基因

Fine mapping of Helminthosporium turcicum resistance gene Ht2 is extremely valuable for map-based cloning of the Ht2 gene,gaining a better knowledge of the distribution of resistance genes in maize genome and marker-assisted selection in maize breeding.

精细定位玉米大斑病抗性基因Ht2对于图位克隆该基因和探讨玉米基因组中抗病基因的分布规律及分子标记辅助选择有重要意义。

OBJECTIVE: To compare the differences of gene expression and the response to bFGF of human umbilical cord CD34+ and CD133+ cells, and to explore gene expression changes of bFGF-induced umbilical cord CD34+ and CD133+ hematopotic stem cells/hemapoietic progenitor cells in vitro.

目的：利用基因芯片比较脐血CD34+和CD133+细胞基因表达的差异，并探讨碱性成纤维细胞生长因子体外诱导脐血CD34+和CD133+造血干/祖细胞分化的基因表达变化，及其对碱性成纤维细胞生长因子反应性差异。

To investigate the clonal rearrangements of immunoglobulin heavy chain gene and T-cell receptor γ gene in peripheral blood cells of patients with hemapoietic malignancies and their significance, the clonal rearrangements of IgH and TCRγ genes were detected by polymerase chain reaction in peripheral blood cells of patients with hemapoietic malignancies, including 32 cases of non-Hodgkin lymphoma, 18 cases of acute myeloid leukemia, 24 cases of multiple myeloma, 8 cases of acute lymphoblastic leukemia and 6 cases of chronic lymphoblastic leukemia.

检测各种血液系统肿瘤患者外周血细胞免疫球蛋白重链基因和T细胞受体γ基因克隆性重排并探讨其意义。通过多聚酶链式反应方法检测32例非霍奇金淋巴瘤、18例急性髓性白血病、24例多发性骨髓瘤、8例急性淋巴细胞白血病及6例慢性淋巴细胞白血病患者外周血细胞IgH及TCRγ克隆性基因重排。

Most genes in the ABC model of the development of plant flowers in hermaphroditic plants belong to MADS-box genes. But the researches on MADS-box genes in dioecious plants are less.

雌雄同株植物花发育的ABC模型中已知的大多数基因都属于MADS-box基因，但在雌雄异株植物中对MADS-box基因的研究较少。

After the NK gene from recombinant plasmid pUC19-NK was cloned on expression vector pBV220, it was transformed into host cell E. coli HB101. Recombinant plasmid pBV220-NK was extracted for identifying by analysis of restriction enzymes, PCR and sequencing. Studies on recombinant strain's growth and expression showed that heterogene have no distinct effect on host cell. The recombinant plasmid have excellent segregational stability but low structural stability. The results of kinetics of pBV220-NK expression in E.

将重组质粒pUC19-NK上的纳豆激酶基因成功克隆到表达载体pBV220上，并将之转入大肠杆菌HB101中，得到转纳豆激酶基因工程菌，提取重组质粒经单酶切、双酶切及PCR分析验证后，对重组菌的生长及表达进行一系列研究，结果表明：外源纳豆激酶基因的导入对宿主的生长没有明显的影响：重组质粒的稳定性实验表明：该质粒具有良好的分离稳定性，而结构稳定性较差。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The higher percentage of cross-fertilized kernel specifically expressed pattern was observed in highly heterotic hybrid, after comparison of gene differential expression between cross-fertilized and self-fertilized kernels during the early development of seed in wheat and maize, respectively. This indicated that there may be have common molecular mechanism for heterosis in different crops.

本文对玉米和小麦在种子发育前期杂交和自交间基因差异表达进行了比较，发现杂交特异表达类型与杂种优势均存在一定的正向关系，说明不同作物杂种优势形成在分子水平上具有共同点，即相同表达模式的基因或基因群在不同作物杂种优势形成中发挥一致的作用。

It was shown that the resultant cybrid HL-R cells incorporated with reticulocyte cytoplasts became differentiated 〓 bytalterationcin growth parameters, which might be defined as decancerization:(1) loss of growth ability in soft agar medium;(2) nontumorigenic under heterotransplantation in their filial cellular generation to mude mice;(3) decrease of growth rate, cellular mitoxic index and DNA synthesis rate;(4) decrease or inhibition of c-myc gene expression;(5) expression of human globin gene products in 〓 subculture.

利用C-myc癌基因探针的分子杂交技术，未检测到胞质杂交细胞存在相当于C-myc基因特异的同源序列mRNA，提示杂交细胞癌基因表达严重受抑。

A gene can have many alleles and individuals can have a homozygous or heterozygous genotype.

一个基因可有许多等位基因，而个体可有纯合或杂合的基因型。

Dogs with one mutant and one normal gene are heterozygous, and they are carriers of the condition.

狗带有一个非正常基因和一个正常基因可以分开遗传，也就是说狗们带有着隐性致病基因。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。