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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证明。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy（32/120; 26.7%）. The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders（22/120; 18.3%）. Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found（12/120; 10%）. Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters（8.3%）. Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters（4.2%）had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现，在总共搜集的120个病例中，其中因年老所导致的产精力不佳为最多，占38例（31.7%），於镜下可见大量精虫黏附於Sertoli cell的表面，并可见Sertoli cell数量明显增多而Leydig cell明显减少，且其细胞核呈现浓染的现象，而在其副睪中仍可见到成熟精虫蓄留於管腔中，但在其输精管内却只有少量精虫存在；其次为睪丸萎缩，占32例（26.7%），镜下可见中度至重度无造精作用，其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在，但Leydig cell数量明显减少且其细胞质内的脂质也明显减少；睪丸发育不全，占22例（18.3%），於生精小管内只见到精母细胞及少量Sertoli cell存在，不见造精细胞分化，於生精小管间质可见Leydig cell减少并伴随结缔组织增生；副睪结石，占12例（10%），肉眼下可在副睪及输精管前段见到黄白色大小不一的结节，触感坚硬，於镜下可见副睪管扩张并有大量成熟精虫蓄积，严重时可见钙化现象，以Von Kossa及茜素红染色均呈阳性反应；类淀粉沉著症，占10例（8.3%），镜下在睪丸间质及血管周围可见粉红均质样的物质沉积，以刚果红染色成阳性反应；马立克病，占5例（4.2%），镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润；睪丸坏死，占1例（0.8%），镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

The results showed: when TLC solvent system was: chloroform - methanol - diethyl amine = 90:9:1, alkaloids from lotus leaves were well isolated, and five components were isolated and identified by spraying Dragendorff reagent. The optimal conditions of HPLC solvent system were: methanol- water - diethyl amine = 75:25:0.0125. Under the conditions, components of alkaloids from lotus leaves were separated very well, and ideal RP-HPLC peaks were obtained.5、Methods of High Speed Counter Current Chromatography and preparative High Performance Liquid Chromatography applied to identify and purify alkaloids from lotus leaves were set up. When solvent system of HSCCC was: chloroform - methanol - water (pH=4.00)= 4:3:2, speed of chromatogram was 700rpm, flow speed of the mobile phase was 2mL/min, four pure components relatively were attained by isolation and purification.

结果表明：薄层层析溶剂系统为氯仿：甲醇：二乙胺=90：9：1，使荷叶中的生物碱达到了较好的分离效果，并用改良的碘化铋钾试剂喷雾显色，共分离鉴别出了5种荷叶生物碱；分析型HPLC分析检测的较优溶剂系统为甲醇：水：二乙胺=75：25：0.0125，使荷叶生物碱的各个组分达到了基线分离，并获得了较好的峰形。5、建立了高速逆流色谱和制备型HPLC技术分离纯化荷叶生物碱的具体方法。H(来源：ABC46论文网www.abclunwen.com)SCCC法在溶剂系统为氯仿：甲醇：pH4.00的水=4：3：2，色谱仪转速为700rpm，流动相流速为2mL／min的条件下，分离纯化得到了4个纯度较高的化合物。

Results: The prevalence rates within two weeks among the elderly were 29.7% and 64.7%, and the rates of seeing a doctor within two weeks were 9.5% and 34.7% in urban community and rural areas respectively. The prevalence rates of chronic diseases were 62.1% and 56.4% in city and rural area respectively. Sixty-six point nine percent of the elderly in urban community went to high-level hospitals for medical services, while 73.3% of old people in rural area went to village clinics. The main reasons for diseased people not to visit a doctor were self feeling of slight illness (21.7%) and self-treatment (51.6%) in urban community and were low income (40.8%) and self feeling of slight illness (25.9%) in rural areas. The main affecting factors to rates of seeing a doctor within two weeks were increased age and family income per capita among the elderly in urban community, and were suffering or not suffering from chronic diseases in rural area.

