression in PC12 cells.

目的 构建以增强型绿色荧光蛋白作为报告基因、带有脊髓小脑性共济失调3型(SCA3)基因的真核细胞表达载体,并转染PC12细胞,观察外源基因的表达情况。

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