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Six cadavers were prepared for necroscopy, operative procedure of ILIF were simulated on L3-S1, and all related important anatomic structure and their relationship were observed and record. ILIF with transpedicle screw fixation were also performed with cage insertion.3. Twelve fresh frozen bovine lumbar functional spinal units were prepared for biomechanical testing. According to the different treatment order, the specimens were devided into 7 groups, which included: 1intact specimens, 2specimens were treated by left unilateral facetectomy and had homolateral anatomical threaded cages inserted, 3 TLIF with homolateral pedicle screw fixation, 4 TLIF with bilateral pedicle screw fixation, 5 specimens had anatomical threaded cages inserted on the left by intertransverse process approach, 6 ILIF with homolateral pedicle screw fixation, 7 ILIF with bilateral pedicle screw fixation.

方法1、对20名正常志愿者L3-S1节段进行CT增强+三维重建检查，在三维重建图形上测量横突长度、上下横突间距、椎间孔外椎间隙高度及斜径，观查横突间区域血管走形特点并测量血管内径，然后进行统计分析及评价。2、采用福尔马林固定尸体标本六具，模拟ILIF手术对L3-S1节段进行操作，观察横突间区域重要结构的位置和毗邻关系，并模拟同侧椎弓根钉和单枚椎间融合器的置入过程，探讨该术式存在的问题并改进。3、采用小牛脊柱运动节段标本12具，依序进行不同处理后分为以下7组：⑴正常对照组CG⑵左侧小关节切除+椎间融合器植入组(TLIF⑶TLIF附加同侧椎弓根钉固定组；⑷TLIF附加双侧椎弓根钉固定组；⑸左侧横突间入路椎间融合器植入组ILIF⑹ILIF附加同侧椎弓根钉固定组；⑺ILIF附加双侧椎弓根钉固定组。

Species-pairs were positive correlation,including 10 species-pairs were higher significant positive correlation and 24 speciespairs were significant positive correlation, and 263 species-pairs were negative correlation, including 8 species-pairs were higher significant negative correlation and 28 species-pairs were significant negative correlation, which were tested by Spearman＇ s rank correlation coefficient.

正负相关比为0.7643，显著种对占总对数的15.05%，群落优势种种间多呈不显著关联。2）按照31个建群种和优势种对环境的适应方式和主导生态因素，它们被划分为2个生态种组：芦苇组和早熟禾组。3）关帝山撂荒地植物群落以退耕还林的先锋物种为主，表明植物群落处于森林植被恢复的初期演替阶段。

A prediction model has been proposed to deal with threshold voltage shift as a function of 1MeV neutron flux and gate oxide thickness, and to deal with room-temperature annealing of threshold voltage shift induced-by 〓Coγ as a function of electric field and gate oxide thickness. The commonness and individuality of MOS device degradation between hot-carrier effect and ionizing radiation were investigated. The dependence of substrate current, gate current and threshold voltage shift due to hot-carrier on gate oxide thickness were simulated with MEDICI-2D simulator. The photocurrents of PN junction and bipolar transistor were studied. Their transient responses on varied bias voltages, pulse durations and absorbed doses were simulated. The influences of NPN base width on base and collector photocurrents were investigated. We also studied 1MeV neutron displacement damage in PN junction, and calculated reverse current leakage under the neutron flux of 2. 67×10〓 per square centimeter. 3 The study of nondestructive screening method for MOS radiation tolerance A theoretical and experimental study of nondestructive screening methods for radiation tolerance of nMOS and pMOS were firstly fulfilled. We determined the informative parameters pertinent to the method and proposed how to deal with experimental data and verify obtained results statistically, as well as make the required steps for nondestructive screening. Based on the experimental data of 180 samples of discrete pMOS devices, the relation between sample quantity and correlation coefficient, screening equation, and other significant results were obtained from the threshold voltage shifts of pre-and post-irradiation samples.

