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The analysis methods for the determination of microor trace elements in high moisture jellyfish were developed. The fatty acid compositions indifderent parts of fresh jellyfish were determined by GC/MS method. Thirty-five fatty acids wereidentified, and most of them were found in R. esculentum jellyfish for the first time. Especially,two unusual very long chain polyunsaturated fatty acids that were never detected in the otherjellyfish also were determined. Amino acids were abundant in R. esculentum jellyfish, especiallycontaining sulfur amino acids, and could be supplied for human diet. The polysaccharide inumbrella part of jellyfish was composed of glucose, galactose and uronic acid, and its molecularweight was 40,000, but the polysaccharide of the oral arms part consisted of glucose, mannose andglycuronic acid, and its molecular weight was 43,000. Above-mentioned data were never reported.The ethanolic extract of oral arms part of jellyfish were extracted by different polar solvents(petroleum ether, acetic ether, n-butanol), and antibacterial activity was tested to these extracts byfour species of terricolous pathogenic bacilli and three species of botanic pathogenic fungi. Theresult demonstrated that the petroleum ether extract had certain bactericidal activity for twospecies of pathogenic bacilli, and n-butanol extract had certain inhibited activity on apple rotpathogenic fungus.

建立了高含水量的海蜇产品中微量、痕量元素成分测定的分析方法；采用 GC/MS 方法测定了新鲜海蜇不同部位的脂肪酸组成，共鉴定出 35 种脂肪酸，其中大多数脂肪酸是首次在海蜇中被检测到，尤其是两种不常见的 C24：5 超长链多不饱和脂肪酸的分析和鉴定在其它水母种属中也从未见报道；海蜇三个部位中氨基酸成分齐全，含量丰富，含硫氨基酸含量较高，可与其它食物蛋白质的氨基酸互补；其中海蜇皮多糖是由葡萄糖、半乳糖和糖醛酸组成，分子量为 40，000，海蜇头多糖是由葡萄糖、甘露糖和糖醛酸组成，分子量为 43，000，以上工作均未见报道；利用石油醚、乙酸乙酯、正丁醇三种不同极性溶剂分别萃取海蜇头乙醇浸提物，用纸碟法和生长速率法分别对四株陆源病原菌和三株植物病原真菌进行了抑菌实验，结果表明海蜇头石油醚提取物和正丁醇提取物具有一定的抑菌活性。

Result: Scoliosis was identified in 58 cases with Marfan syndrome (38 males and 20 females), the prevalence rate was 42.03%, male-female sex ratio was 1.18:1, 6 cases were in younger than 10-year-old group. 12 cases were in 11~20-year-old group, 19 cases were in 21~30-year-old group, 11 cases were in 31~40-year-old group, 7 cases were in 41~50-year-old group, 3 cases were in 51~60-year-old group. Mean magnitude of Cobb angle in coronal plane was 26.8°±27.8°, the types of scoliosis curve included thoracic curve (36 cases), thoracolumbar curve (11 cases), lumbar curve (2 cases), double curve (6 cases) and triple curve (3 cases), apex vertebraes were convex to the right side among single curves in 38 cases while 11 cases were convex to the left side. Mean magnitude of kyphosis in sagittal plane was 14.3°±13.2°, 5 patients had thoracic lordosis and 40 patients had hypokyphosis and 12 patients had normal kyphosis.

结果：58例(42.03%)患者合并脊柱侧凸，男38例，女20例，男女患病率比例为1.18：1，其中≤10岁6例，11~20岁12例，21~30岁19例，31~40岁11例，41~50岁7例，51~60岁3例；平均冠状面Cobb角为26.8°±27.8°；胸弯36例，胸腰弯11例，腰弯2例，双弯6例，三弯3例；单弯中顶椎凸向右侧38例，凸向左侧11例；矢状面胸椎后凸平均为14.3°±13.2°，其中胸椎前凸5例，胸椎后凸不足40例，胸椎正常后凸12例，仅1例胸椎后凸45°；11例患者冠状面Cobb角＞40°，平均年龄15.9岁。

Angiectasis, permeability were increased , inflammatory cell were transuded; The cardiac muscle injury conditions after hypoxia 4 h were similar with hypoxia 2h group, appeared lipid droplet, implied the oxidative metabolism of lipid got obstacle started from then; The cardiac muscle injuried conditions were got little improvement in hypoxia 6 and 8 hours team, mitochondria were increased and aggregatived, normal cell junction, sarcomere contract weaked; in hypoxia 10h group , mitochondria were increased and aggregatived obviously, a few glycogenosome were assembled, transverse tubule were expanded, all the changes may be the adaption of Gansu zokors in the hypoxia tolerance process to 4% hypoxia extent gradually.

在4%严重低氧耐受过程中，甘肃鼢鼠心肌超微结构在2h组出现血管扩张，通透性增加，有炎细胞渗出，异染色质边集；4h组心肌损伤状况与2h组接近，出现脂滴，提示脂类的氧化代谢在此时开始发生障碍，6和8h组心肌损伤状况逐渐好转，线粒体聚集增多，细胞连接正常，肌小节收缩较前有所减弱；10组线粒体增多聚集，线粒体脊增粗更为明显，少量糖原颗粒聚集，脂滴较前有所减少，横小管扩张增大变粗，出现了与能量代谢有关的结构适应性变化。

This in vivo study was to examine the historical changes of implanted novel chitosan/collagen composite barrier for confirming the clinical feasibility. Four other commercial GTR membranes were chosen for comparison. Among the resorbable GTR membranes, BioMend Extend and Peri-Aid are collagen base, and GORE-TEX OSSEOQUEST is synthesized membrane, while GORE-TEX e-PTFE (Expanded polytetrafluoroethylene) is synthesized but non-resorbable. Beagle dogs were used as animal model. Buccal mucoperiosteal flaps were reflected in the bilateral mandibular premolar and molar areas. Buccal alveolar bone was reduced on 1st、2nd premolar and molar to a level 5 mm apical to the cemento-enemel junction. Root surface was denuded of periodontal ligament and cementum, and notches were placed at the bone level of each root. The tested GTR barriers were implanted in critical bone defect areas. Flaps were coronally positioned and sutured. Two beagle dogs were sacrificed each time as the designed time period after surgery. Histological and histometirc evaluation at 7 days、14 days、28 days、3 months were performed post-operatively to determine the healing response of each treatment modality.

