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Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升，试验的两种菌株各孔葡萄糖浓度越高，说明葡萄糖消耗越少，经过药物浓度取对数后进行分析，发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系；对氟康唑作用下的两种菌株的线性关系进行分析比较，发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比，其差异基因表达主要集中在：编码蛋白酶体及蛋白水解酶的基因，编码糖脂代谢过程中相关蛋白的基因，细胞周期相关基因，转录及翻译调节相关基因，应激反应相关基因，线粒体相关基因，细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后，其表达变化的基因主要是：编码应激反应家族蛋白的基因，生物膜相关性基因，编码蛋白酶体基因一族，编码细胞周期相关蛋白基因，转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后，其表达变化的基因主要是：编码热休克蛋白基因，丝氨酸/苏氨酸蛋白激酶基因，蛋白酶体家族基因，调控转录及翻译基因。

Then the high speed is needed for separation, and washing can be carried out at the same time, In the process of feeding and high-speed separation, the liquid, under the centrifugal force, is discharged out of the basket through filter cloth and the holes of the basket, and then out of the machine through the tube for discharging liquid, while the solid deposits in the basket. After the separation, open the machine, loosen the lock blocks on lip ring, and suspend the lip ring together with the filter bagt containing cake to discharge.

进料与高速分离过程中，在离心力作用下，液相物穿过滤布及转鼓壁滤孔排出转鼓，经排液管排出机外；固相物截留在转鼓内，分离完毕，停机后启开机盖，松开拦液板锁紧块，将拦液板连同装有滤渣的滤袋一并吊往卸料处卸料。

The implements vertical multilayer light interconnecting with short span, solves upward or decurrent perpendicular catenation of optical path, makes integrated structure.

短跨距实现垂直方向的多层光互连，解决了光路向上或者向下的垂直耦合，制成一体化的结构，层之间的对准误差小，连接损耗低，容易实现软光刻转印，从高聚物转印上能充分发挥软光刻三维转印的优势，易安装；材料选用光学高聚物，具有较低的光学损耗和良好的环境稳定性。

The vibrations can be effectively suppressed under various operation modes, including chopping control and single pulse control, by adopting the novel control strategy. Furthermore, the novel control strategy minimizes the torque ripple under the torque chopping control.

采用新的控制策略，在斩波控制和单脉冲控制下，振动都可得到有效抑止，且新控制策略可在转矩斩波控制下减小转矩脉动。

A drilling apparatus (10) for earth boring operations is of the type having cutter blades (17, 18) mounted on a support body (12, 72, 82) at the lower end of a drill string and includes a novel arrangement of fluid bore sections (15, 23, 23', 63, 64, 65', 92) for delivering fluid under pressure into fluid discharge passages (50, 52) in each of the blades and a bifurcated pivotal mounting portion (16, 74, 91) on the body which enables the bore sections to supply fluid to the cutter blades through a ball joint (22) on the pivotal axis.

一种用于地面钻孔操作的钻井设备(10)，在钻柱的下端具有安装在支撑体(12，72，82)上的切割刀片(17，18)，并包括流体孔部分(15，23，23′，63，64，65，65′，92)和该支撑体上的分叉的枢转安装部分(16，74，91)的新颖结构，该流体孔部分用于在压力作用下将流体供给到每个刀片的流体排放通道中，该安装部分使的该孔部分通过枢转轴线上的球接头(22)能够将流体供给供给到该切割刀片。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Quarrels turns over to quarrel, rides the bicycle equally to be careful, when the bicycle footpath finished, met a hair clip to be curved, my extension furiously, had not thought that I have not turned for the better, straight ran upon has prevented the bicycle to rush to the cliff the Niuzexi guard rail, my luck was luckily good, has not ridden the guard rail, but again I must fall down cliff that class to run upon guard rail's foot.

吵架归吵架，骑单车还是一样要小心，就在单车步道结束时，遇到了一个发夹弯，我奋力的转，没想到我还没转好，就直直的撞上了防止单车冲下悬崖的纽泽西护栏，幸好我运气不错，没有骑过护栏，而是再我要掉下悬崖的那一课撞上了护栏的脚。

Theoretical and experiment researches of transmission mechanism and control strategy of the CVT have been carried out under the project (59835160) financed by National Natural Science Foundation which includes follow content:1The kinematic relation of the metal pushing V-belt type transmission is analyzed. The relation among oil pressure of pulley cylinder, transmission ratio and input torque under load condition is studied. Optimal design method for metal pushing V-belt type continuously variable transmission is put forward.2Based on the engine experiment, torque and oil consumption model of engine are established by method of spline interpolation. The optimum economy and performance curves are put forward.

本文在国家自然科学基金重点项目（资助号：59835160）资助下，对金属带式无级变速传动系统的传动机理、匹配及控制策略等进行了系统深入的理论分析和实验研究，具体研究内容如下：1）系统地分析了金属带无级变速传动的基本运动规律，研究了在负载状态下，带轮油缸压力与速比和输入转矩的关系，提出了对金属带式无级变速传动系统进行优化设计的方法。2）通过发动机台架试验，利用样条插值方法构造了发动机转矩和发动机油耗模型，确定了发动机最佳经济性与最佳动力性工作线。

Especially,in setting of the right turn lane,this paper establishes the critical volume models of setting the right turn vehicle phase and the pedestrians phase in separately,and effective proposes the method of calculating the critical value.

尤其是针对设置了右转专用车道情况下，通过设计右转车辆与行人相位分开设置情况下的流量临界模型，提出了设置二者的相位分开设置的临界值的计算方法。

Western blot analysis with the antiserum ATP95 revealed a significant increase of protein amounts of the V-H〓-ATPase subunits B and c of S. slasa under 100 and 400 mmol/L NaCl treatment, which gave another evidence for a salt-induced coordinate up-regulation of V-H〓-ATPase subunits at transcription and translation levels. The coordinated salt-induced increase of subunit B, H and c of V-H〓-ATPase from S. salsa at transcription and translation levels indicated an increase of V-H〓-ATPase holoenzyme amounts, which might be the reason for the increase of V-H〓-ATPase activity of S. salsa under salt stress.

400 mmol／L NaCl处理盐地碱蓬植株分离其叶片液泡膜微囊进行Western-blot分析发现盐胁迫明显诱导了盐地碱蓬液泡膜H〓-ATPase B、c亚基蛋白的表达，证明盐胁迫下，盐地碱蓬液泡膜H〓-ATPase各亚基在转录、翻译水平存在协同表达。100、400mmol／L NaCl处理亦明显增加了盐地碱蓬叶片液泡膜微囊H〓-ATPase活性，表明盐胁迫下，盐地碱蓬液泡膜H〓-ATPase各亚基在转录、翻译水平的协同表达增加了H〓-ATPase全酶的数量，进而增大了H〓-ATPase的活性。

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。