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In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

For I feel peaceful and comfortable nearside Zhuanjing Lun,without them ,emptiness around me .

没有转经轮我会觉得心空荡荡的，我对转经轮很有信心，只要我有生之年有一口气，我就要弘扬转经轮，只要有客人来，我就赠送转经轮作为礼物，种下善根。

The flux hysteresis controller was ignored in the constant torque region and an improved method was proposed to make the estimation of torque easier.The proper stator voltage space vector was selected based on the output of the torque hysteresis controller and the sector which the current rotor flux locates in.

摘 要：针对无刷直流电机直接转矩控制中磁链观测困难、控制复杂等问题，提出了一种应用于无刷直流电机二二导通方式下的新型直接转矩控制方法：在电机基频以下调速时，略去了常规的磁链观测控制环节，改进了转矩计算方法，通过转矩滞环控制选择所施加的空间电压矢量。

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

Adult rats are divided into control group, intrapericardial injection group and negative group, sublingual vein injection group and negative group. 6ds after transfection, issues of heart, liver, lung and kidney are stained with X-gal to observe transfection to myocardium and non-target transfection. 3. CaN Aβ gene silencing in vivo. Adult rats are divided into Control group, hypertrophy model group, intrapericardial injection group and negative group, sublingual vein injection group and negative group.

二整体动物水平上探讨心肌有效转染途径：以PLacZ为报告基因，将成年大鼠分为空白对照纽(生理盐水心包腔内注射后超声导入)；转染质粒心包腔内注射组及其阴性对照组：质粒+微泡+酶的混合物心包腔内注射，超声导入；转染质粒舌下静脉注射组及其阴性对照组：质粒+微泡混合液经舌下静脉注射，超声导入。

The steady-state temperature distribution in the rolling tire is predicted with finite element method according to the uncoupling idea.

进行了转鼓试验下滚动轮胎稳态温度场的测试；采用试验与计算相结合的方法验证了轮胎在转鼓试验中的实际滚动状态为自由滚动状态；根据解耦的分析思想，进行了转鼓试验下滚动轮胎稳态温度场的有限元分析。

The main motor drive at full speed rotating drum, material feed tube in a row by the introduction of uniform distribution of Drum to the inside wall filter above, in the role of the centrifugal force, the liquid through the filter and drum-wall drum filter exhaust hole, Case liquid discharge pipe machine, solid-phase interception in the material inside the drum to form a circular cake layer.

主电机带动转鼓全速旋转，物料由进料管连续引入，均匀分布到转鼓内段滤网壁上，在离心力作用下，液相物穿过滤网和转鼓壁滤孔排出转鼓，经机壳排液管排出机外，固相物截留在转鼓内，形成环形滤饼层。

Main specifications Model HY800-N HY1200-N The principle product mix The main motor drive at full speed rotating drum, material feed tube in a row by the introduction of uniform distribution of Drum to the inside wall filter above, in the role of the centrifugal force, the liquid through the filterdrum-wall drum filter exhaust hole, Case liquid discharge pipe machine, solid-phase interception in the material inside the drum to form a circular cake layer.

主要产品型号规格 HY800-N HY1200-N 产品结构原理主电机带动转鼓全速旋转，物料由进料管连续引入，均匀分布到转鼓内段滤网壁上，在离心力作用下，液相物穿过滤网和转鼓壁滤孔排出转鼓，经机壳排液管排出机外，固相物截留在转鼓内，形成环形滤饼层。

Consequently,in this study we introduced genes that encode the xylose isomerase and xylulokinase precisely under the control of a strong,constitutive glyceraldehydes-3-phosphate promoter by PCR-mediated overlap extension,which is in charge of xylose assimilation;and also we introduced genes that encode the transaldolase and transketolase precisely under the control of enolase promoter,which is in charge of xylose utilization,then transformed plasmid with the two operons into Z.

因此，本文通过代谢工程手段，将负责木糖代谢转化的木糖异构酶基因和木酮糖激酶基因置于强组成型启动子3-P-甘油醛下；同时将负责木糖转化利用的转醛醇酶和转酮醇酶基因置于强组成型启动子烯醇酶下，通过电转的方式将上述四个基因转入到Zymomonasmobilis CP4中，从而在运动发酵单胞菌中构建起一套完整的木糖代谢途径。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。