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Results Beta-tubulin genes, 1439bp and coding for 443 amino acids, was acquired and was submitted to GenBank and to get an accession number that was AY220457. The beta-tubulin gene of S. japonicum and Fasciola hepatica were homologous with an identity of 79%.

结果 获得了1个日本血吸虫新基因-微管蛋白基因,全长1439bp,编码443个氨基酸,与肝片形吸虫微管蛋白基因具有79%的同源性。

ERAF17 gene expresses differently between male and female reproductive organs. Its expression is induced by ethylene or ethylene released compounds that can promote femaleness. No evidence directly shows the relationship between ERAF17 gene and femaleness, however. So this work focus on proving the positive correlation between the expression of ERAF17 gene induced by ethylene and development of female flowers,then search for the signal pathway of cucumber female reproductive organ development.

ERAF17基因在黄瓜的雌雄生殖器官的表达不同,可以由乙烯诱导表达,从而推动雌性化,但是它的作用机制却不清楚,并没有明确的证据表明ERAF17基因与雌花的发育有直接关系,所以本论文研究重点在于证明乙烯诱导ERAF17基因的表达对黄瓜雌花的分化有确定的正相关,从而可以探索黄瓜雌性器官发育存在的可能的信号通路,并对应用前景进行评估。

The adhesion test showed that small intestinal epithelium cells of 30-35-day-old postweaning piglets with both genotype M307~ and M307~ could adhere to the standard E.coli strain express F18ab fimbriae, the recombinant E.coli express F18ac rE.coli1534 and the recombinant pnirBMisL-fedF E.coli displaying FedF subunit on the surface of E.coli, but small intestinal epithelium cells with genotype M307~ and 3-day-old piglet could not adhere to the three kinds of bacteria descried above, the latter with good adherence capability of 987P fimbrial E.coli.

9仔猪小肠上皮细胞的黏附试验结果显示,30~35日龄M307~基因型和M307~基因型断奶仔猪的小肠上皮细胞均能与表达F18ab菌毛标准菌株107/86、诱导表达F18ac菌毛的重组大肠杆菌rE.coli1534及诱导菌体表面展示表达F18黏附亚单位FedF的重组大肠杆菌pnirBMisL-fedF发生黏附作用,而同日龄M307~基因型断奶仔猪的小肠上皮细胞均不能与上述三株大肠杆菌发生黏附作用。3日龄易感仔猪小肠上皮细胞也不能黏附上述三株大肠杆菌,但可以很好地黏附表达于987P菌毛的大肠杆菌。

The sequence alignment suggests that the secondary structures of these proteins are very similar to that of the Galanthus nivalis lectin and that they also possess the same sugar-binding site found in the GNA.

为研究天麻抗真菌蛋白基因的表达调控方式,构建了五个GAFP-2基因启动子区和GUS基因融合的表达载体,并通过农杆菌介导的植物遗传转化方法导入烟草中。

An expression vector was constructed with a safe marker gene, 6-phosphomannose isomerase, and an insect-resistant gene, Galanthus nivalis agglutinin, was used in rice genetic transformation in the present study.

采用农杆菌介导法将来源于大肠杆菌的6-磷酸甘露糖异构酶安全选择标记基因和雪花莲凝集素抗虫基因构建于同一表达载体上进行遗传转化,获得带有安全标记基因的抗褐飞虱转基因植株。

The results indicated that GFP gene expression began at the end of gastrula stage, and lasted to fry at a stable expression level.

采用显微注射法将报告基因GFP基因导入蓝太阳鱼受精卵中,研究了外源基因在鱼类胚胎发育过程中整合与表达的时序。

Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,and 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos.

测序和基因数据库比对结果表明,72个原肠期斑点杂交阳性克隆中,包括19个已知基因的cDNA片段和31个没有同源性的cDNA片段;98个尾芽期斑点杂交阳性克隆中,包括52个已知基因的cDNA片段和37个没有同源性的cDNA片段。

However, the mutation of GATA site at -2948bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. To test whether those sites act synergistically, we mutated 2 of these three binding sites with different combinations.

然而,GATAb(在-2948bp的GATA位点)的突变明显地降低了SV40启动子介导的报告基因转录活性,但是对ε-珠蛋白基因启动子介导的报告基因表达却影响不大。

One, the progress of the research of mankind of gene medicines and chemical reagents to oneself gene and gene project will be in will make pharmacy line of business obtains a leap in 10 years henceforth, remedial osteoporosis, early old sex fiber of sex of gawkish, bursa is out of shape the medicaments that waits for difficulty miscellaneous disease will come out, the cure of AIDS also will obtain a breakthrough.

一、基因药品人类对自身基因的研究和基因工程的进展将在今后10年中使制药业取得飞跃,治疗骨质疏松、早老性痴呆、囊性纤维变形等疑难杂症的药物将问世,艾滋病的治疗也将取得突破。

Our rapidly increasing understanding of the human genome and those of other organisms means that we are becoming capable of genetically modifying ourselves by cutting out ,rearranging,and adding various snippes of our own DNA molecules and implanting DNA swquences form other organisms.This secondary evolution will allow us to change the course and speed of primary evolution (based mostly on the glacially slow process of natural selection) of our own species and other species by creating types of genes not currently found in the rest of nature.

我们很快地增加了对人类基因组以及那些其他生物的了解的方法,我们成为有能力从基因方面修改我们自己的基因,藉由删去关掉、重新排列、以及增加各种的片段在我们拥有的DNA分子以及注入一连串的DNA序列从其他生物,而这第二个发展出来的东西将是允许我们藉由创造的类型(创造的类型:不是属於现在处於自然界中的基因)改变初始发展的过程及速度(初始发展:主要是建立在兵期的缓慢的自然淘汰的过程)在於我们拥有的种类以及其他种类。

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