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Results About 39 cases were diagnosed as benign tumors,majority of which were demoid cysts.The another one case was borderline mucinous cystadenoma.Eight cases were diagnosed by ultrasound examination during the first trimester(53.33 %),4 had complication to do emergency operations,3 were caused by torsion,one by rupure.Serum samples for CA125 estimation were obtained from 3 before operations.AS a result,2 cases were elevated,one of which was borderline mucinoers cystadenoma.38 cases were full-term birth.

结果 妊娠期良性肿瘤39例，占97.5%，以生殖细胞肿瘤多见，黏液性囊腺瘤交界型1例；孕早期超声诊断8例，占孕期发现总例数的53.33%(8/15)；有4例出现并发症并行急诊手术，扭转3例，破裂1例，术前3例行CA125检查，2例升高，其中1例为黏液性囊腺瘤交界型；足月分娩38例，占95.00%。

Results Of 10 cases with internal impingements,MR showed infraspinatus abnormalities were 8 cases.Supraspinatus abnormalities were 4 cases.Labral abnormalities were 9 cases.Posterosuperior humeral head damage were 10 cases.10 cases with subacromial impingement.MR showed infraspinatus abnormalities were 2 cases.Supraspinatus abnormalities were 8 cases.Labral abnormalities were 0 case.

结果 10例内在撞击综合征MR表现为冈下肌腱异常者8例，冈上肌腱异常者4例，盂唇异常者9例，肱骨头后上部异常10例。10例肩峰下撞击综合征MR表现为冈下肌腱异常者2例，冈上肌腱异常者8例，盂唇异常者0例，肱骨大结节异常8例。

Results MRI appearances of acoustic neuromas were:（1）The turmor's growth round the internal auditory canal,the Ⅶ,Ⅷ nerves affected side were thickened than that of opposite side.There was no marked demarcation between the main bodies of the tumors and the affected nerves.The connection between tumors and affected nerves could be showed at contrast enhanced imaging.（2）The tumors were of hypo or isohypo intensity on T1 weighted images and hyper or iso hyper intensity on T2 weighted images.（3）After Gd DTPA administration the tumors were homogeneously or inhomogeneously or circularly enhanced.（4）The margins of the tumors were smooth and well delineated.（5）The affected internal auditory canal usually became shorter in depth and larger in posterior anterior diameter.（6）MRI appearances of micro acoustic neuromas were that the affected Ⅶ,Ⅷ nerves were normal in size or nodularly thickened and the tumor reveaed nodular or point enhancement at contrast enhanced MRI.

结果 听神经瘤的MRI表现主要为：（1）肿瘤以内听道为中心生长，第Ⅶ、Ⅷ神经束较对侧增粗，与肿瘤主体多无明确分界，增强后可见两者相连，颇为特征；（2）肿瘤在T1加权图像上呈低信号、略低信号或低等混合信号，T2加权呈高信号或高等混合信号；（3）注射Gd DTPA后肿瘤呈均匀、不均匀或环状强化；（4）肿瘤边界清晰，边缘光整；（5）绝大多数肿瘤患侧内听道前后径扩大，深径缩短；（6）微小听神经瘤的MRI表现为第Ⅶ、Ⅷ神经束正常或局部结状增粗，造影后呈结节状或点状增强。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

Salix matsudana, Ulmus pumila, Quermus mongolica, Populus alba×P.beroliensis and Betula platyphlla are frontal 5 tree species with weight value 90.37, 90.12, 88.12, 87.55 and 86.2 respectively, were used for farmland protection forest; Acer negundo, Salix matsudana, Juniperus rigida, Fraxinus mandshurica and Ulmus pumila are frontal 5 tree species with weight value 78.14, 76.92, 75.96, 74.72 and 74.56 respectively, were used for urban protection forest; Ulmus pumila, Salix matsudana, Populus alba×P.beroliensis, Acer negundo and Larix gemelinii are frontal 5 arbor tree species with weight value 80.07, 79.07, 77.5, 72.42 and 71.67 respectively, were used for road protection forest; Salix matsudana, Populus alba×P.beroliensis, Ulmus pumila, Larixgemelinii and Betula platyphlla are frontal 5 tree species with weight value 92.37, 88.72, 86.62, 85.82 and 85.7 respectively, were used for river protection forest; Fraxinus mandshurica, Larix gemelinii, Populus alba*P.beroliensis, Ulmus pumila and Salix matsudana are frontal 5 tree species with weight value 89.61, 8.35, 87.12, 85.56 and 80.15 respectively, were used for wood forest; Cornus alba, Prunus tomentosa, Primus triloba, Syringa obalata and Sambucus williamsii are frontal 5 shrubs with weight value 82.93, 81.23, 80.85, 78.38 and 77.78 respectively, were used for landscape forest; and Acer negundo,Malus baccata, Sorbus pohuashanensis, Prunus maackii and Prunus padus are frontal 5 arbors with weight value 82, 81.52, 80.3, 79.62 and 78.9 respectively, were used for landscape forest; Prunus salicina, Prunu

