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Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

ABSTRACT Aiming at problems of trans-vector controller in a vehicle-carried induction motor such as variable rotor and stator resistance following motor temperature, and their inductance varying with flux saturation degrees. Firstly, CSI and VSI are introduced, for CSI, it can hold the advantages both FOC and DTC using stator flux directional, PI parameter and simulation are also introduced. Secondly, equations of IM in the synchronous rotary frame and its static counterpart, as figuring in the saturation condition of magnetic field, are dealt in this paper and the simulation results are presented. Thereafter, the parameter designing of PI trans-vector controller is processed, which features the non-linear model. In practical realization, as neglecting the dynamical change of motor inductance, this paper employs online modification of inductance parameters, according to the flux saturation, then transforms the non-linear equations into linear ones, and so compacts the structure of controller. Thirdly, in view of the maximum torque output is extraordinary requisite when Electrical Vehicle start up, accelerate, and overtake, this paper issues the maximum torque when both inverter and battery capacity is corporeally definite. Fourthly, on-line differentiating and analyzing both rotor's time constants and its flux by using the expanding order reduction and discrete Kalman filter equation are fathomed, in order to realize high-performance trans-vector algorithm, and also the simulation results are presented. Fifthly, the close-loop regenerative brake system, when the EV's torque is set constant, is deduced; at the same time, unification of electric drive model and brake model is executed. Combined with maximum charge current demands, the mathematical model for online yielding torque as a demand.

本论文针对车载异步电机在矢量控制器所遇到的问题：定转子电阻随温度变化及随电机饱和程度变化的转子电感及定子电感而影响电机调速性能提出一套完整的解决方案：分析了电流型逆变器和电压型逆变器在实现矢量控制时控制器参数的计算，分析出对于电流型逆变器在采用定子磁场定向时，其性能同时具有矢量控制和直接转矩控制两者的优势，并对电压型逆变器在转子磁场定向下的模型进行了仿真研究；建立异步电机非线性模型，推导出考虑磁饱和时异步电机在同步旋转坐标系及静止坐标系下的方程，并做出了仿真结果，建立异步电机在非线性模型下的矢量控制调节器的PI参数设计，在实际应用中，若忽略电机电感的动态变化，可以根据当前的磁路饱和状态而在线修正电感参数，从而将非线性方程线性化，降低控制器的复杂度；考虑到电动汽车在起动和加速超车时需要电机有最大转矩输出，本文讨论在逆变器容量一定和电池供电能力有限的情况下电机最大转矩输出问题；推导了异步电机扩展降阶、离散卡尔曼滤波方程在线辨识转子时间常数和转子磁通，用于实现高性能的矢量控制算法，并给出了仿真结果；推导出了电动汽车恒转矩给定的闭环回馈制动系统，实现了电动控制模型和制动模型的统一，而且结合铅酸电池最大充电电流的要求，为制动转矩在线给定建立了数学模型；设计了基于双DSP系统的高性能矢量控制器软硬件框图，并以大量实验数据说明矢量控制在电动汽车应用的实际应用状况。

Now, I agree that you have to — yeah, there are certain concessions and, you know, if you use a slide projector, you're not able to have the bad type swing in from the back or the side, or up or down, but maybe that's an OK trade-off, to trade that off for a focus.

我现在同意你需要有某种程度的让步，你亦知道如果你使用投映机你将会不能使用那些不好的模式从后边，或从旁边，或从上或下转出来。这或许是一个不错的交换。

Ovary inferior, 1-8-loculed, with 1 pendulous, apotropous ovule per locule.

子房下位，1-8室，每室有1颗倒生的下转胚珠。

Ovary superior, 2-loculed; ovules 2 per locule; placentation axile, appearing nearly basal, apotropous or anatropous, bitegmic, crassinucellar; style simple, connate; stigma discoid or capitate, rarely 4-lobed, not papillate.

子房上位，2室；每个子房室内2胚珠；中轴胎座，近基部着生，下转或者倒生，具双珠被，具厚珠心；花柱单一，合生；柱头盘状或者头状，很少4裂（Tetrastigma，不具乳突。

Compared to WT, the sense-transgenic lines could maintain higher activities of chloroplastic SOD and APX, lower content of chloroplastic O2(superscript -) and H2O2 under salt stress.

与WT相比，盐胁迫下转正义基因植株维持较高的叶绿体超氧化物歧化酶和抗坏血酸过氧化物酶活性，O2和H2O2的含量较低。

Biodiesel is a renewable fuel consisting of the alkyl monoesters of fatty acids which are products of the transesterification of vegetable oils or animals fats with a simple monohydric alcohol in the presence of a suitable catalyst.

生物柴油是一种可再生能源，它是由植物油或动物脂肪与短链醇类在适当的催化剂作用下转酯化反应生成的脂肪酸单酯组成的。生物柴油作为一种可替代、无毒、生物降解性好和可再生性燃料，越来越受到人们的重视。

Malolactic fermentation is a very important processing technology for deacidificaton in modern winemaking.

苹果酸乳酸发酵是现代葡萄酒酿造工艺中重要的生物降酸手段。MLF是L 苹果酸在乳酸菌的苹果酸乳酸酶催化下转变成L 乳酸的酶促反应过程，该过程没有底物水平的磷酸化，但在MLF过程中苹果酸确实能够刺激细菌的比生长速率，增加细菌生物量。

The sea keeping ability has been analyzed on turret single buoy mooring floating production storage unit by short crested wave theory and spectral analysis method for 100 years return period extreme environment in the South China Sea.

用短峰波理论并采用谱分析法，对在南海100年重现期极端环境条件下转塔式单点系泊的浮式生产储油装置的耐波性进行了分析。

Northern blotting analysis indicated that 5 genes, nitrate transporter gene, nitrite reductase gene, ammonium transporter gene, ATP-binding cassette transporter gene, and purine permease gene, were significantly up-regulated under nitrogen starvation.

Northern blotting验证其中5个基因，包括硝酸盐转运蛋白基因、铁氧化还原蛋白亚硝酸还原酶基因、铵盐转运蛋白基因、结合ATP盒的转运蛋白基因和嘌呤透过酶基因，在缺氮诱导条件下转录水平有明显上调。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。