本研究之目的在评估与工研院生医中心合作发展出可吸收性去乙醯几丁聚醣/胶原蛋白复合之组织导引再生膜片，并选择与可吸收性之BioMend Extend 及Peri-Aid 胶原蛋白膜片，和GORE-TEX OSSEOQUEST 合成高分子膜片，及不可吸收之GORE-TEX e-PTFE合成高分子膜片，等其他四种市售商品材料共同对照评估，应用8只年龄为12个月的雄性小猎犬，均分4组(分别为7天、14天、28天、3个月)，为动物活体评估模式，在实验犬之左、右下颚第一、二小臼齿及大臼齿的颊侧区制造骨缺损后，分别植入组织导引再生膜片，依实验设定时间将小猎犬牺牲，取下缺损区骨头，以光学显微镜观察量测其牙垩质再生高度与齿槽骨再生高度之变化，以探讨其组织再生模式及评估新膜片在临床应用之适用性。

PCR,urethrascope or biopsy was applied to some patients.52cases were seminal vesiculitis,28cases were prostatitis,29cases were genital tuberculosis,26cases were adenoma of the posterior urethra,11cases were cyst of prostate and seminal vesicle,8cases were hemospermia of postintercourse,9cases were idiopathic.They were treated by medicine,operation and adjuvant ways respectively.5cases of epididymal tuberculosis underwent epididymectomy because of deficiency of medicine.2cases of adenoma of the posterior urethra after electrotomy recured,which underwent reoperation.

诊断 精囊炎52例，前列腺炎28例，生殖系统结核29例，后尿道腺瘤26例，前列腺和精囊囊肿11例，性交后血精8例，特发性血精9例，分别给予药物、手术或对症治疗。5例附睾结核药物治疗效果不佳而行附睾切除术，后尿道腺瘤行肿瘤电灼术后复发2例，再手术后短期未见复发。

Methods:Of 70 cases with traumatic pelvic fracture associated with injuries of other organ, 16 were treated by lienectomy, 7 were treated by liver repair, 6 were treated by colon operation, 6 were treated by intestine inosculation, 4 were treated by bladder fistulatome, 10 were treated by ligation of internal iliac arteries, 1 was treated by half-pelvic excision, 4 were treated by angioembolization, 5 were treated by conduction.

目的：探讨外伤性骨盆骨折合并其他部位伤的诊治方法及最佳适应证方法：对71例外伤性骨盆骨折合并其他部位伤的病例进行不同方法的诊治：脾切除16例，肝修补7例，结肠手术6例，小肠修补吻合6例，膀胱造瘘4例，髂内动脉结扎10例，半骨盆离断术1例，髂血管探查取栓4例，胸腔闭式引流5例。

Methods Rabbits were randomized to three groups. Complex model group were fed with feed containing 0.5% cholesterol for 3 weeks. At the fourth, eighth and twelfth weeks, rabbits were given injection of lipopolysaccharide (1 μg) at auricular artery, cervical part and fold inguen, respectively. Normal group were fed with normal diet, while High cholesterol group were fed with feed confaining 0.5% cholesterol only. After 16 weeks, vascular tissues were analyzed using morphometric and histological methods, and the sera were checked for cholesterol, LDL-C, C-reaction protein and tumor necrosis factor-α. Results The cholesterol and LDL-C of High cholesterol group were increased, compared to those of normal group.

给含0.5%胆固醇的饲料，3周后，分别在第4、8、12周采用耳动脉内、颈部、腹股沟处肌肉注射大肠埃希菌脂多糖，并设立正常组和单纯高脂组。16周后观察兔的一般状态，取血清检查血脂六项、C-反应蛋白和TNF-α，取耳动脉、颈动脉、主动脉弓、胸主动脉、腹主动脉、髂动脉、肝脏，放置4%多聚甲醛中过夜，常规行HE染色，检查血管病变和相关脏器病变情况。

Conventional MR showed supraspinatus abnormalities were two cases,MR arthrography showed supraspinatus abnormalities were four cases.Conventional MR showed Labral abnormalities were three cases,MR arthrography showed Labral abnormalities were six cases.Conventional MR showed capsule abnormalities were 0 cases,MR arthrography showed capsule abnormalities were four cases.Conventional MR showed bone abnormalities were eight cases,MRarthrographyshowedboneabnormalities were eight cases.

结果：8例中，常规MRI表现冈下肌腱异常者4例，MR关节造影表现冈下肌腱异常者7例；常规MRI表现冈上肌腱异常者2例，MR关节造影表现冈上肌腱异常者4例；常规MRI表现盂唇异常者3例，MR关节造影表现盂唇异常者6例；常规MRI表现关节囊异常者0例，MR关节造影表现关节囊异常者4例；常规MRI表现骨异常者8例，MR关节造影表现骨异常者8例。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。