二、分立器件电离和位移辐射损伤响应研究在国内首次开展了电离辐射引起的氧化层阈值电压漂移和退火理论研究，编程计算了1MeV中子引起的MOS器件阈值电压漂移与中子通量及栅氧厚度的关系，计算了〓Coγ光子引起的阈值电压漂移及退火的时效曲线与纵向电场和栅氧厚度的关系等，对评估MOS型集成电路电离损伤和加固方法研究，以及论文中开展的MOSFETs无损筛选方法研究奠定了基础；分析了MOS器件热载流子损伤效应与辐射电离损伤效应之间的异同性，利用二维模拟软件MEDICI-2D模拟了衬底电流和栅电流，在不同栅氧厚度下随栅压的变化曲线，计算了热载流子效应造成的阈值电压漂移，研究结果对当前存在的以热载流子效应研究取代电离辐射损伤实验研究的设想，具有重要的参考价值；开展了PN结和双极晶体管电离光电流研究，计算了不同偏压、不同辐射脉宽和不同吸收剂量下PN结光电流的瞬时响应，计算了不同辐射脉宽下NPN晶体管基极电流和收集极电流的瞬时响应，以及基区宽度对NPN晶体管光电流的影响，研究结果对双极器件抗电离辐射损伤响应及加固方法研究具有重要意义；在国内首次开展了器件位移损伤的理论研究，分析了辐射产生的缺陷对电性能的影响，计算了注量为2.67×10〓cm〓的1MeV中子产生的缺陷对PN结反向电流的影响。

The concentrations of CO2, CF4, thionyl sulfide (SOF2), sulfuryl fluoride (SO2F2), disulfuryl decafluoride (S2OF10), sulfur dioxide (SO2) and sulfureted hydrogen (H2S) were in partial normal distribution. The concentrations of CO2, CF4, SOF2, SO2F2, S2OF10 were all below 0.1%; the concentrations of SO2 were below 3μL/L; the concentrations of H2S were below 1μL/L. The SF6 byproducts concentrations in 500kV GIS were lower than those in 220kV GIS. The SF6 byproducts concentrations were lower in GIS that have been operating longer. The SF6 byproducts concentrations were significantly different in equipment from different manufacturers.

结果显 示：SF6 GIS 中CO2、CF4、氟化亚硫酰（SOF2）、氟化硫酰（SO2F2）、十氟化二硫酰（S2OF10）、二氧化硫（SO2）和硫化氢（H2S）都成偏正态分布，其中，CO2、CF4、SOF2、 SO2F2、S2OF10 的含量均小于0.1％，SO2 的含量均低于3μL/L，H2S 的含量均低于1μL/L.500kV GIS 设备中SF6 分解产物含量低于220kV GIS 设备；运行时间越长，设备中SF6 分解产物的含量越高；不同设备厂商的GIS 中 SF6 分解产物含量有显著性区别。

The geology research of carbonate reservoirs in Ordovician of Tahe oilfield showed that many large dissolved vugs and fractures were developed. The spread of the reservoir take on a very heterogeneous state. And the typical fractured-vuggy reservoirs was formed. According to the combination of the various spaces, the formation was divided into vuggy formation, fractured-vuggy formation and fractured formation. The research showed that development of the vug is relevant to the its position on the structure, the fractures, the water surface and the weathering surface. Multi-phase charge and recharge of the oil in the reservoirs resulted in the complexity of the fluid distribution. So the"Bottle Model"was brought forward to explain the movement of the water-oil contact. The special storage space and the complex fluid distribution lead to the unstability of the production and the complexity of the water cut. In order to explain the characteristic of the fracture-vug unit was brought forward and the basic principle and method of the partition of the fracture-vug unit was formed. The classification and evaluation of the units were performed according the reserve and energy. Based on the research of geology characteristic and the fluid flow in the reservoir the comprehensive numerical simulation plan of the fractured-vuggy reservoirs were established. Through the selection of the simulation unit, fluid flow type and parameter equivalent the triple media reservoir simulation model was established. Considering the practical application the model was resolved by the DKR decomposition conjugate grads method. Based on the fine reservoir description of Dsitrict IV of the Tahe oilfield the various space type were classified and combined together. The geology model of Unit S48 was constructed. Two typical single well model was established according to the geology and the dynamic phenomena. Finally the single well model and the Unit S48 were simulated by the triple media reservoir simulator. Via the local grid refinement and coarsening in the simulation good matchs were gained. Based on the results of the simulation the reserve distribution, percent of reserve produced in various space and the natural energy were analyzed. The fluid was storaged in the fractures and vugs mostly and more than 90% the produced oil came from the systems. The energy belonged to the active one. The results of simulation accorded with the fact and showed the validity and practicability the research and the simulator.