结果：城市和农村社区老年人的两周患病率分别为29.7%和64.7%，两周就诊率为9.5%和34.7%，慢性病患病率分别为62.1%和56.4%；城市老年人就诊地点主要以县市级和省级医院为主，两者所占比例为66.9%；农村则以个体诊所或村卫生室为主，占73.3%；两周患者未就诊原因在城市主要是自感病轻（占21.7%）和自我医疗（占51.6%），在农村主要为经济困难（占40.8%）和自感病轻（占25.9%）；影响老年人两周就诊的因素在城市为年龄和家庭人均月收入，在农村是患慢性病与否。

The F-P structure, especially the thermal-strain mechanism of thermal bimetal sheets were investigated deeply in the paper, the Finite Element Analysis results for both the "four nodes" and the "ring" confinements were given; the size of the ring hole can be simply changed to agilely meet the requirement of the different measurement range; the materials match and the parameters selection of the thermal bimetal sheets were analyzed and discussed, and some available suggestions were given; the F-P temperature sensors with the two developed bimetal sheets confinement mechanism were designed and fabricated, the practical measurements were carried out, and the experiment results were analyzed and discussed.

文中对所提出的F-P腔结构，特别是热双金属片的应变机制进行了较系统深入的研究，给出四点约束和环状约束具体有限元分析结果；提出的环状约束可以简单改变环孔大小灵活适应不同量程的需要；对热双金属片材料配对和参数选择进行了分析讨论，并给出有益的建议；实际制作了两种约束机制的光纤热双金属片F-P腔温度传感器，进行了相关实际测试，并对实验结果进行了讨论。

If, in the absence of these movements, the landlords, rich peasants, counterrevolutionaries, bad elements and monsters were all allowed to crawl out, while our cadres were to shut their eyes to all this and in many cases fail even to differentiate between the enemy and ourselves but were to collaborate with the enemy and were corrupted, divided and demoralized by him, if our cadres were thus pulled out or the enemy were able to sneak in, and if many of our workers, peasants, and intellectuals were left defenseless against both the soft and the hard tactics of the enemy, then it would not take long, perhaps only several years or a decade, or several decades at most, before a counterrevolutionary restoration on a national scale inevitably occurred, the Marxist-Leninist party would undoubtedly become a revisionist party or a fascist party, and the whole of China would change its color.

不然的话，让地、富、反、坏、牛鬼蛇神一齐跑了出来，而我们的干部则不闻不问，有许多人甚至敌我不分，互相勾结，被敌人腐蚀侵袭，分化瓦解，拉出去，打进来，许多工人、农民和知识分子也被敌人软硬兼施，照此办理，那就不要很多时间，少则几年、十几年，多则几十年，就不可避免地要出现全国性的反革命复辟，马列主义的党就一定会变成修正主义的党，变成法西斯党，整个中国就要改变颜色了。

If, in the absence of these movements, the landlords, rich peasants, counterrevolutionaries, bad elements and monsters were all allowed to crawl out, while our cadres were to shut their eyes to all this and in many cases fail even to differentiate between the enemy and ourselves but were to collaborate with the enemy and were corrupted, divided and demoralized by him, if our cadres were thus pulled out or the enemy were able to sneak in, and if many of our workers, peasants, and intellectuals were left defenseless against both the soft and the hard tactics of the enemy, then it would not take long, perhaps only several years or a decade, or several decades at most, before a counterrevolutionary restoration on a national scale inevitably occurred, the Marxist-Leninist party would undoubtedly become a revisionist party or a fascist party, and the whole of China would change its color.

阶级斗争、生产斗争和科学实验，是建设社会主义强大国家的三项伟大革命运动，是使共产党人免除官僚主义、避免修正主义和教条主义，永远立于不败之地的确实保证，是使无产阶级能够和广大劳动群众联合起来，实行民主专政的可靠保证。

Methods ①The spiral organ of corti of 10 mice (20 ears) were taken, and divided into 2 groups randomly (n=10), cultured in 0.1 mol·L-1 gentamicin and the metabolite of 0.1 mol·L-1 gentamicin for 48h, then the number of hair cells remain was recorded;②The spiral organ of corti of 10 mice were taken (20 ears), and divided into 2 groups randomly (n=10), 3% CaCl2 and 0.1 mol·L-1 GM were mixed in the culture medium and the number of hair cells remain in the two medium mentioned above were recorded;③The spiral organ of corti of 10 mice were taken (20 ears), divided into 2 groups randomly (n=10), 3% MgSO4 to 0.1 mol·L-1 GM were mixed in the culture medium, the number of hair cells remain in the two medium mentioned above were recorded.

①取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM代谢产物的培养液中培养48 h后，记数残留的耳蜗毛细胞数；②取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM+3% CaCl2的培养液中培养48 h后，记数残留的耳蜗毛细胞数；③取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM+3% MgSO4的培养液中培养48 h后，记数残留的耳蜗毛细胞数。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。