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Then the fusion protein was primary purified by affinity chromatography. The GST tail of the fusion protein was cleaved by Thrombin. Nucleotide sequence analysis showed that the gdh gene of S.suis possesses the highly conserved motifs typical of family I hexameric GDHs.

猪链球菌2型谷氨酸脱氢酶编码基因克隆、序列分析及原核表达根据已发表的猪链球菌2型的gdh基因设计引物,扩增海安病人分离株Habb中的目的基因,并进行序列测定和分析;构建重组表达载体pGEX4T-2-gdh,在大肠杆菌中表达,并纯化重组蛋白。

Plant homeotic genes and homeobox genes are two types of the important genes encoding transcription factors involved in plant development.

植物同源异型基因及同源异型盒基因是涉及植物个体发育调节的两类重要转录因子编码基因

Although the study of a series of genes including the homeotic family has yielded remarkable insight into the molecular mechanisms underlying the development of brain regarding both human and related model organisms, a great number of novel genes remain to be investigated.

虽然含同源异型盒的基因家族等一系列基因在胚胎脑组织的表达特征及有关功能已被阐明,迄今为止,大量与人脑发育、分化相关的基因尚属未知。

A new gene structure is proposed: head+body+tail, which allows the program with necessary complexity and putting some learning mechanism into the search process.② A new homeotic gene structure is proposed, it not only can call for subroutines easily, but also can automatically perform programming.③ The concept of different homeotic gene, a multi-cellular structure is proposed. It can be used to describe the complex multi-level programs and to implement the complex subroutine calls.④ An estimation of distribution operator for guiding search is proposed. It fuses statistic learning mechanism into the search process to accelerate the convergent process and improve the quality of solutions.

提出了一种新的基因结构:头+身+尾,使计算机自动设计的程序具有必要的复杂性,又便于引入学习机制;②提出了一种新的同源基因结构,它不仅可实现子程序的调用,还具有很强的编程能力;③提出了异族同源基因的概念:一种多细胞结构,它能描述复杂的多层次程序结构,实现可重用程序的复杂调用;④提出了分布估计变异方法,将统计学习机制融入算法,既提高了算法的收敛速度,又提高了解的质量。

Because homeotic gene mutation and floral organ variation is a direct relationship,therefore the mutant application is the current functional genomics study developmentdirection. We discovered one mutant ah from line 458, which was a line ofthe twin-seedling strain W2555.ln this study, we have investigated the morphogenesisprogress, genetic analysis of mutant traits and molecular tagging of related gene.

目前,由于在水稻中发现的花器官突变体很少,所以对水稻花发育的研究主要是利用双子叶植物MAS-box基因的保守序列设计引物,在水稻基因组或者cDNA文库中筛选与双子叶植物花器官发育相对应的MADS基因,因为同源异型基因的突变与花器官的变异是直接对应的关系,所以突变体的应用是当前功能基因组学的发展方向。

Inaddition to that,LFY has a second role in the activation of the floral homeotic genes that specify the identity of organs in the flower and is a key intergrator of the outputs of floral inductive pathways.DFL, a FLORICAULA/LEAFY homologous gene,which cloned from C.lavandulifolium,was a key gene of florescence for C.lavandulifolium.

LEAFY基因在植物成花过程中意义重大,它不仅决定着花由营养生长向生殖生长的转变,它还接连GA成花途径和长日途径的信号继而诱导花分生组织的转变,同时还是活化花器官基因的关键基因

The frequencies of CBS C homogenetic type (13.5%) and C allele (43.9%) in ischemic group were higher than those in control group (6.0% and 38.0%, respectively).

患者组CBS基因C纯合基因型和C等位基因频率分别为13.5%和43.9%,对照组分别为6.0%和38.0%。

Until now, the genes related to apoptosis of Bombyx mori which have logged in NCBI includes the BmP109, BmIap, BmCdc2, BmIce, BmIce2, BmIcad and 6G1 gene. Thereinto, the apoptotic function of the homologic genes of BmIce in Homo sapiens, drosophila and C.elegans have been identified. The aim of this study was to identify the function of the interleukin-1 beta converting enzyme of Bombyx mori, BmIce.

目前已在NCBI上登录克隆序列的家蚕细胞凋亡相关基因有:含Bcl-2家族同源域BH结构域的BmP109,凋亡抑制因子BmIap,生存因子途径中的BmCdc2,家蚕白介素转换酶BmIce和BmIce2,caspase活化的DNA酶抑制剂BmIead基因和30k蛋白中的6G1基因

Based on the degenerated primer, the cDNA of open reading frame of growth horme gene for black carp and Japanese crucian carp were cloned and sequenced through RT-PCR, which were used for the"all-fish"transgene construction and preparation of polyclonal antibody against GH for black carp.

基于鲤科鱼类生长激素基因序列,通过RT-PCR技术,克隆了青鱼、日本白鲫生长激素基因编码区cDNA序列,以此来构建&全鱼&基因载体和研制生长激素多克隆抗体。

Results In C6 cells, Hox genes expressed in different degrees. HoxB4 did not express, and HoxB2 expressed weakly.

结果:在星形细胞瘤C6细胞中, Hox基因存在不同程度的表达,HoxB4基因无表达,HoxB2基因弱表达。

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