塔河油田奥陶系碳酸盐岩油藏的地质研究表明，其储层中发育着很多大型的溶蚀洞、缝，储层的平面展布呈现出极度的非均质性，形成了典型的缝洞型碳酸盐岩油藏，根据各种孔隙介质在储层中的组合，将储层分为了溶洞型、裂缝-溶洞型和裂缝型三类；研究表明溶洞储层的发育和构造位置、裂缝的发育、潜水面和风化面的位置等因素有关；多期充注的油气成藏模式导致了流体复杂的赋存状态，由此提出了所谓的"瓶子模型"，解释生产过程中油水界面的变化；特殊的储集空间类型和流体分布特征导致油田在开发过程中表现出很大的不确定性和含水变化的复杂性，为了合理的解释油气田开发过程中的动态特征，提出了"缝洞单元"的概念，并制定了"缝洞单元"纵横向划分的基本原则和依据及划分方法，并对"缝洞单元"进行了分类和评价；基于地质特征和流体在其中流动规律的研究，提出了缝洞型碳酸盐岩油藏的数值模拟综合解决方案，通过模拟单元的选择、流动类型和参数的等效，建立了三重介质油藏三维三相数值模拟模型，采用不完全LU分解预处理共轭梯度法进行了求解；在塔河油田4区精细油藏描述的基础上，将各种类型的孔隙空间进行了归类组合，建立了S48单元的地质模型；通过对油井生产动态进行分析研究，建立了两类和油井地质、生产动态相对应的单井模型；最后应用三重介质油藏数值模拟软件对单井模型和S48单元进行了数值模拟，通过局部加密和粗化等技术模拟流体流动规律，取得了很好的拟合效果；结合数值模拟结果，分析了各种介质中的储量分布、储量产出的百分比以及地层的能量，认为塔河油田缝洞型油藏中流体绝大多数储集于缝洞系统之中，所产出流体90%以上也来自于缝洞系统，其底水能量属于较充足的类型；模拟结果和油田实际情况符合较好，说明了地质研究和油藏数值模拟研究的正确性。

The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

When the aphids were feeding on Ww2730 seedlings, the time to 1st probing was delayed compared to those on Xiaoyan22 and Batis, searching was interrupted before 1st probing, and 1st probing was shorter than that on Xiaoyan22 and Batis significantly. Duration of pd Ⅱ-1 on Ww2730 and Xiaoyan22 was much longer than that on Batis. Duration of pd in C waveforms on Ww2730 was longer than that on Xiaoyan22 and Batis remarkedly. Times and duration of G waveform on Ww2730 were more and longer. Times of spot G waveform on Batis were more than on Ww2730 significantly, but the duration was shorter than those on Xiaoyan22 and Ww2730. Total time of E1 waveform, max longest duration of E1 fractions and mean duration time of E1 fractions following E2 wavefrom on Xiaoyan22 and Batis were all longer than on Ww2730 statistically. There were no differenences among mean durations of 1st E1 wavefrom on three varieties, but mean durations of other E1 waveforms on Ww2730 and Xiaoyan22 were shorter than those on Batis. The other waveform parameters, include times and durations of F and E2 were all not different on three wheat varieties.It is suggested that the resistance mechanism of wheat variety Ww2730 to S.