根据哈尔滨地区不同林种的功能要求，给出了不同指标的期望值，通过查询系统可以得到旱柳、榆树、蒙古栎、银中杨、白桦为符合农田防护林的前五位的树种，权重值分别为90.37、90.12、88.12、87.55、86.2；糖槭、旱柳、杜松、水曲柳、榆树为符合城市防护林的前五位的树种，权重值分别为78.14、76.92、75.96、74.72、74.56；榆树、早柳、银中杨、糖槭、兴安落叶松为符合道路防护林的前五位的乔木树种，权重值分别为80.07、79.07、77.5、72.42、71.67；旱柳、银中杨、榆树、兴安落叶松、白桦为符合河岸防护林的前五位的树种，权重值分别为92.37、88.72、86.62、85.82、85.7：水曲柳、兴安落叶松、银中杨、榆树、旱柳为符合用材林的前五位的树种，权重值分别为89.61、88.35、87.12、85.56、80.15；红瑞木、毛樱桃、榆叶梅、紫丁香、接骨木为符合风景林前五位的灌木树种，权重值分别为82.93、81.23、80.85、78.38、77.78，糖槭、山丁子、花楸、山桃稠李、稠李为符合风景林前五位的乔木树种，权重值分别为82、81.52、80.3、79.62、78.9；李、杏、文冠果、山楂、野梨为符合经济林的前五位的树种，权重值分别为88.85、80.88、73.25、69.35、63.77。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

Methods: 81 cases with elderly NSCLC were divided into two groups randomly,43 cases in the treatment group were administrated with Sinoactionomycin 6g/kg (1~4 day) combining card platinum 300mg/m2 (1st day), glucoside (VP16) 100 mg (1~4 or 5days) intravenously.38 cases in the control group were only treated with CBP and vp16 and the dosage were same with that of treatment group.21~28 days were taken as a treatment cycle and the curative effects were studied after 2~3 cycles.The relievable and survival periods were investigated as well.

选取老年NSCLC病例81例，随机分治疗组43例给予新福菌素6μg/kg（第1~4d），卡铂300mg/m2（第1d），依托泊苷（VP16）100 mg（第1~4或5d）静脉滴注；对照组38例单纯使用CBP和VP16，剂量和方法同治疗组，21~28d为一个疗程，2~3个疗程评价疗效，随访缓解期和生存期。

Results (1) From more than 100 primers, 11 primers producing polymorphism and reproduceable bands were selected, 129 bands were amplified.(2) The percentage of polymorphic bands within different populations were 20.9%-55.0%.(3) The cluster map including all samples were obtained by UPGMA. In the map, there were six cluster groups and three individuals outside the groups. The branch with five samples of Zhongjiang Sichuan population were far from other samples in genetic distance.(4) According to the distribution provinces five groups were divided in genetic variance analysis. Genetic variance 80.44% existing within population, 8.29% among populations and 11.27% among groups.

结果 （1）从100多个引物中选择出11个多态性强和重复性好的引物，扩增得到129条带；（2）不同居群内多态位点比率为20.9%－55.0%；（3）用UPGMA法得到所有样本的聚类图，可分为6个主要分支组和3个组外个体，其中四川中江居群的5个样本聚为一组，并与其他样本遗传距离较大；（4）按产地分组，遗传差异的80.44%存在于居群内，8.29%来自于组内居群间，11.27%的遗传差异来自于组间。

Resultantly, the scores of the syndromes as indicated in the cancer group were higher (Qi deficiency syndrome:37.62±16.69 vs 19.92±13.36; blood stasis syndrome:28.14±9.34 vs 12.60±8.80) than those in the non-cancer group. The incidence of the syndromes in the latter group was lower (Qi deficiency syndrome :25.7% vs 67.0%; blood stasis syndrome :21.1% vs 87.0%) than the former group.The scores of the syndromes in the late cancer stage were higher than those in the early stage, but the incidences of the diagnostic patterns for the syndromes were similar. The scores of qi deficiency were higher during chemotherapy than after or without chemotherapy, those of blood stasis were similar during, after or without chemotherapy, those of the syndromes were not different with or without radiation therapy, and those of the syndromes were reduced by the two-week Traditional Chinese medicine therapy.

结果显示癌症组的气虚分数37.62±16.69比非癌症组的19.92±13.36高；癌症组的血瘀分数28.14±9.34也比非癌症组的12.60±8.80高；非癌症组气虚证的发生率25.7%比癌症组的67.0%低；非癌症组血瘀证的发生率21.1%也比癌症组的87.0%低；癌症晚期比早期有较高的气虚和血瘀分数，但证型发生率两者相似；正在化疗中的气虚分数比曾经化疗或无化疗者高，但血瘀分数则三者相似；放疗有或无不会影响癌症患者的气虚或血瘀分数；中医治疗介入两周能减少癌症患者的气虚和血瘀分数。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。