麦长管蚜在品种Ww2730上口针开始接触叶面时间显著晚于小偃22和Batis，第1次持续刺探前的刺探次数显著多于小偃22和Batis，开始刺入叶面后第1次刺探的持续时间极显著短于小偃22和Batis；在Ww2730和小偃22上的PdⅡ-1的平均时间显著长于Batis；Ww2730上C波阶段2个Pd波间的间隔平均时间显著大于另外2个品种；在Ww2730木质部取食的次数和时间显著大于其它2个品种；Batis上的点G波的次数极显著地高于Ww2730，而平均持续时间极显著短于小偃22和Ww2730；在Batis和小偃22上E1波的总时间，E1波的最大值、跟随E2波的E1波的平均时间均显著大于Ww2730；3个品种间第1次E1波持续的时间差异不显著，但Ww2730和小偃22上其它E1波持续的时间极显著少于Batis；3个品种上其它波形参数，包括F波的次数和总时间、E2波的次数和总时间没有差异。

At 4 weeks following implantation, cells on the surface of coralline hydroxyapatite were found and connective tissues were seen in the material pores in the experimental group. Cells on the coralline hydroxyapatite were observed only in the control group. At 8 weeks, new bone formation was detected on the surface of coralline hydroxyapatite; bony tissue deposition and a few chondroid tissues were found in the pores or surrounding the pores in the experimental group. A few fibrous connective tissues were observed in the control group. At 12 weeks, abundant mature woven bone was detected on the surface of coralline hydroxyapatite; medullary cavity-like structure and vessels were found in some regions in the experimental group. No new bone or bony tissues were found in the control group.

植入材料后4周，实验组可见珊瑚羟基磷灰石表面有细胞生长，孔隙内有结缔组织长入；对照组仅见珊瑚羟基磷灰石表面有细胞生长。8周时珊瑚羟基磷灰石表面有新生骨形成，孔隙内和孔隙边缘可见骨样组织沉积和少量软骨样组织形成；对照组仅见少量纤维结缔组织长入。12周时珊瑚羟基磷灰石材料表面有较多成熟编织骨形成，部分区域可见髓腔样结构形成，并有血管长入；对照组仍未见新骨及骨样组织形成。

The results showed that the D50 of Astragalus membranaceus and Epimedium brevicornum ultra-fine powder were 6.82μm and 8.40μm separately. The broken cellular walls of two ultra-fine herbal powder were bigger than those of the commonly cribble, and intact cells were not visible under SEM, and it improved the mixed uniformity. On the contrary, organization blocks of the commonly cribble were clearly discernible, also the shape and granularity were irregular, and the organization cells were still complete. The bulk density and liquidity of Astragalus membranaceus and Epimedium brevicornum increased significantly after ultra-fine Smashing. The component icariin extraction content of the ultra-fine powder was enhanced by 18.31% compared with the cribble by the chromatograph analysis of icariin. At the same time, Astragalus membranaceus polysaccharide and Epimedium brevicornum polysaccharide content of the ultra-fine powder were enhanced by 117.13% and 315.15% compared with the cribble.

结果发现黄芪和淫羊藿经超微粉碎后，粉体的粒度分布中心D_(50)分别为6.82μm和8.40μm，经电镜观察均难以看到完整的细胞形态，只能看到细胞碎片，破壁率远远大于普通粗粉，其混合的均匀度显著提高，而普通粗粉在显微镜下，组织块清晰可见，且形状不规则，粒径分布不均匀，组织细胞仍然完整；超微粉碎后，黄芪和淫羊藿的堆密度和流动性显著增强；通过对淫羊藿有效成分淫羊藿苷的色谱分析，其中淫羊藿超微粉的淫羊藿苷体外溶出量较淫羊藿粗粉中提高了18.31%；同时黄芪、淫羊藿超微粉多糖的提取率分别较黄芪、淫羊藿粗粉提高了117.13%，315.15%。

Means the parent directory, so this command means to execute "toolchain.sh," which is in the current directory.

代表父目录，所以这个命令就是执行当前目录下的"toolchain.sh"。

Yes,In fact,I'm on our city ream.

是的。事实上我是我们市队的。